LOCUS       sequence-247504-       15526 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15526)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX145
FEATURES             Location/Qualifiers
     source          1..15526
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5664..5688)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5717..5806
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5840..6092
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6521..6773)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7241..8266)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7241..8266)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8276..8620)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8738..8755)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8738..8754)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8747..8769)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8894..8913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9104..9125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9372..9396
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10992..12140)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12439..13233)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13425..14192
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14885..15516
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggctgg
     4621 gttcttttca ctaggtggag gagctgctag tagcagccaa gcccaagacc agcagcacca
     4681 acaaaacacc aataatcctc ccactgaaat tagtccagaa agttggttct tgtacagaaa
     4741 tgatcaagag ttacctacat acagaggctt tgagctatgg caaactagta attcccagcc
     4801 tcagattcgg catccagtta atccgcttca agatctttat cccaccgccg ctgttggatt
     4861 aggcgtcggg ccgagcctca gtgactttac tatgactgca gctcgccccg atcatgaccc
     4921 ttcttctggg ggattggtca tgatgagaag tgggggaggt ggaattagct gccaagattg
     4981 tgggaaccaa gcgaagaaag attgtgaaca catgagatgt aggacttgtt gcaagagccg
     5041 aggctttcaa tgccaaaccc atgtgaaaag cacttgggtt ccagcggcta aaaggagaga
     5101 aaggcaacaa caacttacta ctttacagca gcagcaagaa gaacaaaagc agcagcaaca
     5161 gcaaacgcgg cagctgcaaa tagataataa ccccaaaagg caaagagagg atccaagttc
     5221 ttcctctctt gtttgcactc gtatattacc ttctagtact tctgggttag aagtggggaa
     5281 ttttccatcc aaagtaagtt caactgccgt atttcactgc gttcggatga gttccgtaga
     5341 tgatgctgag gatcaattcg cttatcaaac agctgtgaac attggtggac acgtatttaa
     5401 agggatttta tacgatcaag gtcctgagaa tcattacatg gctgctggtg aaagctcgtc
     5461 cgccggtgga agtgctagtg accaccataa tcttattggt gctgcaggca ccgccacctc
     5521 agcagccacg acttctgcga gtggtggcgg tgtagctgca gcacccgaag cctcacctta
     5581 tctcgaccct tctttatatc cagctcccat caacactttc atggctggta cgcaattctt
     5641 tccacctcca agatctcccg gggacccagc tttcttgtac aaagtggttg ataacagcgg
     5701 gttaattaac atcttttacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     5761 cccggactat gcaggatcct atccatatga cgttccagat tacgctgctc agtgcagcgc
     5821 ttagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa
     5881 tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt
     5941 aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc
     6001 gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt
     6061 atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta gcttcatcaa cgcaagacat
     6121 gcgcacgacc gtctgacagg agaggaattt ccgacgagca cagaaaggac ttgctcttgg
     6181 acgtaggcct atttctcagg cacatgtatc aagtgttcgg acgtgggttt tcgatggtgt
     6241 atcagccgcc gccaactggg agatgaggag gctttcttgg ggggcagtca gcagttcatt
     6301 tcacaagaca gaggaacttg taaggagatg cactgattta tcttggcgca aaccagcagg
     6361 acgaattagt gggaatagcc cgcgaatatc taagttatgc ctgtcggcat gagcagaaac
     6421 ttccaattcg aaacagtttg gagaggttgt ttttgggcat accttttgtt agtcagcctc
     6481 tcgattgctc atcgtcatta cacagtaccg aagtttgatc gatctagtaa catagatgac
     6541 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     6601 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     6661 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     6721 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atctgcttcg
     6781 acgcactcct tctttactcc accatctcgt ccttattgaa aacgtgggta gcaccaaaac
     6841 gaatcaagtc gctggaactg aagttaccaa tcacgctgga tgatttgcca gttggattaa
     6901 tcttgccttt ccccgcatga ataatattga tgaatgcatg cgtgaggggt atttcgattt
     6961 tggcaatagc tgcaattgcc gcgacatcct ccaacgagca taattcttca gaaaaatagc
     7021 gatgttccat gttgtcaggg catgcatgat gcacgttatg aggtgacggt gctaggcagt
     7081 attccctcaa agtttcatag tcagtatcat attcatcatt gcattcctgc aagagagaat
     7141 tgagacgcaa tccacacgct gcggcaacct tccggcgttc gtggtctatt tgctcttgga
     7201 cgttgcaaac gtaagtgttg gatcccggtc ggcatctact ctattccttt gccctcggac
     7261 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     7321 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     7381 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     7441 gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa
     7501 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     7561 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     7621 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     7681 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     7741 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     7801 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     7861 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg
     7921 cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7981 gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt
     8041 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     8101 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     8161 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     8221 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg gggtcgtcct
     8281 ctccaaatga aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg
     8341 tgcgtcatcc cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt
     8401 tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact
     8461 gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt
     8521 gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc
     8581 gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga
     8641 gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca tgttgacgga
     8701 tctctaggac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
     8761 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     8821 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     8881 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8941 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9001 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     9061 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9121 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     9181 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9241 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9301 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     9361 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     9421 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     9481 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     9541 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     9601 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
     9661 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
     9721 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
     9781 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
     9841 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
     9901 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
     9961 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    10021 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    10081 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    10141 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    10201 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    10261 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    10321 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    10381 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    10441 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    10501 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    10561 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    10621 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    10681 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    10741 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    10801 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    10861 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    10921 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    10981 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    11041 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    11101 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    11161 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    11221 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    11281 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    11341 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    11401 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    11461 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    11521 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    11581 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    11641 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    11701 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    11761 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    11821 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    11881 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    11941 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    12001 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    12061 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    12121 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    12181 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    12241 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    12301 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    12361 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    12421 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    12481 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    12541 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    12601 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    12661 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    12721 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    12781 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    12841 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    12901 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    12961 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13021 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    13081 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    13141 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    13201 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    13261 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13321 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    13381 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    13441 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    13501 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    13561 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    13621 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    13681 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    13741 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    13801 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    13861 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    13921 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    13981 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    14041 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    14101 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    14161 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    14221 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    14281 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    14341 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    14401 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    14461 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    14521 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    14581 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    14641 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    14701 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    14761 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    14821 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    14881 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    14941 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    15001 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    15061 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    15121 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    15181 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    15241 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    15301 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    15361 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    15421 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    15481 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
