LOCUS       sequence-247414-        1731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human PHF1 in insect cells, , under a C3-cleavable 
            (PreScission Protease) N-terminal hexahistidine-MBP tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Zhang Q, McKenzie NJ, Warneford-Thomson R, Gail EH, Flanigan SF, 
            Owen BM, Lauman R, Levina V, Garcia BA, Schittenhelm RB, Bonasio R, 
            Davidovich C
  TITLE     RNA exploits an exposed regulatory site to inhibit the enzymatic 
            activity of PRC2.
  JOURNAL   Nat Struct Mol Biol. 2019 Mar;26(3):237-247. doi: 
            10.1038/s41594-019-0197-y. Epub 2019 Mar 4.
  PUBMED    30833789
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41594-019-0197-y"; journalName: "Nat Struct Mol Biol"; 
            date: "2019-03"; volume: "26"; issue: "3"; pages: "237-247"
COMMENT     Sequence Label: pFB1.HMBP.PrS.PHF1
FEATURES             Location/Qualifiers
     source          1..1731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    457..471
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
ORIGIN
        1 ggtctccccg gggcgcagcc cccccggctg agccgctctg gtgcctcctc actttgggac
       61 ccagcttctc ctgctcccac ctctggcccc aggcctcggc tttgggaggg tcaagatgtg
      121 ctggccagat ggactgatgg gctgctatac ttgggtacca tcaaaaaggt ggacagtgct
      181 agggaggtgt gtctggtcca gtttgaggat gattcgcagt ttctggttct atggaaagac
      241 attagccctg ctgccctccc tggagaggaa ctcctctgtt gtgtctgtcg ctctgagact
      301 gtggtccctg ggaaccggct ggtcagctgt gagaagtgtc gccatgctta tcaccaggac
      361 tgccatgttc ccagggctcc agcccctgga gagggagagg gcacatcctg ggtatgccgc
      421 cagtgtgtct ttgcgatcgc caccaagagg ggaggtgccc tgaagaaggg cccctatgcc
      481 cgggccatgc tgggtatgaa gctttctctg ccatatggac tgaaggggct ggactgggat
      541 gctggacatc tgagcaaccg acagcagagt tactgttact gtggtggccc tggggagtgg
      601 aacctgaaaa tgctgcagtg ccggagctgc ctgcagtggt tccatgaggc ctgcacccag
      661 tgtctgagca agcccctcct ctatggggac aggttctatg aatttgaatg ctgtgtgtgt
      721 cgcgggggcc ctgagaaagt ccggagacta cagcttcgct gggtggatgt ggcccatctt
      781 gtcctgtatc acctcagtgt ttgctgtaag aagaaatact ttgattttga tcgtgagatc
      841 ctccccttca cttctgagaa ttgggacagt ttgctcctgg gggagctttc agacaccccc
      901 aaaggagaac gttcttccag gctcctctct gctcttaaca gccacaagga ccgtttcatt
      961 tcagggagag agattaagaa gaggaaatgt ttgtttggtc tccatgctcg gatgcctccc
     1021 cctgtggagc cccctactgg agatggagca ctcaccagct tcccttcagg gcagggccct
     1081 gggggagggg tctcacgtcc cctggggaag cgccggaggc cggagccaga gcccctgagg
     1141 aggaggcaga aggggaaagt ggaggagctg gggccaccct cagcagtgcg caatcagccc
     1201 gagccccagg agcagaggga gcgggctcat ctgcagaggg cactgcaggc ctcagtgtct
     1261 ccaccatccc ccagccctaa ccagagttac cagggcagca gcggctacaa cttccggccc
     1321 acagatgccc gctgcctgcc cagcagcccc atccggatgt ttgcttcctt ccacccttct
     1381 gccagcaccg cagggacctc tggggacagt ggacccccag acaggtcacc cctggaactt
     1441 cacattggtt tccccacaga catccctaaa agtgcccccc actcgatgac tgcctcatct
     1501 tcctcagttt catccccatc cccaggtctt cctagacgct cagcaccccc ttctcccctg
     1561 tgccgtagtt tgtctcctgg gactggggga ggagtccgag gtggggttgg ttacctgtcc
     1621 cgaggggacc ctgtccgggt ccttgctcgg agagtacggc ctgatggctc tgtgcagtac
     1681 ctggttgagt ggggaggagg gggcatcttc tgataatagc tcgaggagac c
//
