LOCUS       sequence-247357-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0650
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     312..331
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     396..415
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      629..1217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1118..1137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2170..2583
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             2633..2653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2881..2900
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2881..2899
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2924..3433
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4953..4973
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5256..6749
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7735..8445
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8461..8648
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8516..8540)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8911..9714
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10356..10767,1..404))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10356..10767,1..404))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
       61 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
      121 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
      181 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
      241 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
      301 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
      361 aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
      421 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
      481 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
      541 ttgaaggatc agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
      601 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
      661 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
      721 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      781 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      841 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      901 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      961 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1021 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1081 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1141 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1201 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     1321 ccgccaatgc taaattcgag tgaaacacag gaagatcaga aaatcctcat ttcatccata
     1381 ttaacaataa tttcaaatgt ttatttgcat tatttgaaac taggcaagac aagcaacgaa
     1441 acgtttttga aaattttgag tattttcaat aaatttgtag aggactcaga tattgaaaaa
     1501 aagctacagc aattaatact tgataagaag agtattgaga agggcaacgg ttcatcatct
     1561 catggatctg cacatgaaca aacaccagag tcaaacgacg ttgaaattga ggctactgcg
     1621 ccaattgatg acaatacaga cgatgataac aaaccgaagt tatctgatgt agaaaaggat
     1681 taaagatgct aagagatagt gatgatattt cataaataat gtaattctat atatgttaat
     1741 tacctttttt gcgaggcata tttatggtga aggataagtt ttgaccatca aagaaggtta
     1801 atgtggctgt ggtttcaggg tccataaagc ccacatggat aacattaccc ctgaattcgc
     1861 atctagactg aactggccga taattgcaga cgaacgcctt gccaacaggg agttcttcag
     1921 agacatggag gctcaaaacg aaattattga cagcctagac atcaatagtc atacaacaga
     1981 aagcgaccac ccaactttgg ctgataatag cgtataaaca atgcatactt tgtacgttca
     2041 aaatacaatg cagtagatat atttatgcat attacatata atacatatca cataggaagc
     2101 aacaggcgcg ttggactttt aattttcgag gaccgcgaat ccttacatca cacccaatcc
