LOCUS       sequence-247332-        3810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Part plasmid for Zif268DBD-CRY2, Type 3 (coding sequence) part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0529
FEATURES             Location/Qualifiers
     source          1..3810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             320..2155
                     /codon_start=1
                     /product="Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKE
                     PGLCPSVSSNDQQVPSAVRYNGSKRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSV
                     FFVSQSCSLASEGKNLEGIQDSSDQITTSLGKNGCK"
     terminator      complement(2175..2209)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2280..2939)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2854..2873
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3121..3709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3610..3629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggccccca agaagaagag gaaggttggt attcacgggg gtacccgccc
       61 atatgcttgc cctgtcgagt cctgcgatcg ccgcttttct cgctcggatg agcttacccg
      121 ccatatccgc atccataccg gtcagaagcc cttccagtgt cgaatctgca tgcgtaactt
      181 cagtcgtagt gaccacctta ccacccacat ccgcacccac acaggcgaga agccttttgc
      241 ctgtgacatt tgtgggagga agtttgccag gagtgatgaa cgcaagaggc ataccaaaat
      301 ccatacaggt gctagcttca tgaagatgga caaaaagact atagtttggt ttagaagaga
      361 tctaaggatt gaggataatc ctgcattagc agcagctgct cacgaaggat ctgtttttcc
      421 tgtcttcatt tggtgtcctg aagaagaagg acagttttat cctggaagag cttcaagatg
      481 gtggatgaaa caatcacttg ctcacttatc tcaatccttg aaggctcttg gatctgacct
      541 cactttaatc aaaacccaca acacgatttc agcgatcttg gattgtatcc gcgttaccgg
      601 tgctacaaaa gtcgtcttta accacctcta tgatcctgtt tcgttagttc gggaccatac
      661 cgtaaaggag aagctggtgg aacgtgggat ctctgtgcaa agctacaatg gagatctatt
      721 gtatgaaccg tgggagatat actgcgaaaa gggcaaacct tttacgagtt tcaattctta
      781 ctggaagaaa tgcttagata tgtcgattga atccgttatg cttcctcctc cttggcggtt
      841 gatgccaata actgcagcgg ctgaagcgat ttgggcgtgt tcgattgaag aactagggct
      901 ggagaatgag gccgagaaac cgagcaatgc gttgttaact agagcttgga gtccaggatg
      961 gagcaatgct gataagttac taaatgagtt catcgagaag cagttgatag attatgcaaa
     1021 gaacagcaag aaagttgttg ggaattctac ttcactactt tctccgtatc tccatttcgg
     1081 ggaaataagc gtcagacacg ttttccagtg tgcccggatg aaacaaatta tatgggcaag
     1141 agataagaac agtgaaggag aagaaagtgc agatcttttt cttaggggaa tcggtttaag
     1201 agagtattct cggtatatat gtttcaactt cccgtttact cacgagcaat cgttgttgag
     1261 tcatcttcgg tttttccctt gggatgctga tgttgataag ttcaaggcct ggagacaagg
     1321 caggaccggt tatccgttgg tggatgccgg aatgagagag ctttgggcta ccggatggat
     1381 gcataacaga ataagagtga ttgtttcaag ctttgctgtg aagtttcttc tccttccatg
     1441 gaaatgggga atgaagtatt tctgggatac acttttggat gctgatttgg aatgtgacat
     1501 ccttggctgg cagtatatct ctgggagtat ccccgatggc cacgagcttg atcgcttgga
     1561 caatcccgcg ttacaaggcg ccaaatatga cccagaaggt gagtacataa ggcaatggct
     1621 tcccgagctt gcgagattgc caactgaatg gatccatcat ccatgggacg ctcctttaac
     1681 cgtactcaaa gcttctggtg tggaactcgg aacaaactat gcgaaaccca ttgtagacat
     1741 cgacacagct cgtgagctac tagctaaagc tatttcaaga acccgtgaag cacagatcat
     1801 gatcggagca gcacctgatg agattgtagc agatagcttc gaggccttag gggctaatac
     1861 cattaaagaa cctggtcttt gcccatctgt gtcttctaat gaccaacaag taccttcggc
     1921 tgttcgttac aacgggtcaa agagagtgaa acctgaggaa gaagaagaga gagacatgaa
     1981 gaaatctagg ggattcgatg aaagggagtt gttttcgact gctgaatctt cttcttcttc
     2041 gagtgtgttt ttcgtttcgc agtcttgctc gttggcatca gaagggaaga atctggaagg
     2101 tattcaagat tcatctgatc agattactac aagtttggga aaaaatggtt gcaaaggatc
     2161 ctgagaccag accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat
     2221 ggagttctga ggtcattact ggatctatca acaggagtcc aagcgagctc gatatcaaat
     2281 tacgccccgc cctgccactc atcgcagtac tgttgtaatt cattaagcat tctgccgaca
     2341 tggaagccat cacaaacggc atgatgaacc tgaatcgcca gcggcatcag caccttgtcg
     2401 ccttgcgtat aatatttgcc catggtgaaa acgggggcga agaagttgtc catattggcc
     2461 acgtttaaat caaaactggt gaaactcacc cagggattgg ctgaaacgaa aaacatattc
     2521 tcaataaacc ctttagggaa ataggccagg ttttcaccgt aacacgccac atcttgcgaa
     2581 tatatgtgta gaaactgccg gaaatcgtcg tggtattcac tccagagcga tgaaaacgtt
     2641 tcagtttgct catggaaaac ggtgtaacaa gggtgaacac tatcccatat caccagctca
     2701 ccgtctttca ttgccatacg aaattccgga tgagcattca tcaggcgggc aagaatgtga
     2761 ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata
     2821 tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt
     2881 tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt
     2941 ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt
     3001 atttcattat ggtgaaagtt ggaacctctt acgtgcccga tcaatcatga ccaaaatccc
     3061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     3421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     3481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     3541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     3601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     3661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     3721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3781 gttatcccct gattctgtgg ataaccgtag
//
