LOCUS       sequence-247361-       10767 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pMEDIUM-mRUBY2
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0654
FEATURES             Location/Qualifiers
     source          1..10767
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..332
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     397..416
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      630..1218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1119..1138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2634..2654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2882..2901
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2882..2900
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2925..3434
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4954..4974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5257..6750
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     CDS             7736..8446
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8462..8649
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8517..8541)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8912..9715
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10357..10767,1..405))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10357..10767,1..405))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
       61 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
      121 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
      181 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
      241 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
      301 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
      361 taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
      421 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
      481 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
      541 gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg
     1321 gccgccaatg ctaaattcga gtgaaacaca ggaagatcag aaaatcctca tttcatccat
     1381 attaacaata atttcaaatg tttatttgca ttatttgaaa ctaggcaaga caagcaacga
     1441 aacgtttttg aaaattttga gtattttcaa taaatttgta gaggactcag atattgaaaa
     1501 aaagctacag caattaatac ttgataagaa gagtattgag aagggcaacg gttcatcatc
     1561 tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg aggctactgc
     1621 gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg tagaaaagga
     1681 ttaaagatgc taagagatag tgatgatatt tcataaataa tgtaattcta tatatgttaa
     1741 ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc aaagaaggtt
     1801 aatgtggctg tggtttcagg gtccataaag cccacatgga taacattacc cctgaattcg
     1861 catctagact gaactggccg ataattgcag acgaacgaag aggatgtcca atattttttt
     1921 taaggaataa ggatacttca agactagatt cccccctgca ttcccatcag aaccgtaaac
     1981 cttggcgctt tccttgggaa gtattcaaga agtgccttgt ccggtttctg tggctcacaa
     2041 accagcgcgc ccgatatggc tttcttttca cttatgaatg taccagtacg ggacaattag
     2101 aacgctcctg taacaatctc tttgcaaatg tggggttaca ttctaaccat gtcacactgc
     2161 tgacgaaatt caaagtaaaa aaaaatggga ccacgtcttg agaacgatag attttcttta
     2221 ttttacattg aacagtcgtt gtctcagcgc gctttatgtt ttcattcata cttcatatta
     2281 taaaataaca aaagaagaat ttcatattca cgcccaagaa atcaggctgc tttccaaatg
     2341 caattgacac ttcattagcc atcacacaaa actctttctt gctggagctt cttttaaaaa
     2401 agacctcagt acaccaaaca cgttacccga cctcgttatt ttacgacaac tatgataaaa
     2461 ttctgaagaa aaaataaaaa aattttcata cttcttgctt ttatttaaac cattgaatga
