LOCUS       sequence-247412-        3741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human JARID2 in insect cells, , under a C3-cleavable 
            (PreScission Protease) N-terminal hexahistidine-MBP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, 
            Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, 
            Davidovich C
  TITLE     RNA exploits an exposed regulatory site to inhibit the enzymatic 
            activity of PRC2.
  JOURNAL   Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 
            10.1038/s41594-019-0197-y. Epub 2019 Mar 4.
  PUBMED    30833789
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0197-y"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-03"; volume: "26"; issue: "3"; pages: "237-247"
COMMENT     Sequence Label: pFB1.HMBP.PrS.JARID2
FEATURES             Location/Qualifiers
     source          1..3741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1257..1265
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 atgagcaagg aaagacccaa gaggaatatc attcagaaga aatacgatga cagtgatggg
       61 attccgtggt cagaagaacg ggtggtacgt aaagtccttt atttgtctct gaaggagttc
      121 aagaattccc agaagaggca gcatgcggaa ggcattgctg ggagcctgaa aactgtgaat
      181 gggctccttg gtaatgacca gtctaaggga ttaggaccag catcagaaca gtcagagaat
      241 gaaaaggacg atgcatccca agtgtcctcc actagcaacg atgttagttc ttcagatttt
      301 gaagaagggc cgtcgaggaa aaggcccagg ctgcaagcac aaaggaagtt tgctcagtct
      361 cagccgaata gtcccagcac aactccagta aagatagtgg agccattgct accccctcca
      421 gctactcaga tatcagacct ctctaaaagg aagcctaaga cagaagattt tcttaccttt
      481 ctctgccttc gaggttctcc tgcgctgccc aacagcatgg tgtattttgg aagctctcag
      541 gatgaggagg aagtcgagga ggaagatgat gagacagaag acgtcaaaac agccaccaac
      601 aatgcttcat cttcatgcca gtcgaccccc aggaaaggaa aaacccacaa acatgttcac
      661 aacgggcatg ttttcaatgg ttccagcagg tcaacacggg agaaggaacc tgttcaaaaa
      721 cacaaaagca aagaggccac tcccgcaaag gagaagcaca gcgatcaccg ggctgacagc
      781 cgccgggagc aggcttcagc taaccacccc gcagcggccc cctccacggg ttcctcggcc
      841 aaggggcttg ctgccaccca tcaccacccc cctctgcatc ggtcggctca ggacttacgg
      901 aaacaggttt ctaaggtaaa cggagtcact cgaatgtcat ctctgggtgc aggtgtaacc
      961 agtgccaaaa agatgcgcga ggtcagacct tcaccatcca aaactgtgaa gtacactgcc
     1021 acggtgacga agggggctgt cacatacacc aaagccaaga gagaactggt caaggacacc
     1081 aaacccaatc accacaagcc cagttccgct gtcaaccaca caatctcagg gaaaactgaa
     1141 agtagcaatg caaaaacccg caaacaggtg ctatccctcg ggggggcgtc caagtccact
     1201 gggcccgccg tcaatggcct caaggtcagt ggcaggttga acccaaagtc atgcactaag
     1261 gaggtggggg ggcggcagct gcgggagggc ctgcagctgc gggaggggct gcggaactcc
     1321 aagaggagac tggaagaggc acaccaggcg gagaagccgc agtcgccccc caagaagatg
     1381 aaaggggcgg ctggccccgc cgaaggccct ggcaagaagg ccccggccga gagaggtctg
     1441 ctgaacggac acgtgaagaa ggaagtgccg gagcgcagtc tggagaggaa tcggccgaag
