LOCUS       sequence-247358-       10747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0651
FEATURES             Location/Qualifiers
     source          1..10747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     310..329
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     394..413
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      627..1215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1116..1135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2168..2581
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             2631..2651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2879..2898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2879..2897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2922..3431
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4951..4971
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5254..6747
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     promoter        7040..7706
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7576..7599
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             7713..8423
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8439..8626
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8494..8518)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8889..9692
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10334..10747,1..402))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10334..10747,1..402))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
       61 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
      121 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
      181 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
      241 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
      301 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
      361 tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
      421 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
      481 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
      541 gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
      601 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      661 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      721 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      781 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      841 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      901 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      961 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1021 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1081 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1141 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1201 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1261 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc
     1321 gccaatgcta aattcgagtg aaacacagga agatcagaaa atcctcattt catccatatt
     1381 aacaataatt tcaaatgttt atttgcatta tttgaaacta ggcaagacaa gcaacgaaac
     1441 gtttttgaaa attttgagta ttttcaataa atttgtagag gactcagata ttgaaaaaaa
     1501 gctacagcaa ttaatacttg ataagaagag tattgagaag ggcaacggtt catcatctca
     1561 tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg ctactgcgcc
     1621 aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag aaaaggatta
     1681 aagatgctaa gagatagtga tgatatttca taaataatgt aattctatat atgttaatta
     1741 ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa gaaggttaat
     1801 gtggctgtgg tttcagggtc cataaagccc acatggataa cattacccct gaattcgcat
     1861 ctagactgaa ctggccgata attgcagacg aacgccttgc caacagggag ttcttcagag
     1921 acatggaggc tcaaaacgaa attattgaca gcctagacat caatagtcat acaacagaaa
