LOCUS       sequence-247477-       16285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16285)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX135
FEATURES             Location/Qualifiers
     source          1..16285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6399..6423)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6452..6541
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6575..6827
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7256..7508)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7976..9001)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7976..9001)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9011..9355)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9497..9514)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9497..9513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9506..9528)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9653..9672)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9863..9884
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10131..10155
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11751..12899)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13198..13992)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14184..14951
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15644..16275
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatggc agattcggac aatgaatcag gagggcagag
     5881 agagaatgag agttcactaa gagagcaaga caggttcctt ccaatagcaa atgtgagcag
     5941 aatcatgaag aaagctctac cagcaaacgc gaaaatatca aaggacgcta aagagatagt
     6001 tcaagaatgt gtttctgaat tcatcagttt cattactggg gaagcatcag acaagtgtca
     6061 gcgcgagaag aggaaaacca tcaacggtga tgatttgttg tgggcaatga caacacttgg
     6121 ttttgaagag tacattgagc cccttaagat ttatctgcag aggttcaggg acttagaagg
     6181 gcaaaagagc accatggtag gaatacaaga caaagagaat agtggatcag tgaatatggg
     6241 tagtagttat gttgacgact atggcatgat gatgatgggt cagcatcatc aaggacacac
     6301 gtatggtact ggagtataca atcagacggc tggaaatgat gcagggattg ataatgcagg
     6361 gtctcgattt cctgatgttg ggaggccaag gcccggggac ccagctttct tgtacaaagt
     6421 ggttgataac agcgggttaa ttaacatctt ttacccatac gatgttcctg actatgcggg
     6481 ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc cagattacgc
     6541 tgctcagtgc agcgcttaga gctcgaattt ccccgatcgt tcaaacattt ggcaataaag
     6601 tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa
     6661 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     6721 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     6781 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatcggg aattagcttc
     6841 atcaacgcaa gacatgcgca cgaccgtctg acaggagagg aatttccgac gagcacagaa
     6901 aggacttgct cttggacgta ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg
     6961 ggttttcgat ggtgtatcag ccgccgccaa ctgggagatg aggaggcttt cttggggggc
     7021 agtcagcagt tcatttcaca agacagagga acttgtaagg agatgcactg atttatcttg
     7081 gcgcaaacca gcaggacgaa ttagtgggaa tagcccgcga atatctaagt tatgcctgtc
     7141 ggcatgagca gaaacttcca attcgaaaca gtttggagag gttgtttttg ggcatacctt
     7201 ttgttagtca gcctctcgat tgctcatcgt cattacacag taccgaagtt tgatcgatct
     7261 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
     7321 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
     7381 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
     7441 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
     7501 gaacgatctg cttcgacgca ctccttcttt actccaccat ctcgtcctta ttgaaaacgt
     7561 gggtagcacc aaaacgaatc aagtcgctgg aactgaagtt accaatcacg ctggatgatt
     7621 tgccagttgg attaatcttg cctttccccg catgaataat attgatgaat gcatgcgtga
     7681 ggggtatttc gattttggca atagctgcaa ttgccgcgac atcctccaac gagcataatt
     7741 cttcagaaaa atagcgatgt tccatgttgt cagggcatgc atgatgcacg ttatgaggtg
     7801 acggtgctag gcagtattcc ctcaaagttt catagtcagt atcatattca tcattgcatt
     7861 cctgcaagag agaattgaga cgcaatccac acgctgcggc aaccttccgg cgttcgtggt
     7921 ctatttgctc ttggacgttg caaacgtaag tgttggatcc cggtcggcat ctactctatt
     7981 cctttgccct cggacgagtg ctggggcgtc ggtttccact atcggcgagt acttctacac
     8041 agccatcggt ccagacggcc gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg
     8101 ctccggatcg gacgattgcg tcgcatcgac cctgcgccca agctgcatca tcgaaattgc
     8161 cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg gagcatatac gcccggagcc
     8221 gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac
     8281 aagccaacca cggcctccag aagaagatgt tggcgacctc gtattgggaa tccccgaaca
     8341 tcgcctcgct ccagtcaatg accgctgtta tgcggccatt gtccgtcagg acattgttgg
     8401 agccgaaatc cgcgtgcacg aggtgccgga cttcggggca gtcctcggcc caaagcatca
     8461 gctcatcgag agcctgcgcg acggacgcac tgacggtgtc gtccatcaca gtttgccagt
     8521 gatacacatg gggatcagca atcgcgcata tgaaatcacg ccatgtagtg tattgaccga
     8581 ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg
     8641 catccatggc ctccgcgacc ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt
     8701 gcaacgtgac accctgtgca cggcgggaga tgcaataggt caggctctcg ctgaattccc
     8761 caatgtcaag cacttccgga atcgggagcg cggccgatgc aaagtgccga taaacataac
     8821 gatctttgta gaaaccatcg gcgcagctat ttacccgcag gacatatcca cgccctccta
     8881 catcgaagct gaaagcacga gattcttcgc cctccgagag ctgcatcagg tcggagacgc
     8941 tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc ggtgagttca ggctttttca
     9001 tatcggggtc gtcctctcca aatgaaatga acttccttat atagaggaag ggtcttgcga
     9061 aggatagtgg gattgtgcgt catcccttac gtcagtggag atatcacatc aatccacttg
     9121 ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc
     9181 atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg
     9241 atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc
     9301 tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc
     9361 cctttggtct tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc
     9421 caccatgttg acggatctct aggacgcgtc gacgcgtcga cgcgtcgacg cgtcgacgcg
     9481 tcctagaagc taattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     9541 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     9601 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc
     9661 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     9721 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     9781 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     9841 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     9901 atctcgggct attcttttga tttataaggg attttgccga tttcggaacc accatcaaac
     9961 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
    10021 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag
    10081 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
    10141 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
    10201 ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg
    10261 cggcagactt tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt
    10321 ttgaaacacg gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg
    10381 ctgcctgtga tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca
    10441 tttctcgctt aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat
