LOCUS       sequence-247347-        3898 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pRPL18B-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0626
FEATURES             Location/Qualifiers
     source          1..3898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      61..248
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(116..140)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(join(565..1356,1357..1425))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1188..1207
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1426..1530)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1633..2221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2122..2141
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(3233..3898,1..45)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
ORIGIN
        1 gccaaatttg ctggcttagg cggtggtatg gatgaattgt ataagggatc ctaactcgag
       61 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
      121 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
      181 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
      241 tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctga gcatgagacg
      301 gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat accgatgact
      361 gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac accactgcat
      421 atatggcata tcacaacagt ccaactagtg cactgcagta cagcggccgc gattatcaaa
      481 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
      541 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
      601 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
      661 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc cacgctcacc
      721 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
      781 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      841 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      901 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      961 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     1021 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     1081 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     1141 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     1201 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     1261 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     1321 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     1381 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     1441 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     1501 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcat
     1561 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1681 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1741 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1801 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1861 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1921 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1981 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2041 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     2101 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     2161 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     2221 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     2281 gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgccc ctgaattcgc
     2341 atctagaata aatcgcagcc aagtgagtga atagatgacg caccacggtc agacacggcc
     2401 acatcgtatc tcacaggagc aagcgcgata ggagcactca cacatagtac ggtgatccgc
     2461 tgactccttt gcccaaataa gacgtgagcc cgtctcagat gcacacactg gcttaagatg
     2521 acaacgaaga ggatgtccaa tatttttttt aaggaataag gatacttcaa gactagattc
     2581 ccccctgcat tcccatcaga accgtaaacc ttggcgcttt ccttgggaag tattcaagaa
     2641 gtgccttgtc cggtttctgt ggctcacaaa ccagcgcgcc cgatatggct ttcttttcac
     2701 ttatgaatgt accagtacgg gacaattaga acgctcctgt aacaatctct ttgcaaatgt
     2761 ggggttacat tctaaccatg tcacactgct gacgaaattc aaagtaaaaa aaaatgggac
     2821 cacgtcttga gaacgataga ttttctttat tttacattga acagtcgttg tctcagcgcg
     2881 ctttatgttt tcattcatac ttcatattat aaaataacaa aagaagaatt tcatattcac
     2941 gcccaagaaa tcaggctgct ttccaaatgc aattgacact tcattagcca tcacacaaaa
     3001 ctctttcttg ctggagcttc ttttaaaaaa gacctcagta caccaaacac gttacccgac
     3061 ctcgttattt tacgacaact atgataaaat tctgaagaaa aaataaaaaa attttcatac
     3121 ttcttgcttt tatttaaacc attgaatgat ttcttttgaa caaaactacc tgtttcacca
     3181 aaggaaatag aaagaaaaaa tcaattagaa gaaaacaaaa aacaaaagat ctatggtgtc
     3241 caaaggagag gagttaatca aggaaaacat gagaatgaaa gttgtcatgg agggctccgt
     3301 taatggtcac caattcaagt gtacagggga aggtgaaggt aatccttaca tgggtacaca
     3361 aactatgaga attaaagtaa ttgaaggcgg accactacca tttgcatttg acattctggc
     3421 aacgtcattc atgtacggat cacgaacttt catcaagtac cctaaaggta taccagactt
     3481 tttcaagcaa tcttttccag agggttttac atgggaaagg gttacaagat acgaagatgg
     3541 gggtgtcgtc acagttatgc aagatacttc attagaagat ggctgccttg tctatcatgt
     3601 gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg atgcagaaaa agaccaaagg
     3661 ttgggaacca aatactgaaa tgatgtaccc agctgatgga ggtttgagag gctacacaca
     3721 catggcgctt aaagttgatg gtggaggtca tttgtcttgt agttttgtta ccacttatcg
     3781 ttctaaaaag actgttggca atatcaaaat gccaggaata catgctgtag accacagact
     3841 agaaagactc gaagagagcg ataacgaaat gttcgttgta cagagagagc atgccgta
//
