LOCUS       sequence-247306-        9684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses translation initiation-controlled Renilla luciferase and 
            translation elongation-controlled firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  TITLE     Elongation controlled transcripts in yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTH862-CEN-slowmaxRLuc/minCFLuc
FEATURES             Location/Qualifiers
     source          1..9684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        444..548
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(549..617,618..1409)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(767..786)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1580..2168
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2069..2088
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2322..2339
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2456..2477
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2492..2497,2498..2515,2516..2522)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2530..2546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2535..2557
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2554..2570
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2554..2570
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2591..2609
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(2657..2956)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        4836..5479
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5372..5395
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             complement(5520..5531)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     terminator      7286..7473
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7341..7365)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7538..7557)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7539..7557)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7564..7581)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7564..7580)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7573..7595)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7721..8176
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7808..7827)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8018..8039
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8307..9110)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(9111..9326)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(9350..9369)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9469..9491
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9529..9547)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(9588..9684,1..407)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 ggattttaga aagtaaataa agaaggtaga agagttacgg aatgaagaaa aaaaaataaa
       61 caaaggttta aaaaatttca acaaaaagcg tactttacat atatatttat tagacaagaa
      121 aagcagatta aatagatata cattcgatta acgataagta aaatgtaaaa tcacaggatt
      181 ttcgtgtgtg gtcttctaca cagacaagat gaaacaattc ggcattaata cctgagagca
      241 ggaagagcaa gataaaaggt agtatttgtt ggcgatcccc ctagagtctt ttacatcttc
      301 ggaaaacaaa aactattttt tctttaattt ctttttttac tttctatttt taatttatat
      361 atttatatta aaaaatttaa attataatta tttttatagc acgtgatgaa aaggacccag
      421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     1021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     1081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     1141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     1201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     1261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     1321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     1381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     1741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     2041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     2101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     2161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     2221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     2281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     2341 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     2401 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2461 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2521 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2581 ccaagctcga aattaaccct cactaaaggg aacaaaagct ggtaccgggc cccccctcga
