LOCUS       sequence-247503-       15553 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15553)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX144
FEATURES             Location/Qualifiers
     source          1..15553
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             4703..4720
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(5667..5691)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5720..5809
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5843..6095
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6524..6776)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7244..8269)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7244..8269)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8279..8623)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8765..8782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8765..8781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8774..8796)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8921..8940)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9131..9152
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9399..9423
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11019..12167)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12466..13260)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13452..14219
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14912..15543
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgtggtc
     4621 tgcatcgtca tcccgacaaa tgcagatcaa ctatggcctc tcacctgaaa tgggtatgtt
     4681 tgtagtacat cccgcttcat ttcaccacca ccatcatcat caacattcac atcaagaaaa
     4741 taccattaat tttgacccga taaacggctc caatatctct acaacgaaca catctcttgg
     4801 tgttggtgta attccgcttc tcactgcaac tcctttaacg aacatggtta gcttcgacga
     4861 tcaagattta gtgagtagaa atcgaggaaa tgatactgaa ggtggtggtt ttcagttctt
     4921 ttcaaaccaa caacctcaga actctacgaa ttataccaaa aacagtaaca ttcttggtag
     4981 tggcaatgac aacaacattg gtggttcagt ttcatcaaca agttcagcaa catgtcaaga
     5041 ttgtggaaac caagctaaaa aggattgtcc acacagaaga tgtagaacat gttgtaaaag
     5101 tagaggtttt gattgtacaa cacatgtaaa aagtacttgg gtaccagcgg cgagaaggcg
     5161 cgagagacaa ctaattggag gcacttcaac gaatgtaaat gttgctgctg gttcatcttc
     5221 tcactcaact tcaagtgcta agaaacctag acttgttaat tctcaagcta caactacagc
     5281 ttctcatact tctacttcaa atactactcc tagaagtttt gatactagtt ctagtcatca
     5341 agatgcaagt tttaaagcct cattgccagg acaagtgaga gcaccagctg tattcaagtg
     5401 tgtaagagtg acttcagtgg atgatggaga tgatgaattt gcatatcaag ctgttgttag
     5461 aattggtggc catgttttca aaggttttct atatgatcaa ggaattgaag aaggcaaaga
     5521 ttataataat tatcccaatt tatctgattt acatttgggt ggcagtggta atattactag
     5581 tgaaacttct gatatttatg ctgcttctgc tgctggtggt ggattacttg gtggatcaaa
     5641 ttatgttaac caaataaatc ccggggaccc agctttcttg tacaaagtgg ttgataacag
     5701 cgggttaatt aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga
     5761 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag
     5821 cgcttagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt
     5881 gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca
     5941 tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt
     6001 cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa
     6061 attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga
     6121 catgcgcacg accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct
     6181 tggacgtagg cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg
     6241 tgtatcagcc gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc
     6301 atttcacaag acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc
     6361 aggacgaatt agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga
     6421 aacttccaat tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc
     6481 ctctcgattg ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat
     6541 gacaccgcgc gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat
     6601 taaatgtata attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat
     6661 tacatgttaa ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca
     6721 agaccggcaa caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct
     6781 tcgacgcact ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa
     6841 aacgaatcaa gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat
     6901 taatcttgcc tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga
     6961 ttttggcaat agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat
     7021 agcgatgttc catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc
     7081 agtattccct caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag
     7141 aattgagacg caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt
     7201 ggacgttgca aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg
     7261 gacgagtgct ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc
     7321 agacggccgc gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga
     7381 cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc
     7441 tctgatagag ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg
     7501 caagctccgg atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg
     7561 gcctccagaa gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc
     7621 agtcaatgac cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg
     7681 cgtgcacgag gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag
     7741 cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg
     7801 gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc
     7861 cgaatgggcc gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct
     7921 ccgcgaccgg ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac
     7981 cctgtgcacg gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca
     8041 cttccggaat cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga
     8101 aaccatcggc gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga
     8161 aagcacgaga ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt
     8221 cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt
     8281 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     8341 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     8401 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     8461 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     8521 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     8581 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     8641 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac
     8701 ggatctctag gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc ctagaagcta
     8761 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     8821 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     8881 atcgcccttc ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct
     8941 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     9001 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     9061 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     9121 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat
     9181 tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc
     9241 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     9301 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac
     9361 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     9421 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     9481 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     9541 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     9601 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     9661 aaatatcatc tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa
