LOCUS       sequence-247356-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pWEAK-mRUBY2 
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0649
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     330..349
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     414..433
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      647..1235
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1136..1155
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2188..2601
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             2651..2671
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2899..2918
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2899..2917
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2942..3451
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4971..4991
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5274..6767
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7753..8463
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8479..8666
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8534..8558)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8929..9732
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10374..10767,1..422))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10374..10767,1..422))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
       61 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
      121 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
      181 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
      241 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
      301 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
      361 gcatccatgt tggaatttaa tcgcggcctg gagcaagacg tttcccgttg aatatggctc
      421 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
      481 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
      541 aatcgaactt ttgctgagtt gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt
      601 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
      661 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
      721 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
      781 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
      841 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
      901 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
      961 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     1021 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     1081 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     1141 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     1201 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     1261 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     1321 ctgtggataa ccgtgcggcc gccaatgcta aattcgagtg aaacacagga agatcagaaa
     1381 atcctcattt catccatatt aacaataatt tcaaatgttt atttgcatta tttgaaacta
     1441 ggcaagacaa gcaacgaaac gtttttgaaa attttgagta ttttcaataa atttgtagag
     1501 gactcagata ttgaaaaaaa gctacagcaa ttaatacttg ataagaagag tattgagaag
     1561 ggcaacggtt catcatctca tggatctgca catgaacaaa caccagagtc aaacgacgtt
     1621 gaaattgagg ctactgcgcc aattgatgac aatacagacg atgataacaa accgaagtta
     1681 tctgatgtag aaaaggatta aagatgctaa gagatagtga tgatatttca taaataatgt
     1741 aattctatat atgttaatta ccttttttgc gaggcatatt tatggtgaag gataagtttt
     1801 gaccatcaaa gaaggttaat gtggctgtgg tttcagggtc cataaagccc acatggataa
     1861 cattacccct gaattcgcat ctagactgaa ctggccgata attgcagacg aacgccttgc
     1921 caacagggag ttcttcagag acatggaggc tcaaaacgaa attattgaca gcctagacat
     1981 caatagtcat acaacagaaa gcgaccaccc aactttggct gataatagcg tataaacaat
