LOCUS       sequence-247494-       15946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15946)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX140
FEATURES             Location/Qualifiers
     source          1..15946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6060..6084)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6113..6202
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6236..6488
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6917..7169)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7637..8662)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7637..8662)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8672..9016)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9158..9175)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9158..9174)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9167..9189)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9314..9333)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9524..9545
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9792..9816
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11412..12560)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12859..13653)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13845..14612
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15305..15936
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatggc caatgatgtc gaagctaaag gattcaaccc
     5881 aggcttgatc gtcttacttc ttattggtgg gctcgtgttg gcgtttctta ttggaaacta
     5941 tgtcctttat gtgtatgccc agaaaaccct tcctccaaag aaaaagaagc cactctccaa
     6001 gaagaagatg aagagggaaa ggctgaagca aggtgtatcg gcacccggag agcccgggga
     6061 cccagctttc ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata
     6121 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
     6181 atatgacgtt ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg
     6241 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     6301 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6361 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6421 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6481 actagatcgg gaattagctt catcaacgca agacatgcgc acgaccgtct gacaggagag
     6541 gaatttccga cgagcacaga aaggacttgc tcttggacgt aggcctattt ctcaggcaca
     6601 tgtatcaagt gttcggacgt gggttttcga tggtgtatca gccgccgcca actgggagat
     6661 gaggaggctt tcttgggggg cagtcagcag ttcatttcac aagacagagg aacttgtaag
     6721 gagatgcact gatttatctt ggcgcaaacc agcaggacga attagtggga atagcccgcg
     6781 aatatctaag ttatgcctgt cggcatgagc agaaacttcc aattcgaaac agtttggaga
     6841 ggttgttttt gggcatacct tttgttagtc agcctctcga ttgctcatcg tcattacaca
     6901 gtaccgaagt ttgatcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     6961 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     7021 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     7081 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     7141 aaactttatt gccaaatgtt tgaacgatct gcttcgacgc actccttctt tactccacca
     7201 tctcgtcctt attgaaaacg tgggtagcac caaaacgaat caagtcgctg gaactgaagt
     7261 taccaatcac gctggatgat ttgccagttg gattaatctt gcctttcccc gcatgaataa
     7321 tattgatgaa tgcatgcgtg aggggtattt cgattttggc aatagctgca attgccgcga
     7381 catcctccaa cgagcataat tcttcagaaa aatagcgatg ttccatgttg tcagggcatg
     7441 catgatgcac gttatgaggt gacggtgcta ggcagtattc cctcaaagtt tcatagtcag
     7501 tatcatattc atcattgcat tcctgcaaga gagaattgag acgcaatcca cacgctgcgg
     7561 caaccttccg gcgttcgtgg tctatttgct cttggacgtt gcaaacgtaa gtgttggatc
     7621 ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     7681 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     7741 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     7801 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     7861 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     7921 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     7981 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     8041 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     8101 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     8161 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     8221 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     8281 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
     8341 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     8401 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     8461 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     8521 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     8581 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     8641 cggtgagttc aggctttttc atatcggggt cgtcctctcc aaatgaaatg aacttcctta
     8701 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     8761 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     8821 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     8881 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
     8941 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     9001 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     9061 gtagacgaga gtgtcgtgct ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg
     9121 acgcgtcgac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
     9181 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9241 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9301 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9361 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9421 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     9481 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9541 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     9601 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9661 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9721 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     9781 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     9841 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     9901 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     9961 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
    10021 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
    10081 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
    10141 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
    10201 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
    10261 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
    10321 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
    10381 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    10441 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    10501 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    10561 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    10621 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    10681 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    10741 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    10801 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    10861 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    10921 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    10981 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    11041 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    11101 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    11161 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    11221 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    11281 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    11341 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    11401 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    11461 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    11521 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    11581 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    11641 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    11701 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    11761 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    11821 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    11881 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    11941 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    12001 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    12061 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    12121 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    12181 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    12241 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    12301 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    12361 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    12421 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    12481 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    12541 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    12601 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    12661 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    12721 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    12781 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    12841 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    12901 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    12961 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    13021 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    13081 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    13141 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13201 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    13261 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    13321 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    13381 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13441 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    13501 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    13561 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    13621 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    13681 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13741 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    13801 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    13861 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    13921 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    13981 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    14041 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    14101 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    14161 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    14221 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    14281 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    14341 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    14401 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    14461 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    14521 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    14581 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    14641 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    14701 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    14761 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    14821 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    14881 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    14941 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    15001 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    15061 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    15121 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    15181 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    15241 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    15301 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    15361 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    15421 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    15481 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    15541 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    15601 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    15661 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    15721 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    15781 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    15841 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    15901 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
