LOCUS       sequence-247340-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Spacer cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0619
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(321..1112,1113..1181))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     944..963
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1182..1286)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1389..1977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1878..1897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgactaga gatctatgtg aggatcctaa ctcgagatcg agctgagcat gagacggaaa
       61 tctgctcgtc agtggtgctc acactgacga atcatgtaca gatcataccg atgactgcct
      121 ggcgactcac aactaagcaa gacagccgga accagcgccg gcgaacacca ctgcatatat
      181 ggcatatcac aacagtccaa ctagtgcact gcagtacagc ggccgcgatt atcaaaaagg
      241 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
      301 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
      361 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
      421 gagggcttac catctggccc cagtgctgca atgataccgc gggacccacg ctcaccggct
      481 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
      541 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
      601 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
      661 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
      721 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
      781 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
      841 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
      901 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
      961 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     1021 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     1081 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     1141 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     1201 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1261 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc
     1321 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1381 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1441 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1501 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1561 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1621 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1681 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1741 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1801 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1861 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1921 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1981 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2041 tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg ccgcccctga attcgcatct
     2101 agaataaatc gcagccaagt gagtgaatag atgacgcacc acggtcagac acggccacat
     2161 cgtatctcac aggagcaagc gcgataggag cactcacaca tagtacggtg atccgctgac
     2221 tcctttgccc aaataagacg tgagcccgtc tcagatgcac acactggctt aagatgac
//