     2041 gcatactttg tacgttcaaa atacaatgca gtagatatat ttatgcatat tacatataat
     2101 acatatcaca taggaagcaa caggcgcgtt ggacttttaa ttttcgagga ccgcgaatcc
     2161 ttacatcaca cccaatcccc cacaagtgat cccccacaca ccatagcttc aaaatgtttc
     2221 tactcctttt ttactcttcc agattttctc ggactccgcg catcgccgta ccacttcaaa
     2281 acacccaagc acagcatact aaatttcccc tctttcttcc tctagggtgt cgttaattac
     2341 ccgtactaaa ggtttggaaa agaaaaaaga caccgcctcg tttctttttc ttcgtcgaaa
     2401 aaggcaataa aaatttttat cacgtttctt tttcttgaaa attttttttt ttgatttttt
     2461 tctctttcga tgacctccca ttgatattta agttaataaa cggtcatcaa tttctcaagt
     2521 ttcagtttca tttttcttgt tctattacaa ctttttttac ttcttgctca ttagaaagaa
     2581 agcatagcaa tctaatctaa gttttaatta caaaagatct atggataaag cggaattaat
     2641 tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcct cggagctcca
     2701 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     2761 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     2821 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     2881 tgacgagtac ggtgggttta atacgactca ctatagggcg agcgccgaag ctagcgccac
     2941 catgaatgga gctataggag gtgacctttt gctcaatttt cctgacatgt cggtcctaga
     3001 gcgccaaagg gctcacctca agtacctcaa tcccaccttt gattctcctc tcgccggctt
     3061 ctttgccgat tcttcaatga ttaccggcgg cgagatggac agctatcttt cgactgccgg
     3121 tttgaatctt ccgatgatgt acggtgaaac gacggtggaa ggtgattcaa gactctcaat
     3181 ttcgccggaa acgacgcttg ggactggaaa tttcaagaaa cggaagtttg atacagagac
     3241 taaggattgt aatgagaaga agaagaagat gacgatgaac agagatgacc tagtagaaga
     3301 aggagaagaa gagaagtcga aaataacaga gcaaaacaat gggagcacaa aaagcatcaa
     3361 gaagatgaaa cacaaagcca agaaagaaga gaacaatttc tctaatgatt catctaaagt
     3421 gacgaaggaa ttggagaaaa cggattatat tcatgttcgt gcacgacgag gccaagccac
     3481 tgatagtcac agcatagcag aacgagttag aagagaaaag atcagtgaga gaatgaagtt
     3541 tctacaagat ttggttcctg gatgcgacaa gatcacaggc aaagcaggga tgcttgatga
     3601 aatcattaac tatgttcagt ctcttcagag acaaatcgag ttcttatcga tgaaactagc
     3661 aattgtgaat ccaaggccgg attttgatat ggatgacatt tttgccaaag aggttgcctc
     3721 aactccaatg actgtggtgc catctcctga aatggttctt tccggttatt ctcatgagat
     3781 ggttcactct ggttattcta gtgagatggt taactccggt taccttcatg tcaatccaat
     3841 gcagcaagtg aataccagtt ctgatccatt gtcatgcttc aacaatggcg aagctccttc
     3901 gatgtgggac tctcatgtgc agaatctcta tggcaattta ggagtaccgg tcatcgagct
     3961 cgagctgcag atgaatcgta gatacggatc ctaactcgag agcttttgat taagccttct
     4021 agtccaaaaa acacgttttt ttgtcattta tttcattttc ttagaatagt ttagtttatt
     4081 cattttatag tcacgaatgt tttatgattc tatatagggt tgcaaacaag catttttcat
     4141 tttatgttaa aacaatttca ggtttacctt ttattctgct tgtggtgacg cgtgtatccg
     4201 cccgctcttt tggtcaccca tgtatgctgc caaaccagat gtcaacacag ctacaacgaa
     4261 gaggatgtcc aatatttttt ttaaggaata aggatacttc aagactagat tcccccctgc
     4321 attcccatca gaaccgtaaa ccttggcgct ttccttggga agtattcaag aagtgccttg
     4381 tccggtttct gtggctcaca aaccagcgcg cccgatatgg ctttcttttc acttatgaat
     4441 gtaccagtac gggacaatta gaacgctcct gtaacaatct ctttgcaaat gtggggttac
     4501 attctaacca tgtcacactg ctgacgaaat tcaaagtaaa aaaaaatggg accacgtctt
     4561 gagaacgata gattttcttt attttacatt gaacagtcgt tgtctcagcg cgctttatgt
     4621 tttcattcat acttcatatt ataaaataac aaaagaagaa tttcatattc acgcccaaga
     4681 aatcaggctg ctttccaaat gcaattgaca cttcattagc catcacacaa aactctttct
     4741 tgctggagct tcttttaaaa aagacctcag tacaccaaac acgttacccg acctcgttat
     4801 tttacgacaa ctatgataaa attctgaaga aaaaataaaa aaattttcat acttcttgct
     4861 tttatttaaa ccattgaatg atttcttttg aacaaaacta cctgtttcac caaaggaaat