     2161 cccacaagtg atcccccaca caccatagct tcaaaatgtt tctactcctt ttttactctt
     2221 ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa gcacagcata
     2281 ctaaatttcc cctctttctt cctctagggt gtcgttaatt acccgtacta aaggtttgga
     2341 aaagaaaaaa gacaccgcct cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt
     2401 atcacgtttc tttttcttga aaattttttt ttttgatttt tttctctttc gatgacctcc
     2461 cattgatatt taagttaata aacggtcatc aatttctcaa gtttcagttt catttttctt
     2521 gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc aatctaatct
     2581 aagttttaat tacaaaagat ctatggataa agcggaatta attcccgagc ctccaaaaaa
     2641 gaagagaaag gtcgaattgg gtaccgccgc ctcggagctc cacttagacg gcgaggacgt
     2701 ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg gggacgggga
     2761 ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc tggatatggc
     2821 cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt acggtgggtt
     2881 taatacgact cactataggg cgagcgccga agctagcgcc accatgaatg gagctatagg
     2941 aggtgacctt ttgctcaatt ttcctgacat gtcggtccta gagcgccaaa gggctcacct
     3001 caagtacctc aatcccacct ttgattctcc tctcgccggc ttctttgccg attcttcaat
     3061 gattaccggc ggcgagatgg acagctatct ttcgactgcc ggtttgaatc ttccgatgat
     3121 gtacggtgaa acgacggtgg aaggtgattc aagactctca atttcgccgg aaacgacgct
     3181 tgggactgga aatttcaaga aacggaagtt tgatacagag actaaggatt gtaatgagaa
     3241 gaagaagaag atgacgatga acagagatga cctagtagaa gaaggagaag aagagaagtc
     3301 gaaaataaca gagcaaaaca atgggagcac aaaaagcatc aagaagatga aacacaaagc
     3361 caagaaagaa gagaacaatt tctctaatga ttcatctaaa gtgacgaagg aattggagaa
     3421 aacggattat attcatgttc gtgcacgacg aggccaagcc actgatagtc acagcatagc
     3481 agaacgagtt agaagagaaa agatcagtga gagaatgaag tttctacaag atttggttcc
     3541 tggatgcgac aagatcacag gcaaagcagg gatgcttgat gaaatcatta actatgttca
     3601 gtctcttcag agacaaatcg agttcttatc gatgaaacta gcaattgtga atccaaggcc
     3661 ggattttgat atggatgaca tttttgccaa agaggttgcc tcaactccaa tgactgtggt
     3721 gccatctcct gaaatggttc tttccggtta ttctcatgag atggttcact ctggttattc
     3781 tagtgagatg gttaactccg gttaccttca tgtcaatcca atgcagcaag tgaataccag
     3841 ttctgatcca ttgtcatgct tcaacaatgg cgaagctcct tcgatgtggg actctcatgt
     3901 gcagaatctc tatggcaatt taggagtacc ggtcatcgag ctcgagctgc agatgaatcg
     3961 tagatacgga tcctaactcg agagcttttg attaagcctt ctagtccaaa aaacacgttt
     4021 ttttgtcatt tatttcattt tcttagaata gtttagttta ttcattttat agtcacgaat
     4081 gttttatgat tctatatagg gttgcaaaca agcatttttc attttatgtt aaaacaattt
     4141 caggtttacc ttttattctg cttgtggtga cgcgtgtatc cgcccgctct tttggtcacc
     4201 catgtatgct gccaaaccag atgtcaacac agctacaacg aagaggatgt ccaatatttt
     4261 ttttaaggaa taaggatact tcaagactag attcccccct gcattcccat cagaaccgta
     4321 aaccttggcg ctttccttgg gaagtattca agaagtgcct tgtccggttt ctgtggctca
     4381 caaaccagcg cgcccgatat ggctttcttt tcacttatga atgtaccagt acgggacaat
     4441 tagaacgctc ctgtaacaat ctctttgcaa atgtggggtt acattctaac catgtcacac
     4501 tgctgacgaa attcaaagta aaaaaaaatg ggaccacgtc ttgagaacga tagattttct
     4561 ttattttaca ttgaacagtc gttgtctcag cgcgctttat gttttcattc atacttcata
     4621 ttataaaata acaaaagaag aatttcatat tcacgcccaa gaaatcaggc tgctttccaa
     4681 atgcaattga cacttcatta gccatcacac aaaactcttt cttgctggag cttcttttaa
     4741 aaaagacctc agtacaccaa acacgttacc cgacctcgtt attttacgac aactatgata
     4801 aaattctgaa gaaaaaataa aaaaattttc atacttcttg cttttattta aaccattgaa
     4861 tgatttcttt tgaacaaaac tacctgtttc accaaaggaa atagaaagaa aaaatcaatt
     4921 agaagaaaac aaaaaacaaa agatctatgg cccccaagaa gaagaggaag gttggtattc
