LOCUS       sequence-247331-        2363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for pZF(3BS), Type 2 (promoter) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0528
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     665..688
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     terminator      complement(728..762)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(833..1492)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1407..1426
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1674..2262
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2163..2182
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcaa acgcgaggca agctaaacag atctttatat tgaattttca aaaattctta
       61 cttttttttt ggatggacgc aaagaagttt aataatcata ttacatggca ttaccaccat
      121 atacatatcc atatacatat ccatatctaa tcttacttat atgttgtgga aatgtaaaga
      181 gccccattat cttagcctaa aaaaaccttc tctttggaac tttcagtaat acgcttaact
      241 gctcattgct atattgaagt gcgcgcgcgc gtgggcgagc gtgggcgagc gtgggcgtct
      301 agaccgtgcg tcctcgtctt caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg
      361 cactgctccg aacaataaag attctacaat actagctttt atggttatga agaggaaaaa
      421 ttggcagtaa cctggcccca caaaccttca aattaacgaa tcaaattaac aaccatagga
      481 tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg aagcgatgat
      541 ttttgatcta ttaacagata tataaatgga aaagctgcat aaccacttta actaatactt
      601 tcaacatttt cagtttgtat tacttcttat tcaaatgtca taaaagtatc aacaaaaaat
      661 tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ctataggtac tatgtgagac
      721 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
      781 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
      841 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
      901 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      961 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1021 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     1081 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1141 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1201 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1261 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1321 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1381 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1441 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1501 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1561 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     1621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1801 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     1861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     2101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     2281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     2341 cctgattctg tggataaccg tag
//