     4921 agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag atctatggcc cccaagaaga
     4981 agaggaaggt tggtattcac gggggtaccc gcccatatgc ttgccctgtc gagtcctgcg
     5041 atcgccgctt ttctcgctcg gatgagctta cccgccatat ccgcatccat accggtcaga
     5101 agcccttcca gtgtcgaatc tgcatgcgta acttcagtcg tagtgaccac cttaccaccc
     5161 acatccgcac ccacacaggc gagaagcctt ttgcctgtga catttgtggg aggaagtttg
     5221 ccaggagtga tgaacgcaag aggcatacca aaatccatac aggtgctagc ttcatgaaga
     5281 tggacaaaaa gactatagtt tggtttagaa gagatctaag gattgaggat aatcctgcat
     5341 tagcagcagc tgctcacgaa ggatctgttt ttcctgtctt catttggtgt cctgaagaag
     5401 aaggacagtt ttatcctgga agagcttcaa gatggtggat gaaacaatca cttgctcact
     5461 tatctcaatc cttgaaggct cttggatctg acctcacttt aatcaaaacc cacaacacga
     5521 tttcagcgat cttggattgt atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc
     5581 tctatgatcc tgtttcgtta gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg
     5641 ggatctctgt gcaaagctac aatggagatc tattgtatga accgtgggag atatactgcg
     5701 aaaagggcaa accttttacg agtttcaatt cttactggaa gaaatgctta gatatgtcga
     5761 ttgaatccgt tatgcttcct cctccttggc ggttgatgcc aataactgca gcggctgaag
     5821 cgatttgggc gtgttcgatt gaagaactag ggctggagaa tgaggccgag aaaccgagca
     5881 atgcgttgtt aactagagct tggagtccag gatggagcaa tgctgataag ttactaaatg
     5941 agttcatcga gaagcagttg atagattatg caaagaacag caagaaagtt gttgggaatt
     6001 ctacttcact actttctccg tatctccatt tcggggaaat aagcgtcaga cacgttttcc
     6061 agtgtgcccg gatgaaacaa attatatggg caagagataa gaacagtgaa ggagaagaaa
     6121 gtgcagatct ttttcttagg ggaatcggtt taagagagta ttctcggtat atatgtttca
     6181 acttcccgtt tactcacgag caatcgttgt tgagtcatct tcggtttttc ccttgggatg
     6241 ctgatgttga taagttcaag gcctggagac aaggcaggac cggttatccg ttggtggatg
     6301 ccggaatgag agagctttgg gctaccggat ggatgcataa cagaataaga gtgattgttt
     6361 caagctttgc tgtgaagttt cttctccttc catggaaatg gggaatgaag tatttctggg
     6421 atacactttt ggatgctgat ttggaatgtg acatccttgg ctggcagtat atctctggga
     6481 gtatccccga tggccacgag cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat
     6541 atgacccaga aggtgagtac ataaggcaat ggcttcccga gcttgcgaga ttgccaactg
     6601 aatggatcca tcatccatgg gacgctcctt taaccgtact caaagcttct ggtgtggaac
     6661 tcggaacaaa ctatgcgaaa cccattgtag acatcgacac agctcgtgag ctactagcta
     6721 aagctatttc aagaacccgt gaagcacaga tcatgatcgg agcagcagcc cggggatcct
     6781 aactcgaggc caattggtgc ggcaattgat aataacgaaa atgtctttta atgatctggg
     6841 tataatgagg aattttccga acgtttttac tttatatata tatatacatg taacatatat
     6901 tctatacgct atagagaaag gaaatttttc aattaaaaaa aaaatagaga aagagtttca
     6961 cttcttgatt atcgctaaca ctaatggttg aagtactgct actttaattt tatgctggat
     7021 gcacacactg gcttaagatg acaacggtgt tgttatccga tacaaccgga tatttttctt
     7081 ttaatgagtc taaaccgtga tagcttcagg ttaatacaat caaaaaaagc tcaaatattc
     7141 ttttaatgcc gcgttcacag attccaattg aatacaacta ggtagttcat tatatgaagc
     7201 ctttgctact atttttcact atagtctgcc ttcaccttaa tgcagacatc cacatatttt
     7261 aatcacttta aaataaaaag gaagatatat tagaagctat gatccaatct gtaagccaga
     7321 ttaaaattca cgaactcttc tttcatttga attgaatgct ttgagttggg gtagattatc
     7381 gcaaattact catcacattt attgactacg aacttgctga tgtccttttt ttatttatat
     7441 ttttcttcag tgaagcgatt ttttttttac acagaccaag acggaaaaaa gtagctaagg
     7501 aagaaaacaa aatcatgaaa aaaatgtgaa gtgatcatgc acatcgcatc aacttaaaca
     7561 ttggcttaga gatatataga gttagagttt acggcaacct ttaagcacca ataccttttg
     7621 gcatagtcta aagacctggt tcttaatttt aaacaaattt aactaaagat ttccctatca
     7681 aagaagtaac gagttgacag attttctcaa aataaatcga tactgcattt ctaggcatat