     2521 tttcttttga acaaaactac ctgtttcacc aaaggaaata gaaagaaaaa atcaattaga
     2581 agaaaacaaa aaacaaaaga tctatggata aagcggaatt aattcccgag cctccaaaaa
     2641 agaagagaaa ggtcgaattg ggtaccgccg cctcggagct ccacttagac ggcgaggacg
     2701 tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg
     2761 attccccggg tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg
     2821 ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggt
     2881 ttaatacgac tcactatagg gcgagcgccg aagctagcgc caccatgaat ggagctatag
     2941 gaggtgacct tttgctcaat tttcctgaca tgtcggtcct agagcgccaa agggctcacc
     3001 tcaagtacct caatcccacc tttgattctc ctctcgccgg cttctttgcc gattcttcaa
     3061 tgattaccgg cggcgagatg gacagctatc tttcgactgc cggtttgaat cttccgatga
     3121 tgtacggtga aacgacggtg gaaggtgatt caagactctc aatttcgccg gaaacgacgc
     3181 ttgggactgg aaatttcaag aaacggaagt ttgatacaga gactaaggat tgtaatgaga
     3241 agaagaagaa gatgacgatg aacagagatg acctagtaga agaaggagaa gaagagaagt
     3301 cgaaaataac agagcaaaac aatgggagca caaaaagcat caagaagatg aaacacaaag
     3361 ccaagaaaga agagaacaat ttctctaatg attcatctaa agtgacgaag gaattggaga
     3421 aaacggatta tattcatgtt cgtgcacgac gaggccaagc cactgatagt cacagcatag
     3481 cagaacgagt tagaagagaa aagatcagtg agagaatgaa gtttctacaa gatttggttc
     3541 ctggatgcga caagatcaca ggcaaagcag ggatgcttga tgaaatcatt aactatgttc
     3601 agtctcttca gagacaaatc gagttcttat cgatgaaact agcaattgtg aatccaaggc
     3661 cggattttga tatggatgac atttttgcca aagaggttgc ctcaactcca atgactgtgg
     3721 tgccatctcc tgaaatggtt ctttccggtt attctcatga gatggttcac tctggttatt
     3781 ctagtgagat ggttaactcc ggttaccttc atgtcaatcc aatgcagcaa gtgaatacca
     3841 gttctgatcc attgtcatgc ttcaacaatg gcgaagctcc ttcgatgtgg gactctcatg
     3901 tgcagaatct ctatggcaat ttaggagtac cggtcatcga gctcgagctg cagatgaatc
     3961 gtagatacgg atcctaactc gagagctttt gattaagcct tctagtccaa aaaacacgtt
     4021 tttttgtcat ttatttcatt ttcttagaat agtttagttt attcatttta tagtcacgaa
     4081 tgttttatga ttctatatag ggttgcaaac aagcattttt cattttatgt taaaacaatt
     4141 tcaggtttac cttttattct gcttgtggtg acgcgtgtat ccgcccgctc ttttggtcac
     4201 ccatgtatgc tgccaaacca gatgtcaaca cagctacaac gaagaggatg tccaatattt
     4261 tttttaagga ataaggatac ttcaagacta gattcccccc tgcattccca tcagaaccgt
     4321 aaaccttggc gctttccttg ggaagtattc aagaagtgcc ttgtccggtt tctgtggctc
     4381 acaaaccagc gcgcccgata tggctttctt ttcacttatg aatgtaccag tacgggacaa
     4441 ttagaacgct cctgtaacaa tctctttgca aatgtggggt tacattctaa ccatgtcaca
     4501 ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt cttgagaacg atagattttc
     4561 tttattttac attgaacagt cgttgtctca gcgcgcttta tgttttcatt catacttcat
     4621 attataaaat aacaaaagaa gaatttcata ttcacgccca agaaatcagg ctgctttcca
     4681 aatgcaattg acacttcatt agccatcaca caaaactctt tcttgctgga gcttctttta
     4741 aaaaagacct cagtacacca aacacgttac ccgacctcgt tattttacga caactatgat
     4801 aaaattctga agaaaaaata aaaaaatttt catacttctt gcttttattt aaaccattga
     4861 atgatttctt ttgaacaaaa ctacctgttt caccaaagga aatagaaaga aaaaatcaat
     4921 tagaagaaaa caaaaaacaa aagatctatg gcccccaaga agaagaggaa ggttggtatt
     4981 cacgggggta cccgcccata tgcttgccct gtcgagtcct gcgatcgccg cttttctcgc
     5041 tcggatgagc ttacccgcca tatccgcatc cataccggtc agaagccctt ccagtgtcga
     5101 atctgcatgc gtaacttcag tcgtagtgac caccttacca cccacatccg cacccacaca
     5161 ggcgagaagc cttttgcctg tgacatttgt gggaggaagt ttgccaggag tgatgaacgc
