LOCUS       sequence-247344-        4996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRPL18B-Zif268DBD-CRY2PHR cassette plasmid flanked by ConL1 and 
            ConR2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 4996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0623
FEATURES             Location/Qualifiers
     source          1..4996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             717..737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1020..2513
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             complement(join(3039..3830,3831..3899))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3662..3681
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3900..4004)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4107..4695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4596..4615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgaagagg atgtccaata ttttttttaa ggaataagga tacttcaaga ctagattccc
       61 ccctgcattc ccatcagaac cgtaaacctt ggcgctttcc ttgggaagta ttcaagaagt
      121 gccttgtccg gtttctgtgg ctcacaaacc agcgcgcccg atatggcttt cttttcactt
      181 atgaatgtac cagtacggga caattagaac gctcctgtaa caatctcttt gcaaatgtgg
      241 ggttacattc taaccatgtc acactgctga cgaaattcaa agtaaaaaaa aatgggacca
      301 cgtcttgaga acgatagatt ttctttattt tacattgaac agtcgttgtc tcagcgcgct
      361 ttatgttttc attcatactt catattataa aataacaaaa gaagaatttc atattcacgc
      421 ccaagaaatc aggctgcttt ccaaatgcaa ttgacacttc attagccatc acacaaaact
      481 ctttcttgct ggagcttctt ttaaaaaaga cctcagtaca ccaaacacgt tacccgacct
      541 cgttatttta cgacaactat gataaaattc tgaagaaaaa ataaaaaaat tttcatactt
      601 cttgctttta tttaaaccat tgaatgattt cttttgaaca aaactacctg tttcaccaaa
      661 ggaaatagaa agaaaaaatc aattagaaga aaacaaaaaa caaaagatct atggccccca
      721 agaagaagag gaaggttggt attcacgggg gtacccgccc atatgcttgc cctgtcgagt
      781 cctgcgatcg ccgcttttct cgctcggatg agcttacccg ccatatccgc atccataccg
      841 gtcagaagcc cttccagtgt cgaatctgca tgcgtaactt cagtcgtagt gaccacctta
      901 ccacccacat ccgcacccac acaggcgaga agccttttgc ctgtgacatt tgtgggagga
      961 agtttgccag gagtgatgaa cgcaagaggc ataccaaaat ccatacaggt gctagcttca
     1021 tgaagatgga caaaaagact atagtttggt ttagaagaga tctaaggatt gaggataatc
     1081 ctgcattagc agcagctgct cacgaaggat ctgtttttcc tgtcttcatt tggtgtcctg
     1141 aagaagaagg acagttttat cctggaagag cttcaagatg gtggatgaaa caatcacttg
     1201 ctcacttatc tcaatccttg aaggctcttg gatctgacct cactttaatc aaaacccaca
     1261 acacgatttc agcgatcttg gattgtatcc gcgttaccgg tgctacaaaa gtcgtcttta
     1321 accacctcta tgatcctgtt tcgttagttc gggaccatac cgtaaaggag aagctggtgg
     1381 aacgtgggat ctctgtgcaa agctacaatg gagatctatt gtatgaaccg tgggagatat
     1441 actgcgaaaa gggcaaacct tttacgagtt tcaattctta ctggaagaaa tgcttagata
     1501 tgtcgattga atccgttatg cttcctcctc cttggcggtt gatgccaata actgcagcgg
     1561 ctgaagcgat ttgggcgtgt tcgattgaag aactagggct ggagaatgag gccgagaaac
     1621 cgagcaatgc gttgttaact agagcttgga gtccaggatg gagcaatgct gataagttac
     1681 taaatgagtt catcgagaag cagttgatag attatgcaaa gaacagcaag aaagttgttg
     1741 ggaattctac ttcactactt tctccgtatc tccatttcgg ggaaataagc gtcagacacg
     1801 ttttccagtg tgcccggatg aaacaaatta tatgggcaag agataagaac agtgaaggag
     1861 aagaaagtgc agatcttttt cttaggggaa tcggtttaag agagtattct cggtatatat
     1921 gtttcaactt cccgtttact cacgagcaat cgttgttgag tcatcttcgg tttttccctt
     1981 gggatgctga tgttgataag ttcaaggcct ggagacaagg caggaccggt tatccgttgg
     2041 tggatgccgg aatgagagag ctttgggcta ccggatggat gcataacaga ataagagtga
     2101 ttgtttcaag ctttgctgtg aagtttcttc tccttccatg gaaatgggga atgaagtatt
     2161 tctgggatac acttttggat gctgatttgg aatgtgacat ccttggctgg cagtatatct
     2221 ctgggagtat ccccgatggc cacgagcttg atcgcttgga caatcccgcg ttacaaggcg
     2281 ccaaatatga cccagaaggt gagtacataa ggcaatggct tcccgagctt gcgagattgc
     2341 caactgaatg gatccatcat ccatgggacg ctcctttaac cgtactcaaa gcttctggtg
     2401 tggaactcgg aacaaactat gcgaaaccca ttgtagacat cgacacagct cgtgagctac
     2461 tagctaaagc tatttcaaga acccgtgaag cacagatcat gatcggagca gcagcccggg
     2521 gatcctaact cgaggccaat tggtgcggca attgataata acgaaaatgt cttttaatga
     2581 tctgggtata atgaggaatt ttccgaacgt ttttacttta tatatatata tacatgtaac
     2641 atatattcta tacgctatag agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga
     2701 gtttcacttc ttgattatcg ctaacactaa tggttgaagt actgctactt taattttatg
     2761 ctggatgtga gacgataaat cgcagccaag tgagtgaata gatgacgcac cacggtcaga
     2821 cacggccaca tcgtatctca caggagcaag cgcgatagga gcactcacac atagtacggt
     2881 gatccgctga ctcctttgcc caaataagac gtgagccact agtgcactgc agtacagcgg
     2941 ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3001 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3061 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3121 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcgg
     3181 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     3241 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     3301 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     3361 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3421 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3481 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3541 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3601 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3661 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3721 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3781 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3841 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3901 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3961 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4021 gtgccacctg tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     4081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     4141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     4201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     4261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     4321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     4381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     4441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     4501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     4561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc
     4801 gcccctgaat tcgcatctag aacggggtca tcacggctca tcatgcgcca aacaaatgtg
     4861 tgcaatacac gctcggatga ctgcatgatg accgcactga ctggggacag cagatccacc
     4921 taagcctgtg agagaagcag acacccgaca gatcaaggca gttacgtctc accaaaccag
     4981 atgtcaacac agctac
//
