LOCUS       sequence-247345-        3890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0624
FEATURES             Location/Qualifiers
     source          1..3890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(25..129)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      232..820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     721..740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1777..1800
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             1824..2534
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      2550..2737
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2605..2629)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3054..3845,3846..3890,1..24))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3677..3696
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
       61 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      121 ggttccgcgc acatttcccc gaaaagtgcc acctgtcatg accaaaatcc cttaacgtga
      181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
      361 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
      421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
      481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      601 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      841 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      901 tgattctgtg gataaccgtg cggccgcccc tgaattcgca tctagaataa atcgcagcca
      961 agtgagtgaa tagatgacgc accacggtca gacacggcca catcgtatct cacaggagca
     1021 agcgcgatag gagcactcac acatagtacg gtgatccgct gactcctttg cccaaataag
     1081 acgtgagccc gtctcagatg cacacactgg cttaagatga caacgcgagg caagctaaac
     1141 agatctttat attgaatttt caaaaattct tacttttttt ttggatggac gcaaagaagt
     1201 ttaataatca tattacatgg cattaccacc atatacatat ccatatacat atccatatct
     1261 aatcttactt atatgttgtg gaaatgtaaa gagccccatt atcttagcct aaaaaaacct
     1321 tctctttgga actttcagta atacgcttaa ctgctcattg ctatattgaa gtgcgcgcgc
     1381 gcgtgggcga gcgtgggcga gcgtgggcgt ctagaccgtg cgtcctcgtc ttcaccggtc
     1441 gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa agattctaca
     1501 atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc cacaaacctt
     1561 caaattaacg aatcaaatta acaaccatag gatgataatg cgattagttt tttagcctta
     1621 tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga tatataaatg
     1681 gaaaagctgc ataaccactt taactaatac tttcaacatt ttcagtttgt attacttctt
     1741 attcaaatgt cataaaagta tcaacaaaaa attgttaata tacctctata ctttaacgtc
     1801 aaggagaaaa aactataggt actatggtgt ccaaaggaga ggagttaatc aaggaaaaca
     1861 tgagaatgaa agttgtcatg gagggctccg ttaatggtca ccaattcaag tgtacagggg
     1921 aaggtgaagg taatccttac atgggtacac aaactatgag aattaaagta attgaaggcg
     1981 gaccactacc atttgcattt gacattctgg caacgtcatt catgtacgga tcacgaactt
     2041 tcatcaagta ccctaaaggt ataccagact ttttcaagca atcttttcca gagggtttta
     2101 catgggaaag ggttacaaga tacgaagatg ggggtgtcgt cacagttatg caagatactt
     2161 cattagaaga tggctgcctt gtctatcatg tgcaagtaag aggggtgaat tttccttcta
     2221 acggacctgt gatgcagaaa aagaccaaag gttgggaacc aaatactgaa atgatgtacc
     2281 cagctgatgg aggtttgaga ggctacacac acatggcgct taaagttgat ggtggaggtc
     2341 atttgtcttg tagttttgtt accacttatc gttctaaaaa gactgttggc aatatcaaaa
     2401 tgccaggaat acatgctgta gaccacagac tagaaagact cgaagagagc gataacgaaa
     2461 tgttcgttgt acagagagag catgccgtag ccaaatttgc tggcttaggc ggtggtatgg
     2521 atgaattgta taagggatcc taactcgagg cgaatttctt atgatttatg atttttatta
     2581 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
     2641 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
     2701 agcatgaggt cgctcttatt gaccacacct ctaccggcat gccgagcaaa tgcctgcaaa
     2761 tcgctcccca tttcgctgag catgagacgg aaatctgctc gtcagtggtg ctcacactga
     2821 cgaatcatgt acagatcata ccgatgactg cctggcgact cacaactaag caagacagcc
     2881 ggaaccagcg ccggcgaaca ccactgcata tatggcatat cacaacagtc caactagtgc
     2941 actgcagtac agcggccgcg attatcaaaa aggatcttca cctagatcct tttaaattaa
     3001 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3061 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3121 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3181 gcaatgatac cgcgggaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3241 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3301 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3361 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3421 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3481 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3541 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3601 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3661 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3721 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     3781 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     3841 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
//
