LOCUS       sequence-247362-       10747 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control for optogenetic machinery 
            (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration with pSTRONG-mRUBY2
            reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10747)
  AUTHORS   An-Adirekkun JM, Stewart CJ, Geller SH, Patel MT, Melendez J, Oakes 
            BL, Noyes MB, McClean MN
  TITLE     A yeast optogenetic toolkit (yOTK) for gene expression control in 
            Saccharomyces cerevisiae.
  JOURNAL   Biotechnol Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234.
  PUBMED    31788779
REFERENCE   2  (bases 1 to 10747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Bioeng. 2019 Dec 2. doi: 10.1002/bit.27234."
COMMENT     Sequence Label: pMM0655
FEATURES             Location/Qualifiers
     source          1..10747
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     313..332
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     397..416
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      630..1218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1119..1138
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             2634..2654
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     2882..2901
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2882..2900
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2925..3434
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKKRKFDTETKDCN
                     EKKKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
     CDS             4954..4974
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5257..6750
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     promoter        7043..7709
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     7579..7602
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             7716..8426
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     terminator      8442..8629
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(8497..8521)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             8892..9695
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(join(10337..10747,1..405))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(10337..10747,1..405))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga
       61 atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa
      121 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt
      181 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg
      241 tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga
      301 ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt
      361 taatcgcggc ctggagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt
      421 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat
      481 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga
      541 gttgaaggat cagtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
      781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtgcg
     1321 gccgccaatg ctaaattcga gtgaaacaca ggaagatcag aaaatcctca tttcatccat
     1381 attaacaata atttcaaatg tttatttgca ttatttgaaa ctaggcaaga caagcaacga
     1441 aacgtttttg aaaattttga gtattttcaa taaatttgta gaggactcag atattgaaaa
     1501 aaagctacag caattaatac ttgataagaa gagtattgag aagggcaacg gttcatcatc
     1561 tcatggatct gcacatgaac aaacaccaga gtcaaacgac gttgaaattg aggctactgc
     1621 gccaattgat gacaatacag acgatgataa caaaccgaag ttatctgatg tagaaaagga
     1681 ttaaagatgc taagagatag tgatgatatt tcataaataa tgtaattcta tatatgttaa
     1741 ttaccttttt tgcgaggcat atttatggtg aaggataagt tttgaccatc aaagaaggtt
     1801 aatgtggctg tggtttcagg gtccataaag cccacatgga taacattacc cctgaattcg
     1861 catctagact gaactggccg ataattgcag acgaacgaag aggatgtcca atattttttt
