LOCUS       sequence-247499-       15458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15458)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX142
FEATURES             Location/Qualifiers
     source          1..15458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5604..5628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5657..5746
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5780..6032
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6461..6713)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7181..8206)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7181..8206)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8216..8560)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8670..8687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8670..8686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8679..8701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8826..8845)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9036..9057
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9304..9328
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10924..12072)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12371..13165)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13357..14124
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14817..15448
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggaatc
     4621 atcatcatca tcatcctcct catctaagag ggcaaaagca cctggcaata ttgctcaagt
     4681 agctcattgc ttggttgatg gatgtaatgc agacctcagt caatgcagag agtatcaccg
     4741 ccgccataaa gtttgtgagg tccattcaaa gactgccaaa gtcaccattg cgggtcgaga
     4801 ccaacgcttc tgtcagcaat gcagcaggtt tcattccttg gtagagtttg atgatggaaa
     4861 gagaagctgc cggaaacgtc ttgatggaca taacaggcgt cgaaggaaac ctcagcctga
     4921 ttctatggcc agaacttctg gattactttt taccggccag caagggacga aacttctgtc
     4981 atttagcagt caacaaattt ttccaagtgc agctgcatgg gctggcgtag tcaaaacgga
     5041 cagcgacatg gtattataca acaaccaatc gcatataaat tgcatggaca atcaaaactc
     5101 gttccctgat tcttcagctt gtagctacaa aggaggaaat caattccagt tcatgcaagg
     5161 cagtgaccat agtctccctg aagcttcaat ctgccagcca cttgttgatc atgccaattc
     5221 tgcagcagca gcagtttcta gcagcggaca aaagatcttc tccagtggat tgaacgaaat
     5281 tgtggactct gatcgtgctc tctctcttct gtcatcagca cccgctgtaa ctagggagat
     5341 tggttttagt cacatggtgc agcagcctgc ttctatcccc cggccccagt cacaatccgt
     5401 cgctcatagc ctacactatg gtggtctaag ccagtaccct tttgctcaag atttcaatag
     5461 ccaacctcaa gattctgctg cagattcaca tgttagcaac aacagctctc tgcatttcca
     5521 tgatatgtta caaaatggac cagatggatc atctacaagt gctggctgtg agcaaacact
     5581 agcctttatg tgggaccccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     5641 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     5701 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     5761 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     5821 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     5881 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     5941 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     6001 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     6061 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     6121 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     6181 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     6241 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     6301 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     6361 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     6421 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     6481 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     6541 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     6601 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     6661 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     6721 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     6781 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     6841 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     6901 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     6961 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     7021 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     7081 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     7141 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     7201 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7261 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7321 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7381 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     7441 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     7501 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     7561 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     7621 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     7681 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     7741 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     7801 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     7861 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7921 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     7981 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     8041 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     8101 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     8161 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     8221 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8281 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8341 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8401 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8461 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8521 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8581 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     8641 tctctaggac gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg
     8701 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     8761 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     8821 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8881 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8941 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     9001 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     9061 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga
     9121 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa
     9181 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga
     9241 aaaaccaccc cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa
     9301 tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct
     9361 cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag
     9421 agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac
     9481 taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga
     9541 tgacagagcg ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca
     9601 gccttcttat tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga
     9661 cataatagga aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga
     9721 agtgaacgtt gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg
     9781 acccgaagtt ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc
     9841 tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa
     9901 ggaagtaggt taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc
     9961 ctgtaggcat cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc
    10021 cggccgccag ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg
    10081 tcagcacggt tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag
    10141 gatctagcgc tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag
    10201 cccccaggac cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga
    10261 ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg
    10321 aaataaacaa caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca
    10381 tccaccagat tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca
    10441 acgcccgcag cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc
    10501 cggacagatg cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc
    10561 caatgatctc gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga
    10621 acgcctccat gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt
    10681 tcttcagggc cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc
    10741 gaatctgagc cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga
    10801 gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg
    10861 ttcctgaaat gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag
    10921 gccctagcgt ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct
    10981 gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga
    11041 atccgatccg cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga
    11101 gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat
    11161 gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg
    11221 gcaacgggac gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga
    11281 ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag
    11341 gcattcctcg gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag
    11401 ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac
    11461 ctgctgccac accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt
    11521 cacgtccttg ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt
    11581 gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc
    11641 cggccacggc gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg
    11701 tttcagcaac gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt
    11761 ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc
    11821 tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc
    11881 agggggagcc agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga
    11941 gccgacggac tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc
    12001 ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa
    12061 cgtccgattc attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc
    12121 ttattcctga tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat
    12181 gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc
    12241 ctgcacgaat accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc
    12301 aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca
    12361 catctaggta ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag
    12421 gcttgatccc cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc
    12481 tgatcgaccg gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa
    12541 gatcaataaa gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc
    12601 cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc
    12661 gcggatcttt aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt
    12721 tattcagtaa gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat
    12781 ccgatatgtc gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt
    12841 cagggctttg ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga
    12901 gcagattgct ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc
    12961 cttccagcca tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc
    13021 ggctgtccgt catttttaaa tataggtttt cattttctcc caccagctta tataccttag
    13081 caggagacat tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt
    13141 ccggtgatat tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa
    13201 gataccccaa gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta
    13261 aaaccttaaa taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat
    13321 agtatcgacg gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt
    13381 tagtgtatga tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc
    13441 ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta
    13501 tctctgctct cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc
    13561 cggtacgcac cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc
    13621 ccgcattatg ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag
    13681 tcggtaacct cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa
    13741 cagtggggct atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt
    13801 ctccggaaga cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt
    13861 cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg
    13921 tatgaatccc gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt
    13981 gagcggcata acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg
    14041 ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac
    14101 tatcaataag ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg
    14161 tcctgggttt caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata
    14221 ttgataagcg cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct
    14281 atataaaaga tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc
    14341 atcctcttca tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc
    14401 tgtaaaggtc caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg
    14461 ttcgctgggt ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga
    14521 atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa
    14581 gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat
    14641 gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc
    14701 catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg
    14761 gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg
    14821 ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag
    14881 ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac
    14941 aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt
    15001 ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag
    15061 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag
    15121 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt
    15181 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc
    15241 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct
    15301 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc
    15361 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc
    15421 ggcgagggca accagcccgg tgagcgtcgg aaaggcgc
//