     1981 gcgaccaccc aactttggct gataatagcg tataaacaat gcatactttg tacgttcaaa
     2041 atacaatgca gtagatatat ttatgcatat tacatataat acatatcaca taggaagcaa
     2101 caggcgcgtt ggacttttaa ttttcgagga ccgcgaatcc ttacatcaca cccaatcccc
     2161 cacaagtgat cccccacaca ccatagcttc aaaatgtttc tactcctttt ttactcttcc
     2221 agattttctc ggactccgcg catcgccgta ccacttcaaa acacccaagc acagcatact
     2281 aaatttcccc tctttcttcc tctagggtgt cgttaattac ccgtactaaa ggtttggaaa
     2341 agaaaaaaga caccgcctcg tttctttttc ttcgtcgaaa aaggcaataa aaatttttat
     2401 cacgtttctt tttcttgaaa attttttttt ttgatttttt tctctttcga tgacctccca
     2461 ttgatattta agttaataaa cggtcatcaa tttctcaagt ttcagtttca tttttcttgt
     2521 tctattacaa ctttttttac ttcttgctca ttagaaagaa agcatagcaa tctaatctaa
     2581 gttttaatta caaaagatct atggataaag cggaattaat tcccgagcct ccaaaaaaga
     2641 agagaaaggt cgaattgggt accgccgcct cggagctcca cttagacggc gaggacgtgg
     2701 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
     2761 ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
     2821 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggttta
     2881 atacgactca ctatagggcg agcgccgaag ctagcgccac catgaatgga gctataggag
     2941 gtgacctttt gctcaatttt cctgacatgt cggtcctaga gcgccaaagg gctcacctca
     3001 agtacctcaa tcccaccttt gattctcctc tcgccggctt ctttgccgat tcttcaatga
     3061 ttaccggcgg cgagatggac agctatcttt cgactgccgg tttgaatctt ccgatgatgt
     3121 acggtgaaac gacggtggaa ggtgattcaa gactctcaat ttcgccggaa acgacgcttg
     3181 ggactggaaa tttcaagaaa cggaagtttg atacagagac taaggattgt aatgagaaga
     3241 agaagaagat gacgatgaac agagatgacc tagtagaaga aggagaagaa gagaagtcga
     3301 aaataacaga gcaaaacaat gggagcacaa aaagcatcaa gaagatgaaa cacaaagcca
     3361 agaaagaaga gaacaatttc tctaatgatt catctaaagt gacgaaggaa ttggagaaaa
     3421 cggattatat tcatgttcgt gcacgacgag gccaagccac tgatagtcac agcatagcag
     3481 aacgagttag aagagaaaag atcagtgaga gaatgaagtt tctacaagat ttggttcctg
     3541 gatgcgacaa gatcacaggc aaagcaggga tgcttgatga aatcattaac tatgttcagt
     3601 ctcttcagag acaaatcgag ttcttatcga tgaaactagc aattgtgaat ccaaggccgg
     3661 attttgatat ggatgacatt tttgccaaag aggttgcctc aactccaatg actgtggtgc
     3721 catctcctga aatggttctt tccggttatt ctcatgagat ggttcactct ggttattcta
     3781 gtgagatggt taactccggt taccttcatg tcaatccaat gcagcaagtg aataccagtt
     3841 ctgatccatt gtcatgcttc aacaatggcg aagctccttc gatgtgggac tctcatgtgc
     3901 agaatctcta tggcaattta ggagtaccgg tcatcgagct cgagctgcag atgaatcgta
     3961 gatacggatc ctaactcgag agcttttgat taagccttct agtccaaaaa acacgttttt
     4021 ttgtcattta tttcattttc ttagaatagt ttagtttatt cattttatag tcacgaatgt
     4081 tttatgattc tatatagggt tgcaaacaag catttttcat tttatgttaa aacaatttca
     4141 ggtttacctt ttattctgct tgtggtgacg cgtgtatccg cccgctcttt tggtcaccca
     4201 tgtatgctgc caaaccagat gtcaacacag ctacaacgaa gaggatgtcc aatatttttt
     4261 ttaaggaata aggatacttc aagactagat tcccccctgc attcccatca gaaccgtaaa
     4321 ccttggcgct ttccttggga agtattcaag aagtgccttg tccggtttct gtggctcaca
     4381 aaccagcgcg cccgatatgg ctttcttttc acttatgaat gtaccagtac gggacaatta
     4441 gaacgctcct gtaacaatct ctttgcaaat gtggggttac attctaacca tgtcacactg
     4501 ctgacgaaat tcaaagtaaa aaaaaatggg accacgtctt gagaacgata gattttcttt
     4561 attttacatt gaacagtcgt tgtctcagcg cgctttatgt tttcattcat acttcatatt
     4621 ataaaataac aaaagaagaa tttcatattc acgcccaaga aatcaggctg ctttccaaat
     4681 gcaattgaca cttcattagc catcacacaa aactctttct tgctggagct tcttttaaaa
     4741 aagacctcag tacaccaaac acgttacccg acctcgttat tttacgacaa ctatgataaa
     4801 attctgaaga aaaaataaaa aaattttcat acttcttgct tttatttaaa ccattgaatg