     1921 taaggaataa ggatacttca agactagatt cccccctgca ttcccatcag aaccgtaaac
     1981 cttggcgctt tccttgggaa gtattcaaga agtgccttgt ccggtttctg tggctcacaa
     2041 accagcgcgc ccgatatggc tttcttttca cttatgaatg taccagtacg ggacaattag
     2101 aacgctcctg taacaatctc tttgcaaatg tggggttaca ttctaaccat gtcacactgc
     2161 tgacgaaatt caaagtaaaa aaaaatggga ccacgtcttg agaacgatag attttcttta
     2221 ttttacattg aacagtcgtt gtctcagcgc gctttatgtt ttcattcata cttcatatta
     2281 taaaataaca aaagaagaat ttcatattca cgcccaagaa atcaggctgc tttccaaatg
     2341 caattgacac ttcattagcc atcacacaaa actctttctt gctggagctt cttttaaaaa
     2401 agacctcagt acaccaaaca cgttacccga cctcgttatt ttacgacaac tatgataaaa
     2461 ttctgaagaa aaaataaaaa aattttcata cttcttgctt ttatttaaac cattgaatga
     2521 tttcttttga acaaaactac ctgtttcacc aaaggaaata gaaagaaaaa atcaattaga
     2581 agaaaacaaa aaacaaaaga tctatggata aagcggaatt aattcccgag cctccaaaaa
     2641 agaagagaaa ggtcgaattg ggtaccgccg cctcggagct ccacttagac ggcgaggacg
     2701 tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg
     2761 attccccggg tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg
     2821 ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggt
     2881 ttaatacgac tcactatagg gcgagcgccg aagctagcgc caccatgaat ggagctatag
     2941 gaggtgacct tttgctcaat tttcctgaca tgtcggtcct agagcgccaa agggctcacc
     3001 tcaagtacct caatcccacc tttgattctc ctctcgccgg cttctttgcc gattcttcaa
     3061 tgattaccgg cggcgagatg gacagctatc tttcgactgc cggtttgaat cttccgatga
     3121 tgtacggtga aacgacggtg gaaggtgatt caagactctc aatttcgccg gaaacgacgc
     3181 ttgggactgg aaatttcaag aaacggaagt ttgatacaga gactaaggat tgtaatgaga
     3241 agaagaagaa gatgacgatg aacagagatg acctagtaga agaaggagaa gaagagaagt
     3301 cgaaaataac agagcaaaac aatgggagca caaaaagcat caagaagatg aaacacaaag
     3361 ccaagaaaga agagaacaat ttctctaatg attcatctaa agtgacgaag gaattggaga
     3421 aaacggatta tattcatgtt cgtgcacgac gaggccaagc cactgatagt cacagcatag
     3481 cagaacgagt tagaagagaa aagatcagtg agagaatgaa gtttctacaa gatttggttc
     3541 ctggatgcga caagatcaca ggcaaagcag ggatgcttga tgaaatcatt aactatgttc
     3601 agtctcttca gagacaaatc gagttcttat cgatgaaact agcaattgtg aatccaaggc
     3661 cggattttga tatggatgac atttttgcca aagaggttgc ctcaactcca atgactgtgg
     3721 tgccatctcc tgaaatggtt ctttccggtt attctcatga gatggttcac tctggttatt
     3781 ctagtgagat ggttaactcc ggttaccttc atgtcaatcc aatgcagcaa gtgaatacca
     3841 gttctgatcc attgtcatgc ttcaacaatg gcgaagctcc ttcgatgtgg gactctcatg
     3901 tgcagaatct ctatggcaat ttaggagtac cggtcatcga gctcgagctg cagatgaatc
     3961 gtagatacgg atcctaactc gagagctttt gattaagcct tctagtccaa aaaacacgtt
     4021 tttttgtcat ttatttcatt ttcttagaat agtttagttt attcatttta tagtcacgaa
     4081 tgttttatga ttctatatag ggttgcaaac aagcattttt cattttatgt taaaacaatt
     4141 tcaggtttac cttttattct gcttgtggtg acgcgtgtat ccgcccgctc ttttggtcac
     4201 ccatgtatgc tgccaaacca gatgtcaaca cagctacaac gaagaggatg tccaatattt
     4261 tttttaagga ataaggatac ttcaagacta gattcccccc tgcattccca tcagaaccgt
     4321 aaaccttggc gctttccttg ggaagtattc aagaagtgcc ttgtccggtt tctgtggctc
     4381 acaaaccagc gcgcccgata tggctttctt ttcacttatg aatgtaccag tacgggacaa
     4441 ttagaacgct cctgtaacaa tctctttgca aatgtggggt tacattctaa ccatgtcaca
     4501 ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt cttgagaacg atagattttc
     4561 tttattttac attgaacagt cgttgtctca gcgcgcttta tgttttcatt catacttcat
     4621 attataaaat aacaaaagaa gaatttcata ttcacgccca agaaatcagg ctgctttcca
     4681 aatgcaattg acacttcatt agccatcaca caaaactctt tcttgctgga gcttctttta
     4741 aaaaagacct cagtacacca aacacgttac ccgacctcgt tattttacga caactatgat
     4801 aaaattctga agaaaaaata aaaaaatttt catacttctt gcttttattt aaaccattga