     9721 ccgtgacagg ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag
     9781 ccgctgagga agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc
     9841 cctatccatt gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg
     9901 atgcatcccc ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac
     9961 tgtaggttcg agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat
    10021 caggccgagc cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc
    10081 aaacactaaa gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa
    10141 ggtgagcaga ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga
    10201 aaccgccccc gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa
    10261 caccaacagc gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat
    10321 cgggctacct agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac
    10381 cgtcgccgcg accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag
    10441 cgaaatatta agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg
    10501 tcggacgatc atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac
    10561 ccctcggcct cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc
    10621 cttggggccg tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc
    10681 gccgaatgcc acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc
    10741 gtgctcgtaa acggacccga acatctctgg agctttcttc agggccgaca atcggatctc
    10801 gcggaaatcc tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt
    10861 caattttaat cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac
    10921 tgagcgaagc aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg
    10981 gctgctgaac ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg
    11041 tcatcattga cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg
    11101 ccgacctgct cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag
    11161 gtttccagct tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg
    11221 gccgtcggcg acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca
    11281 aacagcacga cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg
    11341 tccaggacgc ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac
    11401 gtgaagccca tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac
    11461 cggccattga tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc
    11521 tcgccgatag gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg
    11581 tcggcccgca gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg
    11641 accttgtttt gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag
    11701 cgggccgtgt cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag
    11761 gaaagctgca tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc
    11821 tcgctgacct gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt
    11881 cctcgcgtgt cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc
    11941 gaacgctcca cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg
    12001 cgctcgatct tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg
    12061 ggcgcacgca tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg
    12121 tcgatcagtt cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc
    12181 gggattgccc cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt
    12241 gccttggtgt ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg
    12301 ccgtccttct cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg
    12361 cccaaatact tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg
    12421 gtgcgctcct gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc
    12481 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
    12541 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
    12601 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
    12661 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
    12721 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
    12781 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
    12841 taagcggctg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
    12901 cctgatgcac tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc
    12961 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
    13021 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
    13081 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
    13141 gttttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
    13201 tacgcagcgg tatttttcga tcagtttttt caattccggt gatattctca ttttagccat
    13261 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
    13321 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct
    13381 ttttcaaagt tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa
    13441 ccacaattat gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt
    13501 gctccagtgg cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg
    13561 cgtaacggca aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca
    13621 tggcaactgc agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat
    13681 atggccatga atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac
    13741 acgattttac gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg
    13801 gcagtgacgt catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat
    13861 cgcgccagcg ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg
    13921 tattcggtga acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg
    13981 acgtggtgat atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc
    14041 tgcacgtaat cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc
    14101 agcacctggc acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg
    14161 acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc
    14221 aattatgata gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat
    14281 gcaagttttt atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt
    14341 gccccctgaa atccttacat acggcgatat cttctatata aaagatatat tatcttatca
    14401 gtattgtcaa tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg
    14461 tagcttttta aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca
    14521 tacctcggta taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga
    14581 acacgagcac ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc
    14641 ccgccatgaa gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc
    14701 cgccgccctc actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt
    14761 caatgcttcc gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg
    14821 caatggcaag gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc
    14881 gcagcccctg gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg
    14941 gcacccccct tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg
    15001 tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg
    15061 cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg
    15121 tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg
    15181 tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac
    15241 atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag
    15301 ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag
    15361 tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg
    15421 aggtatccac aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc
    15481 gcgtttgcag ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc
    15541 gtcggaaagg cgc
//