     2641 gggcatgcga aggaaaatga gaaatatcga gggagacgat tcagaggagc aggacaaact
     2701 ataaccgact gtttgttgga ggatgccgta cataacgaac actgctgaag ctaccatgtc
     2761 tacagtttag aggaatgggt acaactcaca ggcgagggat ggtgttcact cgtgctagca
     2821 aacgcggtgg gagcaaaaag tagaatatta tcttttattc gtgaaacttc gaacactgtc
     2881 atctaaagat gctatatact aatataggca tacttgataa tgaaaactat aaatcgtaaa
     2941 gacataagag atccgcttat ttagagtgtc gaattcttat taggacattt gttcgttttt
     3001 caagactctt tcgacaaagg atttaatgta tttacccatt tcgtccggcg cgtcttcttg
     3061 ggaaaaatgc aaacctttga ctttgacaaa ttcagtgttc ggaaattttt tcgcaccttc
     3121 gacaatcgcg ttggaaaaaa aaccggggtc ggattcaata aacattttcg gcaagtcgtc
     3181 ggacgctctc aagtaggcgt tgtagtttct gacaatttgg acgacgtccg gtttaccacc
     3241 tttgaccaac ggaatttctc tcggccagga caaagtcggt cttctgactt cacctttttc
     3301 tttaaacggt tccaagtacg ccgcaaattc ttccggttcc aattttctca taattttgga
     3361 cggcaacata gtttcgacaa aaaagttgtt ttccaagacc attttttcac cttcttcgga
     3421 tttaatcaac gcaatgtctt cttcaatgtc cggccattcg tcccaggatt caatgacgtc
     3481 gacgacggat tccgcatgga caatcgcttt aattttgtct tgatgttcgt aggagtaatg
     3541 aaacgccaaa cacgcacccc agtcatgacc gacaaaaata atttttttcg gcaagttcaa
     3601 caattcaaac cacgcagtca agtatttgta atggtccaac aatctgtagg aaccgttacc
     3661 ggatttaccg gatttaccca taccaatcaa gtccggaata atacatctcg cgacgggttc
     3721 aatatgcggg acgacatgtc tccacaagta ggaggacgcc gcgttaccat gcaaaaaaat
     3781 gaccgcgttt tccgcatgtt tttcggagtc gtagtagtta ataaaggagt ccaagacgtt
     3841 catttgttta catctcgccc accattgcgg accagtaatc attctttttc tttgttccgg
     3901 gtcgtagact ttggaagtca tcccgggttt atttgtattt aatttatttt cttgagcaga
     3961 caaattggta aacaaaactt tagtaataat aatgatttaa ttaatgatag tatagggaaa
     4021 tttttattgg cgagtaaacc tggataattt gacagaaagg taaccgttac ggaaagatct
     4081 tgaataaaat tctacgggta cctgatagca attggtaaca aaacaaataa ctcttcaaaa
     4141 aactgacagt tttcaaaaaa agtaaaggac tttaattaat aagggaaaat aaattttctc
     4201 tttcaataaa tttaacacag aattctctta ataattttct aataataatc tactttaaaa
     4261 acaaaatata atcggtttag caagccattt ttcaatgatc tttaattttt taatacgata
     4321 ctgataataa cttaataaac tgaactaaaa taaaatattt tgttttgatt gcgaagtaga
     4381 tgagtgagct gtgtggctgg tgagttgtat aattcgctag tgaaaccggg gagttgattg
     4441 tagcttggta tagcttgaaa tattgtgcag aaaaagaaac aaggaagaaa gggaacgaga
     4501 acaatgacga ggaaacaaaa gattaataat tgcaggtcta tttatacttg atagcaagac
     4561 agcaaacttt tttttatttc aaattcaagt aactggaagg aaggccgtat accgttgctc
     4621 attagagagt agtgtgcgtg aatgaaggaa ggaaaaagtt tcgtgtgctt cgagataccc
     4681 ctcatcagct ctggaacaac gacatctgtt ggtgctgtct ttgtcgttaa ttttttcctt
     4741 tagtgtcttc catcattttt ttgtcattgc ggatatggtg agacaacaac gggggagaga
     4801 gaaaagaaaa aaaaagaaaa gaatccggga gtttatcatt atcaatactg ccatttcaaa
     4861 gaatacgtaa ataattaata gtagtgattt tcctaacttt atttagtcaa aaaattagcc
     4921 ttttaattct gctgtaaccc gtacatgccc aaaatagggg gcgggttaca cagaatatat
     4981 aacatcgtag gtgtctgggt gaacagttta ttcctggcat ccactaaata taatggagcc
     5041 cgctttttaa gctggcatcc agaaaaaaaa agaatcccag caccaaaata ttgttttctt
     5101 caccaaccat cagttcatag gtccattctc ttagcgcaac tacagagaac aggggcacaa
     5161 acaggcaaaa aacgggcaca acctcaatgg agtgatgcaa cctgcctgga gtaaatgatg
     5221 acacaaggca attgacccac gcatgtatct atctcatttt cttacacctt ctattacctt
     5281 ctgctctctc tgatttggaa aaagctgaaa aaaaaggttg aaaccagttc cctgaaatta
     5341 ttcccctact tgactaataa gtatataaag acggtaggta ttgattgtaa ttctgtaaat
     5401 ctatttctta aacttcttaa attctacttt tatagttagt ctttttttta gttttaaaac
     5461 accaagaact tagtttcgac ggatccatgg aggacgcaaa aaacataaaa aaagggcctg
     5521 cgcccttcta tcctctcgag gatgggacgg cgggggagca gctccataaa gcgatgaaaa
     5581 ggtacgcact cgtgcctggg acgatagcgt ttacggatgc acatatagag gtgaacataa
     5641 cgtacgcgga gtacttcgag atgtcggtgc ggctcgcaga ggcgatgaaa cggtatgggc
     5701 tcaatacgaa tcacaggata gtagtatgct cggagaactc gctccagttc tttatgcctg
     5761 tgctcggggc gctctttata ggggtggcag tggcgcccgc gaacgacata tataatgagc
     5821 gggagctcct caactcgatg aacatatcgc agcctacggt agtgtttgtg tcgaaaaaag
     5881 ggctccagaa aatactcaac gtgcagaaaa aactccccat aatacagaaa ataataataa
     5941 tggattcgaa aacggattac caggggtttc agtcgatgta cacgttcgta acgtcgcatc
     6001 tccctcccgg gtttaatgag tacgattttg tacccgagtc gtttgatcgg gacaaaacga