     4861 atgatttctt ttgaacaaaa ctacctgttt caccaaagga aatagaaaga aaaaatcaat
     4921 tagaagaaaa caaaaaacaa aagatctatg gcccccaaga agaagaggaa ggttggtatt
     4981 cacgggggta cccgcccata tgcttgccct gtcgagtcct gcgatcgccg cttttctcgc
     5041 tcggatgagc ttacccgcca tatccgcatc cataccggtc agaagccctt ccagtgtcga
     5101 atctgcatgc gtaacttcag tcgtagtgac caccttacca cccacatccg cacccacaca
     5161 ggcgagaagc cttttgcctg tgacatttgt gggaggaagt ttgccaggag tgatgaacgc
     5221 aagaggcata ccaaaatcca tacaggtgct agcttcatga agatggacaa aaagactata
     5281 gtttggttta gaagagatct aaggattgag gataatcctg cattagcagc agctgctcac
     5341 gaaggatctg tttttcctgt cttcatttgg tgtcctgaag aagaaggaca gttttatcct
     5401 ggaagagctt caagatggtg gatgaaacaa tcacttgctc acttatctca atccttgaag
     5461 gctcttggat ctgacctcac tttaatcaaa acccacaaca cgatttcagc gatcttggat
     5521 tgtatccgcg ttaccggtgc tacaaaagtc gtctttaacc acctctatga tcctgtttcg
     5581 ttagttcggg accataccgt aaaggagaag ctggtggaac gtgggatctc tgtgcaaagc
     5641 tacaatggag atctattgta tgaaccgtgg gagatatact gcgaaaaggg caaacctttt
     5701 acgagtttca attcttactg gaagaaatgc ttagatatgt cgattgaatc cgttatgctt
     5761 cctcctcctt ggcggttgat gccaataact gcagcggctg aagcgatttg ggcgtgttcg
     5821 attgaagaac tagggctgga gaatgaggcc gagaaaccga gcaatgcgtt gttaactaga
     5881 gcttggagtc caggatggag caatgctgat aagttactaa atgagttcat cgagaagcag
     5941 ttgatagatt atgcaaagaa cagcaagaaa gttgttggga attctacttc actactttct
     6001 ccgtatctcc atttcgggga aataagcgtc agacacgttt tccagtgtgc ccggatgaaa
     6061 caaattatat gggcaagaga taagaacagt gaaggagaag aaagtgcaga tctttttctt
     6121 aggggaatcg gtttaagaga gtattctcgg tatatatgtt tcaacttccc gtttactcac
     6181 gagcaatcgt tgttgagtca tcttcggttt ttcccttggg atgctgatgt tgataagttc
     6241 aaggcctgga gacaaggcag gaccggttat ccgttggtgg atgccggaat gagagagctt
     6301 tgggctaccg gatggatgca taacagaata agagtgattg tttcaagctt tgctgtgaag
     6361 tttcttctcc ttccatggaa atggggaatg aagtatttct gggatacact tttggatgct
     6421 gatttggaat gtgacatcct tggctggcag tatatctctg ggagtatccc cgatggccac
     6481 gagcttgatc gcttggacaa tcccgcgtta caaggcgcca aatatgaccc agaaggtgag
     6541 tacataaggc aatggcttcc cgagcttgcg agattgccaa ctgaatggat ccatcatcca
     6601 tgggacgctc ctttaaccgt actcaaagct tctggtgtgg aactcggaac aaactatgcg
     6661 aaacccattg tagacatcga cacagctcgt gagctactag ctaaagctat ttcaagaacc
     6721 cgtgaagcac agatcatgat cggagcagca gcccggggat cctaactcga ggccaattgg
     6781 tgcggcaatt gataataacg aaaatgtctt ttaatgatct gggtataatg aggaattttc
     6841 cgaacgtttt tactttatat atatatatac atgtaacata tattctatac gctatagaga
     6901 aaggaaattt ttcaattaaa aaaaaaatag agaaagagtt tcacttcttg attatcgcta
     6961 acactaatgg ttgaagtact gctactttaa ttttatgctg gatgcacaca ctggcttaag
     7021 atgacaacgc agttcgagtt tatcattatc aatactgcca tttcaaagaa tacgtaaata
     7081 attaatagta gtgattttcc taactttatt tagtcaaaaa attagccttt taattctgct
     7141 gtaacccgta catgcccaaa atagggggcg ggttacacag aatatataac atcgtaggtg
     7201 tctgggtgaa cagtttattc ctggcatcca ctaaatataa tggagcccgc tttttaagct
     7261 ggcatccaga aaaaaaaaga atcccagcac caaaatattg ttttcttcac caaccatcag
     7321 ttcataggtc cattctctta gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac
     7381 gggcacaacc tcaatggagt gatgcaacct gcctggagta aatgatgaca caaggcaatt
     7441 gacccacgca tgtatctatc tcattttctt acaccttcta ttaccttctg ctctctctga
     7501 tttggaaaaa gctgaaaaaa aaggttgaaa ccagttccct gaaattattc ccctacttga
     7561 ctaataagta tataaagacg gtaggtattg attgtaattc tgtaaatcta tttcttaaac
     7621 ttcttaaatt ctacttttat agttagtctt ttttttagtt ttaaaacacc aagaacttag
     7681 tttcgaataa acacacataa acaaacaaaa gatctatggt gtccaaagga gaggagttaa
     7741 tcaaggaaaa catgagaatg aaagttgtca tggagggctc cgttaatggt caccaattca
