LOCUS       sequence-247431-        6911 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse CD72a in animal cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6911)
  TITLE     Tsubata CD72, SmB, and U1-C plasmids
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMXs-CD72a-IRES-hCD8
FEATURES             Location/Qualifiers
     source          1..6911
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             22..615
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    678..1035
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1094..1510
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1440..1462
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1478..1494
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1520..1894
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1832..1848
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(3048..3622,3623..3625,3626..3634)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3215..3232)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3442..3461
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     LTR             4417..4886
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5016..5033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5187..5775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5267..5286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5939..6730,6731..6799))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6562..6581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6800..6904)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta
       61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc
      121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      181 ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat
      241 atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg
      301 gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct
      361 gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg
      421 cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc gcgccagtcc
      481 tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc
      541 cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag
      601 cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca gggaccaccg
      661 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg
      721 tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct ctgtatctgg
      781 cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg gagacgtccc
      841 agggacttcg ggggccgttt ttgtggcccg acctgagtcc aaaaatcccg atcgttttgg
      901 actctttggt gcacccccct aataggaggg atatgtggtt ctggtaggag acgagaacct
      961 aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc
     1021 gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt
     1081 gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     1141 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     1201 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     1261 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     1321 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     1381 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     1441 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     1501 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     1561 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     1621 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     1681 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     1741 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     1801 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     1861 tgccgacccc gggggtggac catcctctag actgccggat ctagctagtt aattaaggat
     1921 cccagtgtgg tggtacggga attcatggct gacgctatca cgtatgcaga cctgcgcttt
     1981 gtgaaagtgc ccctgaagaa cagcgcatct aaccatctag gacaggactg tgaggcctat
     2041 gaagatgggg aactcaccta cgagaacgtg caagtgtctc cagtcccagg agggccacca
     2101 ggcttggctt cccctgcact agcggacaaa gcaggggtcg ggtcagagca accaactgcg
     2161 acctggagct ctgtgaagtc gtctgctctc aggcagattc cccgctgtcc tacggtctgc
     2221 ttgcaaaact tcttgcttgg ccttctcctg tcctgtctga tgttaggggt ggctgtcatc
     2281 tgcctgggag ttcgctatct gcaggtgtct cagcagttcc aggaggggac caggatttgg
     2341 gaagccacca atagcagcct gcagcagcag ctcagggaga agataagtca gctggggcag
     2401 aaggaggtgg agcttcagga gtctcagaaa gagctgatct cgagccagga cacattacag
     2461 gagaagcaga ggactcacaa ggacactgag cagcaactac aagcctgcca ggctgagaga
     2521 gcgaagacca aggagaacct gaaaactgag gaggagcgga ggagggacct ggaccagagg
     2581 ttgacaagca cgcgggagac actgaggcgc ttgtcctcct gttcatcaga cacctgctgt
     2641 ccatgcggat ggattccata tcaggaaagg tgcttttaca tctcacatac cctcagaagt
     2701 ctggaggaga gccaaaaata ctgcacatct ctgtcctcca aactggcagc attcgatgaa
     2761 ccttctaagt attactatga agtttctctg cccagcggct tagaggagtt gctagatcgt
     2821 tcgaagtcat attggataca gatgagcaag aagtggaggc atgactatga ctctcaaagc
     2881 cgatattgtg acaagataaa aaaatattac cagaagtgga aaagaacatt ttctgagtgt
     2941 gcagagcttc acccctgcat ttgtgagtcg gaggctttca ggtttcctga tgggatccat
     3001 ctgaactgaa ccggatagcg gccgctacgt aaattccgcc cccccccccc ctctccctcc
     3061 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     3121 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     3181 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     3241 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     3301 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     3361 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     3421 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     3481 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     3541 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc
     3601 ctttgaaaaa cacgatgata atatggccac aaccatggcc ttaccagtga ccgccttgct
     3661 cctgccgctg gccttgctgc tccacgccgc caggccgagc cagttccggg tgtcgccgct
     3721 ggatcggacc tggaacctgg gcgagacagt ggagctgaag tgccaggtgc tgctgtccaa
     3781 cccgacgtcg ggctgctcgt ggctcttcca gccgcgcggc gccgccgcca gtcccacctt
     3841 cctcctatac ctctcccaaa acaagcccaa ggcggccgag gggctggaca cccagcggtt
     3901 ctcgggcaag aggttggggg acaccttcgt cctcaccctg agcgacttcc gccgagagaa
     3961 cgagggctac tatttctgct cggccctgag caactccatc atgtacttca gccacttcgt
     4021 gccggtcttc ctgccagcga agcccaccac gacgccagcg ccgcgaccac caacaccggc
     4081 gcccaccatc gcgtcgcagc ccctgtccct gcgcccagag gcgtgccggc cagcggcggg
     4141 gggcgcagtg cacacgaggg ggctggactt cgcctgtgat atctacatct gggcgccctt
     4201 ggccgggact tgtggggtcc ttctcctgtc actggttatc acctaactcg acgataaaat
     4261 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     4321 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     4381 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     4441 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     4501 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     4561 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     4621 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     4681 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     4741 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     4801 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     4861 gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt
     4921 ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg
     4981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     5881 cttttgcggc cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     5941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6001 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     6061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     6121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     6181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     6241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     6301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     6361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     6421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     6481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     6541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     6601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     6661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     6721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     6781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     6841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     6901 cgcgcacatt t
//