     4861 atttcttttg aacaaaacta cctgtttcac caaaggaaat agaaagaaaa aatcaattag
     4921 aagaaaacaa aaaacaaaag atctatggcc cccaagaaga agaggaaggt tggtattcac
     4981 gggggtaccc gcccatatgc ttgccctgtc gagtcctgcg atcgccgctt ttctcgctcg
     5041 gatgagctta cccgccatat ccgcatccat accggtcaga agcccttcca gtgtcgaatc
     5101 tgcatgcgta acttcagtcg tagtgaccac cttaccaccc acatccgcac ccacacaggc
     5161 gagaagcctt ttgcctgtga catttgtggg aggaagtttg ccaggagtga tgaacgcaag
     5221 aggcatacca aaatccatac aggtgctagc ttcatgaaga tggacaaaaa gactatagtt
     5281 tggtttagaa gagatctaag gattgaggat aatcctgcat tagcagcagc tgctcacgaa
     5341 ggatctgttt ttcctgtctt catttggtgt cctgaagaag aaggacagtt ttatcctgga
     5401 agagcttcaa gatggtggat gaaacaatca cttgctcact tatctcaatc cttgaaggct
     5461 cttggatctg acctcacttt aatcaaaacc cacaacacga tttcagcgat cttggattgt
     5521 atccgcgtta ccggtgctac aaaagtcgtc tttaaccacc tctatgatcc tgtttcgtta
     5581 gttcgggacc ataccgtaaa ggagaagctg gtggaacgtg ggatctctgt gcaaagctac
     5641 aatggagatc tattgtatga accgtgggag atatactgcg aaaagggcaa accttttacg
     5701 agtttcaatt cttactggaa gaaatgctta gatatgtcga ttgaatccgt tatgcttcct
     5761 cctccttggc ggttgatgcc aataactgca gcggctgaag cgatttgggc gtgttcgatt
     5821 gaagaactag ggctggagaa tgaggccgag aaaccgagca atgcgttgtt aactagagct
     5881 tggagtccag gatggagcaa tgctgataag ttactaaatg agttcatcga gaagcagttg
     5941 atagattatg caaagaacag caagaaagtt gttgggaatt ctacttcact actttctccg
     6001 tatctccatt tcggggaaat aagcgtcaga cacgttttcc agtgtgcccg gatgaaacaa
     6061 attatatggg caagagataa gaacagtgaa ggagaagaaa gtgcagatct ttttcttagg
     6121 ggaatcggtt taagagagta ttctcggtat atatgtttca acttcccgtt tactcacgag
     6181 caatcgttgt tgagtcatct tcggtttttc ccttgggatg ctgatgttga taagttcaag
     6241 gcctggagac aaggcaggac cggttatccg ttggtggatg ccggaatgag agagctttgg
     6301 gctaccggat ggatgcataa cagaataaga gtgattgttt caagctttgc tgtgaagttt
     6361 cttctccttc catggaaatg gggaatgaag tatttctggg atacactttt ggatgctgat
     6421 ttggaatgtg acatccttgg ctggcagtat atctctggga gtatccccga tggccacgag
     6481 cttgatcgct tggacaatcc cgcgttacaa ggcgccaaat atgacccaga aggtgagtac
     6541 ataaggcaat ggcttcccga gcttgcgaga ttgccaactg aatggatcca tcatccatgg
     6601 gacgctcctt taaccgtact caaagcttct ggtgtggaac tcggaacaaa ctatgcgaaa
     6661 cccattgtag acatcgacac agctcgtgag ctactagcta aagctatttc aagaacccgt
     6721 gaagcacaga tcatgatcgg agcagcagcc cggggatcct aactcgaggc caattggtgc
     6781 ggcaattgat aataacgaaa atgtctttta atgatctggg tataatgagg aattttccga
     6841 acgtttttac tttatatata tatatacatg taacatatat tctatacgct atagagaaag
     6901 gaaatttttc aattaaaaaa aaaatagaga aagagtttca cttcttgatt atcgctaaca
     6961 ctaatggttg aagtactgct actttaattt tatgctggat gcacacactg gcttaagatg
     7021 acaacgcagt tcgagtttat cattatcaat actgccattt caaagaatac gtaaataatt
     7081 aatagtagtg attttcctaa ctttatttag tcaaaaaatt agccttttaa ttctgctgta
     7141 acccgtacat gcccaaaata gggggcgggt tacacagaat atataacatc gtaggtgtct
     7201 gggtgaacag tttattcctg gcatccacta aatataatgg agcccgcttt ttaagctggc
     7261 atccagaaaa aaaaagaatc ccagcaccaa aatattgttt tcttcaccaa ccatcagttc
     7321 ataggtccat tctcttagcg caactacaga gaacaggggc acaaacaggc aaaaaacggg
     7381 cacaacctca atggagtgat gcaacctgcc tggagtaaat gatgacacaa ggcaattgac
     7441 ccacgcatgt atctatctca ttttcttaca ccttctatta ccttctgctc tctctgattt
     7501 ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa attattcccc tacttgacta
     7561 ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt cttaaacttc
     7621 ttaaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag aacttagttt
     7681 cgaataaaca cacataaaca aacaaaagat ctatggtgtc caaaggagag gagttaatca