     7801 agtgtacagg ggaaggtgaa ggtaatcctt acatgggtac acaaactatg agaattaaag
     7861 taattgaagg cggaccacta ccatttgcat ttgacattct ggcaacgtca ttcatgtacg
     7921 gatcacgaac tttcatcaag taccctaaag gtataccaga ctttttcaag caatcttttc
     7981 cagagggttt tacatgggaa agggttacaa gatacgaaga tgggggtgtc gtcacagtta
     8041 tgcaagatac ttcattagaa gatggctgcc ttgtctatca tgtgcaagta agaggggtga
     8101 attttccttc taacggacct gtgatgcaga aaaagaccaa aggttgggaa ccaaatactg
     8161 aaatgatgta cccagctgat ggaggtttga gaggctacac acacatggcg cttaaagttg
     8221 atggtggagg tcatttgtct tgtagttttg ttaccactta tcgttctaaa aagactgttg
     8281 gcaatatcaa aatgccagga atacatgctg tagaccacag actagaaaga ctcgaagaga
     8341 gcgataacga aatgttcgtt gtacagagag agcatgccgt agccaaattt gctggcttag
     8401 gcggtggtat ggatgaattg tataagggat cctaactcga ggcgaatttc ttatgattta
     8461 tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact
     8521 cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc
     8581 tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggc atgccgagca
     8641 aatgcctgca aatcgctccc catttcgctg agcagttaca gagatgttac gaaccactag
     8701 tgcactgcag tacacggttt ccttgaaatt tttttgattc ggtaatctcc gaacagaagg
     8761 aagaacgaag gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag
     8821 aaacatgaaa ttgcccagta ttcttaaccc aactgcacag aacaaaaacg tgcaggaaac
     8881 gaagataaat catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg
     8941 ttgctgccaa gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg
     9001 atgttcgtac caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt
     9061 tactaaaaac acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc
     9121 taaaggcatt atccgccaag tacaattttt tactcttcga ggacagaaaa tttgctgaca
     9181 ttggtaatac agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag
     9241 acattacgaa tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg
     9301 cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg
     9361 gctccctatc tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag
     9421 attttgttat cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt
     9481 ggttgattat gacacccggt gtgggtttag atgacaaggg agatgcattg ggtcaacagt
     9541 atagaaccgt ggatgatgtg gtttctacag gatctgacat tattattgtt ggaagaggac
     9601 tatttgcaaa gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg
     9661 aagcatattt gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat
     9721 gtatactaaa ctcacaaatt agagcttcaa tttaattata tcagttatta cccgagtaga
     9781 gcacttgaat ccactgcccc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa
     9841 aaaaaattgg aaagaaaaag cttcatggcc tttataaaaa ggaactatcc aatacctcgc
     9901 cagaaccaag taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa
     9961 agatggacgc attgaactcc aaagaacaac aagagttcca aaaagtagtg gaacaaaagc
    10021 aaatgaagga tttcatgcgt ttgtactcta atctggtaga aagatgtttc acagactgtg
    10081 tcaatgactt cacaacatca aagctaacca ataaggaaca aacatgcatc atgaagtgct
    10141 cagaaaagtt cttgaagcat agcgaacgtg tagggcagcg tttccaagaa caaaacgctg
    10201 ccttgggaca aggcttgggc cgataaggtg tactggcgta tatatatcta attatgtatc
    10261 tctggtgtag cccattttta gcatgtaaat ataaagaccg agcggccgcg tgttacaacc
    10321 aattaaccaa ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca
    10381 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
    10441 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
    10501 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
    10561 caccatgagt gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga
    10621 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
    10681 tattcattcg tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat
    10741 tacaaac
//