     5221 aagaggcata ccaaaatcca tacaggtgct agcttcatga agatggacaa aaagactata
     5281 gtttggttta gaagagatct aaggattgag gataatcctg cattagcagc agctgctcac
     5341 gaaggatctg tttttcctgt cttcatttgg tgtcctgaag aagaaggaca gttttatcct
     5401 ggaagagctt caagatggtg gatgaaacaa tcacttgctc acttatctca atccttgaag
     5461 gctcttggat ctgacctcac tttaatcaaa acccacaaca cgatttcagc gatcttggat
     5521 tgtatccgcg ttaccggtgc tacaaaagtc gtctttaacc acctctatga tcctgtttcg
     5581 ttagttcggg accataccgt aaaggagaag ctggtggaac gtgggatctc tgtgcaaagc
     5641 tacaatggag atctattgta tgaaccgtgg gagatatact gcgaaaaggg caaacctttt
     5701 acgagtttca attcttactg gaagaaatgc ttagatatgt cgattgaatc cgttatgctt
     5761 cctcctcctt ggcggttgat gccaataact gcagcggctg aagcgatttg ggcgtgttcg
     5821 attgaagaac tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt gttaactaga
     5881 gcttggagtc caggatggag caatgctgat aagttactaa atgagttcat cgagaagcag
     5941 ttgatagatt atgcaaagaa cagcaagaaa gttgttggga attctacttc actactttct
     6001 ccgtatctcc atttcgggga aataagcgtc agacacgttt tccagtgtgc ccggatgaaa
     6061 caaattatat gggcaagaga taagaacagt gaaggagaag aaagtgcaga tctttttctt
     6121 aggggaatcg gtttaagaga gtattctcgg tatatatgtt tcaacttccc gtttactcac
     6181 gagcaatcgt tgttgagtca tcttcggttt ttcccttggg atgctgatgt tgataagttc
     6241 aaggcctgga gacaaggcag gaccggttat ccgttggtgg atgccggaat gagagagctt
     6301 tgggctaccg gatggatgca taacagaata agagtgattg tttcaagctt tgctgtgaag
     6361 tttcttctcc ttccatggaa atggggaatg aagtatttct gggatacact tttggatgct
     6421 gatttggaat gtgacatcct tggctggcag tatatctctg ggagtatccc cgatggccac
     6481 gagcttgatc gcttggacaa tcccgcgtta caaggcgcca aatatgaccc agaaggtgag
     6541 tacataaggc aatggcttcc cgagcttgcg agattgccaa ctgaatggat ccatcatcca
     6601 tgggacgctc ctttaaccgt actcaaagct tctggtgtgg aactcggaac aaactatgcg
     6661 aaacccattg tagacatcga cacagctcgt gagctactag ctaaagctat ttcaagaacc
     6721 cgtgaagcac agatcatgat cggagcagca gcccggggat cctaactcga ggccaattgg
     6781 tgcggcaatt gataataacg aaaatgtctt ttaatgatct gggtataatg aggaattttc
     6841 cgaacgtttt tactttatat atatatatac atgtaacata tattctatac gctatagaga
     6901 aaggaaattt ttcaattaaa aaaaaaatag agaaagagtt tcacttcttg attatcgcta
     6961 acactaatgg ttgaagtact gctactttaa ttttatgctg gatgcacaca ctggcttaag
     7021 atgacaacga agaggatgtc caatattttt tttaaggaat aaggatactt caagactaga
     7081 ttcccccctg cattcccatc agaaccgtaa accttggcgc tttccttggg aagtattcaa
     7141 gaagtgcctt gtccggtttc tgtggctcac aaaccagcgc gcccgatatg gctttctttt
     7201 cacttatgaa tgtaccagta cgggacaatt agaacgctcc tgtaacaatc tctttgcaaa
     7261 tgtggggtta cattctaacc atgtcacact gctgacgaaa ttcaaagtaa aaaaaaatgg
     7321 gaccacgtct tgagaacgat agattttctt tattttacat tgaacagtcg ttgtctcagc
     7381 gcgctttatg ttttcattca tacttcatat tataaaataa caaaagaaga atttcatatt
     7441 cacgcccaag aaatcaggct gctttccaaa tgcaattgac acttcattag ccatcacaca
     7501 aaactctttc ttgctggagc ttcttttaaa aaagacctca gtacaccaaa cacgttaccc
     7561 gacctcgtta ttttacgaca actatgataa aattctgaag aaaaaataaa aaaattttca
     7621 tacttcttgc ttttatttaa accattgaat gatttctttt gaacaaaact acctgtttca
     7681 ccaaaggaaa tagaaagaaa aaatcaatta gaagaaaaca aaaaacaaaa gatctatggt
     7741 gtccaaagga gaggagttaa tcaaggaaaa catgagaatg aaagttgtca tggagggctc
     7801 cgttaatggt caccaattca agtgtacagg ggaaggtgaa ggtaatcctt acatgggtac
     7861 acaaactatg agaattaaag taattgaagg cggaccacta ccatttgcat ttgacattct
