LOCUS       sequence-247520-       15088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15088)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX151
FEATURES             Location/Qualifiers
     source          1..15088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5202..5226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5255..5344
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5378..5630
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6059..6311)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6779..7804)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(6779..7804)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(7814..8158)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8300..8317)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8300..8316)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8309..8331)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8456..8475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8666..8687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    8934..8958
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10554..11702)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12001..12795)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  12987..13754
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14447..15078
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatgycray
     4621 kkykkkwgmm gctarsgkyt yymmrctcac tcgcgaacta ggtcacaagt ccgacggtca
     4681 aaccattgaa tggctgctgc gtcaagctga accctcgatt gtcgccgcca ctggtactgg
     4741 tactatacct gctagctttt caacactctc tgtttcgctg cggaactctt cagtttcttc
     4801 ttctgtttcc gctccgcttg attacaagtc gtcatcacaa ccgtcgattt cgccggcgcc
     4861 gtttttgctc gggaagcgtc tgcggcacga ggatgacgac gggagtgggg aaaaagatga
     4921 cgctagtaac ggagctacct ttgtgggacc tacccctggg ttttgggctg ttgcagctag
     4981 gccggatttt ggccaggttt ggagctttgc gccgccaccg ccgcaggaga tggtggcgcc
     5041 gacaccttca tcggcagcga gatttcttca gcaacaaatg ggagaggaat cggctgctag
     5101 attagggaat tactttccta tagctcaagg gcacctgaat ttgttagctt ctttatcggg
     5161 tccttcttct gggagaagag acgatgacgg aaaccccggg gacccagctt tcttgtacaa
     5221 agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc ctgactatgc
     5281 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
     5341 cgctgctcag tgcagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     5401 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     5461 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     5521 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     5581 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattagc
     5641 ttcatcaacg caagacatgc gcacgaccgt ctgacaggag aggaatttcc gacgagcaca
     5701 gaaaggactt gctcttggac gtaggcctat ttctcaggca catgtatcaa gtgttcggac
     5761 gtgggttttc gatggtgtat cagccgccgc caactgggag atgaggaggc tttcttgggg
     5821 ggcagtcagc agttcatttc acaagacaga ggaacttgta aggagatgca ctgatttatc
     5881 ttggcgcaaa ccagcaggac gaattagtgg gaatagcccg cgaatatcta agttatgcct
     5941 gtcggcatga gcagaaactt ccaattcgaa acagtttgga gaggttgttt ttgggcatac
     6001 cttttgttag tcagcctctc gattgctcat cgtcattaca cagtaccgaa gtttgatcga
     6061 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     6121 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     6181 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     6241 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     6301 tttgaacgat ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa
     6361 cgtgggtagc accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg
     6421 atttgccagt tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg
     6481 tgaggggtat ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata
     6541 attcttcaga aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag
     6601 gtgacggtgc taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc
     6661 attcctgcaa gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt
     6721 ggtctatttg ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg catctactct
     6781 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     6841 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     6901 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     6961 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     7021 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     7081 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     7141 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     7201 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     7261 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     7321 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     7381 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     7441 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     7501 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     7561 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     7621 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     7681 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     7741 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     7801 tcatatcggg gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     7861 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     7921 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     7981 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     8041 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     8101 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     8161 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     8221 ctccaccatg ttgacggatc tctaggacgc gtcswcgcrk cgamgyswcr wckcgtcgac
     8281 gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8341 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8401 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct
     8461 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8521 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8581 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8641 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     8701 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca
     8761 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     8821 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     8881 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     8941 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     9001 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     9061 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     9121 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     9181 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
     9241 tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga
     9301 aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt
     9361 gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt
     9421 ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc
     9481 cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt
     9541 taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat
     9601 cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag
     9661 ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt
     9721 tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc
     9781 tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac
     9841 cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg
     9901 ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa
     9961 caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat
    10021 tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag
    10081 cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg
    10141 cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc
    10201 gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat
    10261 gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc
    10321 cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc
    10381 cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg
    10441 cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat
    10501 gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt
    10561 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
    10621 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
    10681 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
    10741 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
    10801 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
    10861 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
    10921 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
    10981 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
    11041 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
    11101 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
    11161 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
    11221 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
    11281 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
    11341 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
    11401 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
    11461 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
    11521 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac
    11581 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
    11641 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
    11701 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
    11761 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
    11821 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
    11881 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
    11941 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta
    12001 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    12061 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    12121 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    12181 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    12241 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    12301 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    12361 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    12421 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    12481 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    12541 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    12601 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    12661 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    12721 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    12781 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    12841 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    12901 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    12961 gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga
    13021 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
    13081 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct
    13141 cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac
    13201 cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg
    13261 ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct
    13321 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct
    13381 atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga
    13441 cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc
    13501 tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
    13561 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata
    13621 acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat
    13681 cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag
    13741 ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt
    13801 caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg
    13861 cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga
    13921 tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca
    13981 tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc
    14041 caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt
    14101 ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg
    14161 taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca
    14221 ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca
    14281 gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta
    14341 ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt
    14401 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
    14461 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
    14521 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
    14581 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
    14641 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
    14701 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
    14761 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
    14821 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
    14881 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
    14941 gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc
    15001 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
    15061 accagcccgg tgagcgtcgg aaaggcgc
//