     1501 cgggccacgg ccgggaagag cacgccaggc agacaagcac atggcaaggc ggacagcgcc
     1561 tcctgtgaaa atcgttctac ctcgcaaccg gagtccgtgc acaagccgca ggactcgggc
     1621 aaggccgaga agggcggcgg caaggccggg tgggcggcca tggacgagat ccccgtcctc
     1681 aggccctccg ccaaggagtt ccacgatccg ctcatctaca tcgagtcggt ccgcgctcag
     1741 gtggagaagt tcgggatgtg cagggtgatc ccccctccgg actggcggcc cgagtgcaag
     1801 ctcaacgatg agatgcggtt tgtcacgcag attcagcaca tccacaagct gggccggcgc
     1861 tggggcccca acgtgcagcg gctggcctgc atcaagaagc acctcaaatc tcagggcatc
     1921 accatggacg agctcccgct catagggggc tgtgagctcg acctggcctg ctttttccgg
     1981 ctgattaatg agatgggcgg catgcagcaa gtgactgacc tcaaaaaatg gaacaaacta
     2041 gcagacatgc tgcgcatccc cagaactgcc caggaccggc tggccaagct gcaggaggcc
     2101 tactgccagt acctactctc ctacgactcc ctgtccccag aggagcaccg gcggctggag
     2161 aaggaggtgc tgatggagaa ggagatcctg gagaagcgca aggggccgct ggaaggccac
     2221 acagagaacg accaccacaa gttccaccct ctgccccgct tcgagcccaa gaatgggctc
     2281 atccacggcg tggcccccag gaacggcttc cgcagcaagc tcaaggaggt gggccaggcc
     2341 cagttgaaga ctggccggcg gcgactcttc gctcaggaaa aagaagtggt caaggaagag
     2401 gaggaggaca aaggcgtcct caatgacttc cacaagtgca tctataaggg aaggtctgtt
     2461 tctctaacaa ctttttatcg aacagcgagg aatatcatga gcatgtgttt cagcaaggag
     2521 cctgccccag ccgaaatcga gcaagagtac tggaggctag tggaagagaa ggactgccac
     2581 gtggcagtgc actgcggcaa ggtggacacc aacactcacg gcagtggatt cccagtagga
     2641 aaatcagaac ccttttcgag gcatggatgg aacctcaccg tcctccccaa taacacaggg
     2701 tccatcctgc gtcacctcgg tgctgtgcct ggagtgacta ttccctggct aaatattggc
     2761 atggtctttt ctacctcatg ctggtctcga gaccaaaatc accttccata cattgactac
     2821 ttacacactg gtgctgactg catttggtat tgcattcctg ctgaggagga gaacaagctg
     2881 gaagatgtgg tccacaccct gctgcaagcc aatggcaccc cagggctgca gatgctggaa
     2941 agcaacgtca tgatctcccc ggaggtgctg tgcaaagagg ggatcaaggt gcacaggacc
     3001 gtgcagcaga gtggccagtt tgtcgtctgc ttcccgggat cctttgtgtc caaagtgtgc
     3061 tgtgggtaca gcgtgtctga aaccgtgcac tttgctacca cccagtggac aagtatgggc
     3121 tttgagaccg ccaaggaaat gaagcgtcgc catatagcta agccattctc catggagaag
     3181 ttactctacc agattgcaca agcagaagca aaaaaagaaa acggtcccac tctcagtacc
     3241 atctcagccc tcctggatga gctcagggat acagagctgc ggcagcgcag gcagctgttc
     3301 gaggctggcc tccactcctc cgcacgctat ggcagccacg atggcagcag cacggtggcg
     3361 gacgggaaga aaaagcctcg aaagtggctg cagttggaga cgtcagagag gaggtgtcag
     3421 atctgccagc acctgtgcta cctgtccatg gtggtacaag agaacgaaaa cgtcgtgttc
     3481 tgtctggagt gtgctctgcg ccacgtggag aaacagaagt cctgccgagg gctgaagttg
     3541 atgtaccgct acgatgagga acagattatc agtctggtca atcagatctg cggcaaagtg
     3601 tctggtaaaa acggcagcat tgagaactgt ctcagtaaac ccacaccaaa aagaggtccc
     3661 cgcaagagag cgacagtgga cgtgcccccc tcccgtctgt cagcctccag ttcatccaaa
     3721 agtgcttcga gctcatcatg a
//
