LOCUS       sequence-247527-        1021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of histone H3.3K4M-FLAG/HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1021)
  AUTHORS   Jang Y, Broun A, Wang C, Park YK, Zhuang L, Lee JE, Froimchuk E, Liu
            C, Ge K
  TITLE     H3.3K4M destabilizes enhancer H3K4 methyltransferases MLL3/MLL4 and 
            impairs adipose tissue development.
  JOURNAL   Nucleic Acids Res. 2019 Jan 25;47(2):607-620. doi: 
            10.1093/nar/gky982.
  PUBMED    30335158
REFERENCE   2  (bases 1 to 1021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-01-25- 25"; volume: "47"; issue: 
            "2"; pages: "607-620"
COMMENT     Sequence Label: pBT346.6-H3.3-K4M-bGHpolyA
FEATURES             Location/Qualifiers
     source          1..1021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..44
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             534..557
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             573..599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             573..599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(665..683)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(666..683)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(703..720)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    709..820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cggancttat ataacttcgt ataatgtatg ctatacgaag ttattaggtc cctcgacctg
       61 ctgcagctcg aggcatgcgt cgacgtttct agtctagact cgagtgaacc atggcccgaa
      121 ccatgcagac tgctcgtaag tccaccggtg ggaaagcccc ccgcaaacag ctggccacga
      181 aagccgccag gaaaagcgct ccctctaccg gcggggtgaa gaagcctcat cgctacaggc
      241 ccgggaccgt ggcgcttcga gagattcgtc gttatcagaa gtcgaccgag ctgctcatcc
      301 ggaagctgcc cttccagagg ttggtgaggg agatcgcgca ggatttcaaa accgacctga
      361 ggtttcagag cgcagccatc ggtgcgctgc aggaggctag cgaagcgtac ctggtgggtc
      421 tgttcgaaga taccaacctg tgtgccatcc acgctaagag agtcaccatc atgcccaaag
      481 acatccagtt ggctcgccgg atacggggag agagagctgc ggccgctgga ggagactaca
      541 aggacgacga tgacaagtcg gccgctggag gataccccta cgacgtgccc gactacgcct
      601 aggaattcgt taacgaaatc catcacactg gcggccgctc gagcatgcat ctagagggcc
      661 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
      721 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaanntgcca
      781 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtnntgtca
      841 ttctatctgg ggggnngggg nnggggcagg acagcaaggg gggaggattg ggaanacata
      901 ncagcatgct ggggatgcgt gnctctatgg cgcgcccaac tagttctaga gcgcncacag
      961 tttgtacaaa aagctgacga naangtaaat gatatnaata tcannnnnta atnanatttt
     1021 g
//
