LOCUS       sequence-247346-        3898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pREV1-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0625
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(14..118)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      221..809
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     710..729
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             1821..2531
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      2547..2734
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2602..2626)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(3051..3842,3843..3898,1..13))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3674..3693
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
       61 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
      121 catttccccg aaaagtgcca cctgtcatga ccaaaatccc ttaacgtgag ttttcgttcc
      181 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
      241 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
      301 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
      361 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
      421 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
      481 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
      541 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
      601 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
      661 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
      721 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
      781 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
      841 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
      901 ataaccgtgc ggccgcccct gaattcgcat ctagaataaa tcgcagccaa gtgagtgaat
      961 agatgacgca ccacggtcag acacggccac atcgtatctc acaggagcaa gcgcgatagg
     1021 agcactcaca catagtacgg tgatccgctg actcctttgc ccaaataaga cgtgagcccg
     1081 tctcagatgc acacactggc ttaagatgac aacggtgttg ttatccgata caaccggata
     1141 tttttctttt aatgagtcta aaccgtgata gcttcaggtt aatacaatca aaaaaagctc
     1201 aaatattctt ttaatgccgc gttcacagat tccaattgaa tacaactagg tagttcatta
     1261 tatgaagcct ttgctactat ttttcactat agtctgcctt caccttaatg cagacatcca
     1321 catattttaa tcactttaaa ataaaaagga agatatatta gaagctatga tccaatctgt
     1381 aagccagatt aaaattcacg aactcttctt tcatttgaat tgaatgcttt gagttggggt
     1441 agattatcgc aaattactca tcacatttat tgactacgaa cttgctgatg tccttttttt
     1501 atttatattt ttcttcagtg aagcgatttt ttttttacac agaccaagac ggaaaaaagt
     1561 agctaaggaa gaaaacaaaa tcatgaaaaa aatgtgaagt gatcatgcac atcgcatcaa
     1621 cttaaacatt ggcttagaga tatatagagt tagagtttac ggcaaccttt aagcaccaat
     1681 accttttggc atagtctaaa gacctggttc ttaattttaa acaaatttaa ctaaagattt
     1741 ccctatcaaa gaagtaacga gttgacagat tttctcaaaa taaatcgata ctgcatttct
     1801 aggcatatcc agcgagatct atggtgtcca aaggagagga gttaatcaag gaaaacatga
     1861 gaatgaaagt tgtcatggag ggctccgtta atggtcacca attcaagtgt acaggggaag
     1921 gtgaaggtaa tccttacatg ggtacacaaa ctatgagaat taaagtaatt gaaggcggac
     1981 cactaccatt tgcatttgac attctggcaa cgtcattcat gtacggatca cgaactttca
     2041 tcaagtaccc taaaggtata ccagactttt tcaagcaatc ttttccagag ggttttacat
     2101 gggaaagggt tacaagatac gaagatgggg gtgtcgtcac agttatgcaa gatacttcat
     2161 tagaagatgg ctgccttgtc tatcatgtgc aagtaagagg ggtgaatttt ccttctaacg
     2221 gacctgtgat gcagaaaaag accaaaggtt gggaaccaaa tactgaaatg atgtacccag
     2281 ctgatggagg tttgagaggc tacacacaca tggcgcttaa agttgatggt ggaggtcatt
     2341 tgtcttgtag ttttgttacc acttatcgtt ctaaaaagac tgttggcaat atcaaaatgc
     2401 caggaataca tgctgtagac cacagactag aaagactcga agagagcgat aacgaaatgt
     2461 tcgttgtaca gagagagcat gccgtagcca aatttgctgg cttaggcggt ggtatggatg
     2521 aattgtataa gggatcctaa ctcgaggcga atttcttatg atttatgatt tttattatta
     2581 aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag gttttaaaac
     2641 gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct caggtatagc
     2701 atgaggtcgc tcttattgac cacacctcta ccggcatgcc gagcaaatgc ctgcaaatcg
     2761 ctccccattt cgctgagcat gagacggaaa tctgctcgtc agtggtgctc acactgacga
     2821 atcatgtaca gatcataccg atgactgcct ggcgactcac aactaagcaa gacagccgga
     2881 accagcgccg gcgaacacca ctgcatatat ggcatatcac aacagtccaa ctagtgcact
     2941 gcagtacagc ggccgcgatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3001 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3061 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3121 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3181 atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3241 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3301 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3361 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3421 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3481 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3541 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3601 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3661 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3721 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3781 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3841 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaat
//