     7741 ccagcgagat ctatggtgtc caaaggagag gagttaatca aggaaaacat gagaatgaaa
     7801 gttgtcatgg agggctccgt taatggtcac caattcaagt gtacagggga aggtgaaggt
     7861 aatccttaca tgggtacaca aactatgaga attaaagtaa ttgaaggcgg accactacca
     7921 tttgcatttg acattctggc aacgtcattc atgtacggat cacgaacttt catcaagtac
     7981 cctaaaggta taccagactt tttcaagcaa tcttttccag agggttttac atgggaaagg
     8041 gttacaagat acgaagatgg gggtgtcgtc acagttatgc aagatacttc attagaagat
     8101 ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt ttccttctaa cggacctgtg
     8161 atgcagaaaa agaccaaagg ttgggaacca aatactgaaa tgatgtaccc agctgatgga
     8221 ggtttgagag gctacacaca catggcgctt aaagttgatg gtggaggtca tttgtcttgt
     8281 agttttgtta ccacttatcg ttctaaaaag actgttggca atatcaaaat gccaggaata
     8341 catgctgtag accacagact agaaagactc gaagagagcg ataacgaaat gttcgttgta
     8401 cagagagagc atgccgtagc caaatttgct ggcttaggcg gtggtatgga tgaattgtat
     8461 aagggatcct aactcgaggc gaatttctta tgatttatga tttttattat taaataagtt
     8521 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
     8581 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
     8641 gctcttattg accacacctc taccggcatg ccgagcaaat gcctgcaaat cgctccccat
     8701 ttcgctgagc agttacagag atgttacgaa ccactagtgc actgcagtac acggtttcct
     8761 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     8821 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     8881 ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa gataaatcat gtcgaaagct
     8941 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     9001 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     9061 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     9121 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     9181 aattttttac tcttcgagga cagaaaattt gctgacattg gtaatacagt caaattgcag
     9241 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     9301 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     9361 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     9421 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     9481 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     9541 ggtttagatg acaagggaga tgcattgggt caacagtata gaaccgtgga tgatgtggtt
     9601 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     9661 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     9721 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     9781 gcttcaattt aattatatca gttattaccc gagtagagca cttgaatcca ctgccccggg
     9841 aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa aaattggaaa gaaaaagctt
     9901 catggccttt ataaaaagga actatccaat acctcgccag aaccaagtaa cagtatttta
     9961 cggggcacaa atcaagaaca ataagacagg actgtaaaga tggacgcatt gaactccaaa
    10021 gaacaacaag agttccaaaa agtagtggaa caaaagcaaa tgaaggattt catgcgtttg
    10081 tactctaatc tggtagaaag atgtttcaca gactgtgtca atgacttcac aacatcaaag
    10141 ctaaccaata aggaacaaac atgcatcatg aagtgctcag aaaagttctt gaagcatagc
    10201 gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct tgggacaagg cttgggccga
    10261 taaggtgtac tggcgtatat atatctaatt atgtatctct ggtgtagccc atttttagca
    10321 tgtaaatata aagaccgagc ggccgcgtgt tacaaccaat taaccaattc tgattagaaa
    10381 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
    10441 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
    10501 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
    10561 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
    10621 ggtgagaatg gcaaaagctt atgcatttct ttccagactt gttcaacagg ccagccatta
    10681 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
    10741 gcgaggcgaa atacgcgatc gctgtta
//
