LOCUS       sequence-247328-        3221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for loxP-KIURA3-loxP, Type 6 (yeast marker) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0519
FEATURES             Location/Qualifiers
     source          1..3221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    30..63
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1516..1549
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(1586..1620)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1691..2350)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2265..2284
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2532..3120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3021..3040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctcatacag caggtcgaca acccttaata taacttcgta taatgtatgc tatacgaagt
       61 tattaggtct agagatccca atacaacaga tcacgtgatc ttttgtaaga tgaagttgaa
      121 gtgagtgttg caccgtgcca atgcaggtgg ctattagatt aaatatgtga tttgttctat
      181 taagtttcct gtataattaa tggggagcgc tgattctctt ttggtacgct tcccatccag
      241 catttctgta tctttcacct tcaaccttag gatctctacc cttggcgaaa agtcctctgc
      301 caacaatgat gatatctgat ccaccactta caacttcgtc gacggttctg tactgctgac
      361 ccaatgcatc gcctttgtcg tctaaaccta cacctggggt catgattagc caatcaaacc
      421 cttcttctct tcctcccata tcgttctgag caatgaaccc aataacgaaa tctttatcac
      481 tctttgcaat atcaacggta cccttagtat attcaccgtg tgctagagaa cccttggaag
      541 acaattcagc aagcatcaat aatccccttg gttctttggt gacctcttgc gcaccttgtt
      601 tcaagccagc aacaatacca gcaccagtaa ccccgtgggc gttggtgata tcagaccatt
      661 ctgcgatacg gtaaacgccc gatgtatatt gtaatttgac tgtgttaccg atatcggcga
      721 attttctgtc ctcaaatatc aagaacttgt atttctctgc caatgctttc aatggaacga
      781 cagtaccctc ataactgaaa tcatccaaga tatcaacgtg tgttttcaaa aggcaaatgt
      841 atggacccaa cgtttcaaca agtttcaata gctcatcagt cgaacgaacg tcaagagaag
      901 cacacaaatt ggtcttcttt tcatccatta aacgtaaaag tttcgatgca accggacttg
      961 catgagtctc agctctactg gtatatgatt ttgtggacat ggtgcaacta attgacggga
     1021 gtgtattgac gctggcgtac tggctttcac aaaatggccc aatcacaacc acatcttaga
     1081 tagttgaaat gactttagat aacatcaatt gagatgagct taatcatgtc aaagctaaaa
     1141 gtgtcaccat gaacgacaat tcttaagcaa atcacgtgat atagatccac gaataaccac
     1201 catttgatgc tcgaggcaag taatgtgtgt aaaaaaatgc gttaccacca tccaatgcag
     1261 accgatcttc tacccagaat cacatatatt tatgtaccga gtaccttttt tctatcttcc
     1321 aattgcttct cccatatgat tgtctccgta agctcgaaat ttctaagttg gattttaatc
     1381 ttcacgcagg atgacagttc gatgagcttc tgaggagtgt ttagaacata atcagtttat
     1441 ccatggtcta tctcttcttg tcgctttttc tcctcgatag aacctaaata aaacgagctc
     1501 tcgagaaccc ttaatataac ttcgtataat gtatgctata cgaagttatt aggtgatatc
     1561 agatccactg agttgagacc gaccaataaa aaacgcccgg cggcaaccga gcgttctgaa
     1621 caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct
     1681 cgatatcaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     1741 ttctgccgac atggaagcca tcacaaacgg catgatgaac ctgaatcgcc agcggcatca
     1801 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     1861 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgaaacga
     1921 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     1981 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     2041 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     2101 tcaccagctc accgtctttc attgccatac gaaattccgg atgagcattc atcaggcggg
     2161 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     2221 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     2281 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     2341 ttttctccat tttagcttcc ttagctcctg aaaatctcga taactcaaaa aatacgcccg
     2401 gtagtgatct tatttcatta tggtgaaagt tggaacctct tacgtgcccg atcaatcatg
     2461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3181 ttctttcctg cgttatcccc tgattctgtg gataaccgta g
//