     6061 tagcactcat aatgaattcg tcgggatcga cggggctccc taaaggggtg gcactccctc
     6121 ataggacggc atgcgtaagg ttctcgcatg caagggaccc tatatttggg aatcagataa
     6181 tacctgatac ggcgatactc tcggtggtgc ccttccatca cgggtttggg atgtttacga
     6241 cgctcgggta tctcatatgt gggtttcggg tagtactcat gtataggttt gaggaggagc
     6301 tctttctccg gtcgctccag gattacaaaa tacagtcggc gctcctcgta cccacgctct
     6361 tttcgttctt cgcaaaatcg acgctcatag acaaatacga tctctcgaat ctccacgaga
     6421 tagcgtcggg gggggcacct ctctcgaaag aggtagggga ggcggtggca aaacggttcc
     6481 atctccccgg gatacggcag gggtatgggc tcacggagac gacgtcggcg atactcataa
     6541 cgcccgaggg ggatgataaa cctggggcgg tagggaaagt ggtgcccttt tttgaggcga
     6601 aagtggtgga tctcgatacg gggaaaacgc tcggggtgaa tcagaggggg gagctctgtg
     6661 taagggggcc tatgataatg tcggggtatg taaacaatcc tgaggcgacg aacgcactca
     6721 tagacaaaga tgggtggctc cattcggggg acatagcgta ctgggacgag gacgagcact
     6781 tcttcatagt ggaccggctc aaatcgctca taaaatacaa agggtatcag gtggcacccg
     6841 cggagctcga gtcgatactc ctccagcacc ccaacatatt cgacgcgggg gtggcagggc
     6901 tccccgacga tgacgcaggg gagctccccg cagcagtggt ggtgctcgag cacgggaaaa
     6961 cgatgacgga gaaagagata gtggattacg tagcatcgca ggtaacgacg gcgaaaaaac
     7021 tccggggggg ggtggtgttt gtggacgagg tacctaaagg gctcacgggg aaactcgacg
     7081 caaggaaaat aagggagata ctcataaaag caaaaaaagg ggggaaataa gtcgacctgc
     7141 aggcatgcaa gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt
     7201 cggcttgtct accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag
     7261 atacgttgtt gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
     7321 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
     7381 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca
     7441 tgaggtcgct cttattgacc acacctctac cggcatgccg gtcgaaattc ccctacccta
     7501 tgaaaagcgg ccgccaccgc ggtggagctc caattcgccc tatagtgagt cgtattacaa
     7561 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7621 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7681 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg acgcgccctg tagcggcgca
     7741 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     7801 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     7861 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     7921 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     7981 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8041 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8101 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8161 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     8221 accgcatagg gtaataactg atataattaa attgaagctc taatttgtga gtttagtata
     8281 catgcattta cttataatac agttttttag ttttgctggc cgcatcttct caaatatgct
     8341 tcccagcctg cttttctgta acgttcaccc tctaccttag catcccttcc ctttgcaaat
     8401 agtcctcttc caacaataat aatgtcagat cctgtagaga ccacatcatc cacggttcta
     8461 tactgttgac ccaatgcgtc tcccttgtca tctaaaccca caccgggtgt cataatcaac
     8521 caatcgtaac cttcatctct tccacccatg tctctttgag caataaagcc gataacaaaa
     8581 tctttgtcgc tcttcgcaat gtcaacagta cccttagtat attctccagt agatagggag
     8641 cccttgcatg acaattctgc taacatcaaa aggcctctag gttcctttgt tacttcttct
     8701 gccgcctgct tcaaaccgct aacaatacct gggcccacca caccgtgtgc attcgtaatg
     8761 tctgcccatt ctgctattct gtatacaccc gcagagtact gcaatttgac tgtattacca
     8821 atgtcagcaa attttctgtc ttcgaagagt aaaaaattgt acttggcgga taatgccttt
     8881 agcggcttaa ctgtgccctc catggaaaaa tcagtcaaga tatccacatg tgtttttagt
     8941 aaacaaattt tgggacctaa tgcttcaact aactccagta attccttggt ggtacgaaca
     9001 tccaatgaag cacacaagtt tgtttgcttt tcgtgcatga tattaaatag cttggcagca
     9061 acaggactag gatgagtagc agcacgttcc ttatatgtag ctttcgacat gatttatctt
     9121 cgtttcctgc aggtttttgt tctgtgcagt tgggttaaga atactgggca atttcatgtt
     9181 tcttcaacac tacatatgcg tatatatacc aatctaagtc tgtgctcctt ccttcgttct
     9241 tccttctgtt cggagattac cgaatcaaaa aaatttcaag gaaaccgaaa tcaaaaaaaa
     9301 gaataaaaaa aaaatgatga attgaaaagg tggtatggtg cactctcagt acaatctgct
     9361 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     9421 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     9481 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     9541 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacggat cgcttgcctg
     9601 taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt
     9661 tatttatttt tatgttttgt attt
//