     4981 acgggggtac ccgcccatat gcttgccctg tcgagtcctg cgatcgccgc ttttctcgct
     5041 cggatgagct tacccgccat atccgcatcc ataccggtca gaagcccttc cagtgtcgaa
     5101 tctgcatgcg taacttcagt cgtagtgacc accttaccac ccacatccgc acccacacag
     5161 gcgagaagcc ttttgcctgt gacatttgtg ggaggaagtt tgccaggagt gatgaacgca
     5221 agaggcatac caaaatccat acaggtgcta gcttcatgaa gatggacaaa aagactatag
     5281 tttggtttag aagagatcta aggattgagg ataatcctgc attagcagca gctgctcacg
     5341 aaggatctgt ttttcctgtc ttcatttggt gtcctgaaga agaaggacag ttttatcctg
     5401 gaagagcttc aagatggtgg atgaaacaat cacttgctca cttatctcaa tccttgaagg
     5461 ctcttggatc tgacctcact ttaatcaaaa cccacaacac gatttcagcg atcttggatt
     5521 gtatccgcgt taccggtgct acaaaagtcg tctttaacca cctctatgat cctgtttcgt
     5581 tagttcggga ccataccgta aaggagaagc tggtggaacg tgggatctct gtgcaaagct
     5641 acaatggaga tctattgtat gaaccgtggg agatatactg cgaaaagggc aaacctttta
     5701 cgagtttcaa ttcttactgg aagaaatgct tagatatgtc gattgaatcc gttatgcttc
     5761 ctcctccttg gcggttgatg ccaataactg cagcggctga agcgatttgg gcgtgttcga
     5821 ttgaagaact agggctggag aatgaggccg agaaaccgag caatgcgttg ttaactagag
     5881 cttggagtcc aggatggagc aatgctgata agttactaaa tgagttcatc gagaagcagt
     5941 tgatagatta tgcaaagaac agcaagaaag ttgttgggaa ttctacttca ctactttctc
     6001 cgtatctcca tttcggggaa ataagcgtca gacacgtttt ccagtgtgcc cggatgaaac
     6061 aaattatatg ggcaagagat aagaacagtg aaggagaaga aagtgcagat ctttttctta
     6121 ggggaatcgg tttaagagag tattctcggt atatatgttt caacttcccg tttactcacg
     6181 agcaatcgtt gttgagtcat cttcggtttt tcccttggga tgctgatgtt gataagttca
     6241 aggcctggag acaaggcagg accggttatc cgttggtgga tgccggaatg agagagcttt
     6301 gggctaccgg atggatgcat aacagaataa gagtgattgt ttcaagcttt gctgtgaagt
     6361 ttcttctcct tccatggaaa tggggaatga agtatttctg ggatacactt ttggatgctg
     6421 atttggaatg tgacatcctt ggctggcagt atatctctgg gagtatcccc gatggccacg
     6481 agcttgatcg cttggacaat cccgcgttac aaggcgccaa atatgaccca gaaggtgagt
     6541 acataaggca atggcttccc gagcttgcga gattgccaac tgaatggatc catcatccat
     6601 gggacgctcc tttaaccgta ctcaaagctt ctggtgtgga actcggaaca aactatgcga
     6661 aacccattgt agacatcgac acagctcgtg agctactagc taaagctatt tcaagaaccc
     6721 gtgaagcaca gatcatgatc ggagcagcag cccggggatc ctaactcgag gccaattggt
     6781 gcggcaattg ataataacga aaatgtcttt taatgatctg ggtataatga ggaattttcc
     6841 gaacgttttt actttatata tatatataca tgtaacatat attctatacg ctatagagaa
     6901 aggaaatttt tcaattaaaa aaaaaataga gaaagagttt cacttcttga ttatcgctaa
     6961 cactaatggt tgaagtactg ctactttaat tttatgctgg atgcacacac tggcttaaga
     7021 tgacaacgaa gaggatgtcc aatatttttt ttaaggaata aggatacttc aagactagat
     7081 tcccccctgc attcccatca gaaccgtaaa ccttggcgct ttccttggga agtattcaag
     7141 aagtgccttg tccggtttct gtggctcaca aaccagcgcg cccgatatgg ctttcttttc
     7201 acttatgaat gtaccagtac gggacaatta gaacgctcct gtaacaatct ctttgcaaat
     7261 gtggggttac attctaacca tgtcacactg ctgacgaaat tcaaagtaaa aaaaaatggg
     7321 accacgtctt gagaacgata gattttcttt attttacatt gaacagtcgt tgtctcagcg
     7381 cgctttatgt tttcattcat acttcatatt ataaaataac aaaagaagaa tttcatattc
     7441 acgcccaaga aatcaggctg ctttccaaat gcaattgaca cttcattagc catcacacaa
     7501 aactctttct tgctggagct tcttttaaaa aagacctcag tacaccaaac acgttacccg
     7561 acctcgttat tttacgacaa ctatgataaa attctgaaga aaaaataaaa aaattttcat
     7621 acttcttgct tttatttaaa ccattgaatg atttcttttg aacaaaacta cctgtttcac
     7681 caaaggaaat agaaagaaaa aatcaattag aagaaaacaa aaaacaaaag atctatggtg
     7741 tccaaaggag aggagttaat caaggaaaac atgagaatga aagttgtcat ggagggctcc
     7801 gttaatggtc accaattcaa gtgtacaggg gaaggtgaag gtaatcctta catgggtaca