     7741 aggaaaacat gagaatgaaa gttgtcatgg agggctccgt taatggtcac caattcaagt
     7801 gtacagggga aggtgaaggt aatccttaca tgggtacaca aactatgaga attaaagtaa
     7861 ttgaaggcgg accactacca tttgcatttg acattctggc aacgtcattc atgtacggat
     7921 cacgaacttt catcaagtac cctaaaggta taccagactt tttcaagcaa tcttttccag
     7981 agggttttac atgggaaagg gttacaagat acgaagatgg gggtgtcgtc acagttatgc
     8041 aagatacttc attagaagat ggctgccttg tctatcatgt gcaagtaaga ggggtgaatt
     8101 ttccttctaa cggacctgtg atgcagaaaa agaccaaagg ttgggaacca aatactgaaa
     8161 tgatgtaccc agctgatgga ggtttgagag gctacacaca catggcgctt aaagttgatg
     8221 gtggaggtca tttgtcttgt agttttgtta ccacttatcg ttctaaaaag actgttggca
     8281 atatcaaaat gccaggaata catgctgtag accacagact agaaagactc gaagagagcg
     8341 ataacgaaat gttcgttgta cagagagagc atgccgtagc caaatttgct ggcttaggcg
     8401 gtggtatgga tgaattgtat aagggatcct aactcgaggc gaatttctta tgatttatga
     8461 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     8521 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     8581 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg ccgagcaaat
     8641 gcctgcaaat cgctccccat ttcgctgagc agttacagag atgttacgaa ccactagtgc
     8701 actgcagtac acggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag
     8761 aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa
     8821 catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacgtgc aggaaacgaa
     8881 gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg
     8941 ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg
     9001 ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac
     9061 taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa
     9121 aggcattatc cgccaagtac aattttttac tcttcgagga cagaaaattt gctgacattg
     9181 gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca
     9241 ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag
     9301 aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct
     9361 ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt
     9421 ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt
     9481 tgattatgac acccggtgtg ggtttagatg acaagggaga tgcattgggt caacagtata
     9541 gaaccgtgga tgatgtggtt tctacaggat ctgacattat tattgttgga agaggactat
     9601 ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag
     9661 catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta
     9721 tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc gagtagagca
     9781 cttgaatcca ctgccccggg aatctcggtc gtaatgattt ctataatgac gaaaaaaaaa
     9841 aaattggaaa gaaaaagctt catggccttt ataaaaagga actatccaat acctcgccag
     9901 aaccaagtaa cagtatttta cggggcacaa atcaagaaca ataagacagg actgtaaaga
     9961 tggacgcatt gaactccaaa gaacaacaag agttccaaaa agtagtggaa caaaagcaaa
    10021 tgaaggattt catgcgtttg tactctaatc tggtagaaag atgtttcaca gactgtgtca
    10081 atgacttcac aacatcaaag ctaaccaata aggaacaaac atgcatcatg aagtgctcag
    10141 aaaagttctt gaagcatagc gaacgtgtag ggcagcgttt ccaagaacaa aacgctgcct
    10201 tgggacaagg cttgggccga taaggtgtac tggcgtatat atatctaatt atgtatctct
    10261 ggtgtagccc atttttagca tgtaaatata aagaccgagc ggccgcgtgt tacaaccaat
    10321 taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
    10381 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
    10441 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
    10501 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
    10561 catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct ttccagactt
    10621 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
    10681 tcattcgtga ttgcgcctga gcgaggcgaa atacgcgatc gctgttaaaa ggacaattac
    10741 aaacagg
//