    10501 cgctggataa agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac
    10561 gatatcaact cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg
    10621 actgtcggcc tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag
    10681 taaggaaaca actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa
    10741 acccgctccg atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg
    10801 gattggcgga tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg
    10861 ccaggcggta aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc
    10921 gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc
    10981 gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc
    11041 catcaatcgt atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg
    11101 cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa
    11161 gctccagaat agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc
    11221 cgttggaatc tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag
    11281 cataccggcg acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc
    11341 cttgtgagcg tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc
    11401 gtcgatgtag gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg
    11461 ctttttctcc tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga
    11521 caatcggatc tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt
    11581 aatcacaatt gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt
    11641 cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc
    11701 agtaaagcgc tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg
    11761 caatgcacca ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct
    11821 tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac
    11881 atgaggcgga aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg
    11941 aacatccgtc gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag
    12001 tggtcgccag caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt
    12061 ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca
    12121 acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc
    12181 ttcgtgtaat accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg
    12241 aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc
    12301 agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg
    12361 acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg
    12421 aacagggcag agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca
    12481 atatcgaaca aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg
    12541 gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg
    12601 gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt
    12661 tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt
    12721 tgcacgctgt cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg
    12781 aaggtttcgc ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg
    12841 gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt
    12901 caccctcctt gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt
    12961 gacccgcctg gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg
    13021 tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc
    13081 agcgacccct tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg
    13141 cggaagaagt cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta
    13201 aaacaattca tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag
    13261 taagtcaaaa aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac
    13321 gcagaaggca atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc
    13381 acttactttg ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac
    13441 aagttcctct tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa
    13501 tggagtgtct tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta
    13561 atccaattcg gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat
    13621 ggagtgaaag agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc
    13681 atcttcatac tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca
    13741 gccatcatgc cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag
    13801 catcatgtcc ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat
    13861 ttttaaatat aggttttcat tttctcccac cagcttatat accttagcag gagacattcc
    13921 ttccgtatct tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct
    13981 cattttagcc atttattatt tccttcctct tttctacagt atttaaagat accccaagaa
    14041 gctaattata acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac
    14101 cagaaaacag ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag
    14161 ccgattttga aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg
    14221 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
    14281 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac
    14341 tgccgtaaaa catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag
    14401 aaaatcattg atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc
    14461 gttggcctca acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc
    14521 gcatacagcc gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg
    14581 tcggggctaa atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg
    14641 ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc
    14701 tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc
    14761 tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc
    14821 tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg
    14881 tggagctgca tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg
    14941 gagtcattac ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa
    15001 gcattagtcc atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc
    15061 tgcctatgcc ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat
    15121 attatcttat cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc
    15181 tcttcgtctt ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa
    15241 ttctcgtttt catacctcgg tataatctta cctatcacct caaatggttc gctgggttta
    15301 tcgcaccccc gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa
    15361 aaattgccgg ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc
    15421 cgccacccag gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca
    15481 cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg
    15541 ccccgatccc ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg
    15601 cggccgaggg gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt
    15661 cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg
    15721 ttaaaaacaa ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg
    15781 ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca
    15841 aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc
    15901 gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc
    15961 aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc
    16021 gaggctggcc agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc
    16081 gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc
    16141 aagttttccg cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac
    16201 ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc
    16261 agcccggtga gcgtcggaaa ggcgc
//