     7921 ggcaacgtca ttcatgtacg gatcacgaac tttcatcaag taccctaaag gtataccaga
     7981 ctttttcaag caatcttttc cagagggttt tacatgggaa agggttacaa gatacgaaga
     8041 tgggggtgtc gtcacagtta tgcaagatac ttcattagaa gatggctgcc ttgtctatca
     8101 tgtgcaagta agaggggtga attttccttc taacggacct gtgatgcaga aaaagaccaa
     8161 aggttgggaa ccaaatactg aaatgatgta cccagctgat ggaggtttga gaggctacac
     8221 acacatggcg cttaaagttg atggtggagg tcatttgtct tgtagttttg ttaccactta
     8281 tcgttctaaa aagactgttg gcaatatcaa aatgccagga atacatgctg tagaccacag
     8341 actagaaaga ctcgaagaga gcgataacga aatgttcgtt gtacagagag agcatgccgt
     8401 agccaaattt gctggcttag gcggtggtat ggatgaattg tataagggat cctaactcga
     8461 ggcgaatttc ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt
     8521 atacaaattt taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc
     8581 tttcctgtag gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac
     8641 ctctaccggc atgccgagca aatgcctgca aatcgctccc catttcgctg agcagttaca
     8701 gagatgttac gaaccactag tgcactgcag tacacggttt ccttgaaatt tttttgattc
     8761 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     8821 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     8881 aacaaaaacg tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
     8941 tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac
     9001 aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc
     9061 attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat
     9121 ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga
     9181 ggacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata
     9241 cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt
     9301 tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt
     9361 agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga
     9421 cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg
     9481 aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg
     9541 agatgcattg ggtcaacagt atagaaccgt ggatgatgtg gtttctacag gatctgacat
     9601 tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg
     9661 ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac
     9721 tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
     9781 tcagttatta cccgagtaga gcacttgaat ccactgcccc gggaatctcg gtcgtaatga
     9841 tttctataat gacgaaaaaa aaaaaattgg aaagaaaaag cttcatggcc tttataaaaa
     9901 ggaactatcc aatacctcgc cagaaccaag taacagtatt ttacggggca caaatcaaga
     9961 acaataagac aggactgtaa agatggacgc attgaactcc aaagaacaac aagagttcca
    10021 aaaagtagtg gaacaaaagc aaatgaagga tttcatgcgt ttgtactcta atctggtaga
    10081 aagatgtttc acagactgtg tcaatgactt cacaacatca aagctaacca ataaggaaca
    10141 aacatgcatc atgaagtgct cagaaaagtt cttgaagcat agcgaacgtg tagggcagcg
    10201 tttccaagaa caaaacgctg ccttgggaca aggcttgggc cgataaggtg tactggcgta
    10261 tatatatcta attatgtatc tctggtgtag cccattttta gcatgtaaat ataaagaccg
    10321 agcggccgcg tgttacaacc aattaaccaa ttctgattag aaaaactcat cgagcatcaa
    10381 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
    10441 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
    10501 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
    10561 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
    10621 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
    10681 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg
    10741 atcgctgtta aaaggacaat tacaaac
//