     7861 caaactatga gaattaaagt aattgaaggc ggaccactac catttgcatt tgacattctg
     7921 gcaacgtcat tcatgtacgg atcacgaact ttcatcaagt accctaaagg tataccagac
     7981 tttttcaagc aatcttttcc agagggtttt acatgggaaa gggttacaag atacgaagat
     8041 gggggtgtcg tcacagttat gcaagatact tcattagaag atggctgcct tgtctatcat
     8101 gtgcaagtaa gaggggtgaa ttttccttct aacggacctg tgatgcagaa aaagaccaaa
     8161 ggttgggaac caaatactga aatgatgtac ccagctgatg gaggtttgag aggctacaca
     8221 cacatggcgc ttaaagttga tggtggaggt catttgtctt gtagttttgt taccacttat
     8281 cgttctaaaa agactgttgg caatatcaaa atgccaggaa tacatgctgt agaccacaga
     8341 ctagaaagac tcgaagagag cgataacgaa atgttcgttg tacagagaga gcatgccgta
     8401 gccaaatttg ctggcttagg cggtggtatg gatgaattgt ataagggatc ctaactcgag
     8461 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta
     8521 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttattctt gagtaactct
     8581 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc
     8641 tctaccggca tgccgagcaa atgcctgcaa atcgctcccc atttcgctga gcagttacag
     8701 agatgttacg aaccactagt gcactgcagt acacggtttc cttgaaattt ttttgattcg
     8761 gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
     8821 cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
     8881 acaaaaacgt gcaggaaacg aagataaatc atgtcgaaag ctacatataa ggaacgtgct
     8941 gctactcatc ctagtcctgt tgctgccaag ctatttaata tcatgcacga aaagcaaaca
     9001 aacttgtgtg cttcattgga tgttcgtacc accaaggaat tactggagtt agttgaagca
     9061 ttaggtccca aaatttgttt actaaaaaca catgtggata tcttgactga tttttccatg
     9121 gagggcacag ttaagccgct aaaggcatta tccgccaagt acaatttttt actcttcgag
     9181 gacagaaaat ttgctgacat tggtaataca gtcaaattgc agtactctgc gggtgtatac
     9241 agaatagcag aatgggcaga cattacgaat gcacacggtg tggtgggccc aggtattgtt
     9301 agcggtttga agcaggcggc agaagaagta acaaaggaac ctagaggcct tttgatgtta
     9361 gcagaattgt catgcaaggg ctccctatct actggagaat atactaaggg tactgttgac
     9421 attgcgaaga gcgacaaaga ttttgttatc ggctttattg ctcaaagaga catgggtgga
     9481 agagatgaag gttacgattg gttgattatg acacccggtg tgggtttaga tgacaaggga
     9541 gatgcattgg gtcaacagta tagaaccgtg gatgatgtgg tttctacagg atctgacatt
     9601 attattgttg gaagaggact atttgcaaag ggaagggatg ctaaggtaga gggtgaacgt
     9661 tacagaaaag caggctggga agcatatttg agaagatgcg gccagcaaaa ctaaaaaact
     9721 gtattataag taaatgcatg tatactaaac tcacaaatta gagcttcaat ttaattatat
     9781 cagttattac ccgagtagag cacttgaatc cactgccccg ggaatctcgg tcgtaatgat
     9841 ttctataatg acgaaaaaaa aaaaattgga aagaaaaagc ttcatggcct ttataaaaag
     9901 gaactatcca atacctcgcc agaaccaagt aacagtattt tacggggcac aaatcaagaa
     9961 caataagaca ggactgtaaa gatggacgca ttgaactcca aagaacaaca agagttccaa
    10021 aaagtagtgg aacaaaagca aatgaaggat ttcatgcgtt tgtactctaa tctggtagaa
    10081 agatgtttca cagactgtgt caatgacttc acaacatcaa agctaaccaa taaggaacaa
    10141 acatgcatca tgaagtgctc agaaaagttc ttgaagcata gcgaacgtgt agggcagcgt
    10201 ttccaagaac aaaacgctgc cttgggacaa ggcttgggcc gataaggtgt actggcgtat
    10261 atatatctaa ttatgtatct ctggtgtagc ccatttttag catgtaaata taaagaccga
    10321 gcggccgcgt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
    10381 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
    10441 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
    10501 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
    10561 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
    10621 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
    10681 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgaggcg aaatacgcga
    10741 tcgctgttaa aaggacaatt acaaaca
//
