LOCUS       sequence-247348-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pTDH3-mRUBY2 cassette plasmid flanked by ConL2 and ConRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0627
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(110..901,902..970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     733..752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(971..1075)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1178..1766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1667..1686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2085..2751
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2621..2644
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             2758..3468
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      3484..3671
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(3539..3563)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
ORIGIN
        1 cagtacagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
       61 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      121 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
      181 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
      241 tgataccgcg ggacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
      301 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
      361 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
      421 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
      481 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
      541 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
      601 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
      661 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
      721 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
      781 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
      841 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
      901 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
      961 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1021 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1081 ttccccgaaa agtgccacct gtcatgacca aaatccctta acgtgagttt tcgttccact
     1141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1861 accgtgcggc cgcccctgaa ttcgcatcta gaataaatcg cagccaagtg agtgaataga
     1921 tgacgcacca cggtcagaca cggccacatc gtatctcaca ggagcaagcg cgataggagc
     1981 actcacacat agtacggtga tccgctgact cctttgccca aataagacgt gagcccgtct
     2041 cagatgcaca cactggctta agatgacaac gcagttcgag tttatcatta tcaatactgc
     2101 catttcaaag aatacgtaaa taattaatag tagtgatttt cctaacttta tttagtcaaa
     2161 aaattagcct tttaattctg ctgtaacccg tacatgccca aaataggggg cgggttacac
     2221 agaatatata acatcgtagg tgtctgggtg aacagtttat tcctggcatc cactaaatat
     2281 aatggagccc gctttttaag ctggcatcca gaaaaaaaaa gaatcccagc accaaaatat
     2341 tgttttcttc accaaccatc agttcatagg tccattctct tagcgcaact acagagaaca
     2401 ggggcacaaa caggcaaaaa acgggcacaa cctcaatgga gtgatgcaac ctgcctggag
     2461 taaatgatga cacaaggcaa ttgacccacg catgtatcta tctcattttc ttacaccttc
     2521 tattaccttc tgctctctct gatttggaaa aagctgaaaa aaaaggttga aaccagttcc
     2581 ctgaaattat tcccctactt gactaataag tatataaaga cggtaggtat tgattgtaat
     2641 tctgtaaatc tatttcttaa acttcttaaa ttctactttt atagttagtc ttttttttag
     2701 ttttaaaaca ccaagaactt agtttcgaat aaacacacat aaacaaacaa aagatctatg
     2761 gtgtccaaag gagaggagtt aatcaaggaa aacatgagaa tgaaagttgt catggagggc
     2821 tccgttaatg gtcaccaatt caagtgtaca ggggaaggtg aaggtaatcc ttacatgggt
     2881 acacaaacta tgagaattaa agtaattgaa ggcggaccac taccatttgc atttgacatt
     2941 ctggcaacgt cattcatgta cggatcacga actttcatca agtaccctaa aggtatacca
     3001 gactttttca agcaatcttt tccagagggt tttacatggg aaagggttac aagatacgaa
     3061 gatgggggtg tcgtcacagt tatgcaagat acttcattag aagatggctg ccttgtctat
     3121 catgtgcaag taagaggggt gaattttcct tctaacggac ctgtgatgca gaaaaagacc
     3181 aaaggttggg aaccaaatac tgaaatgatg tacccagctg atggaggttt gagaggctac
     3241 acacacatgg cgcttaaagt tgatggtgga ggtcatttgt cttgtagttt tgttaccact
     3301 tatcgttcta aaaagactgt tggcaatatc aaaatgccag gaatacatgc tgtagaccac
     3361 agactagaaa gactcgaaga gagcgataac gaaatgttcg ttgtacagag agagcatgcc
     3421 gtagccaaat ttgctggctt aggcggtggt atggatgaat tgtataaggg atcctaactc
     3481 gaggcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     3541 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
     3601 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
     3661 acctctaccg gcatgccgag caaatgcctg caaatcgctc cccatttcgc tgagcatgag
     3721 acggaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat cataccgatg
     3781 actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg aacaccactg
     3841 catatatggc atatcacaac agtccaacta gtgcactg
//
