LOCUS       sequence-247532-         492 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of histone H3.3WT-FLAG/HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Jang Y, Broun A, Wang C, Park YK, Zhuang L, Lee JE, Froimchuk E, Liu
            C, Ge K
  TITLE     H3.3K4M destabilizes enhancer H3K4 methyltransferases MLL3/MLL4 and 
            impairs adipose tissue development.
  JOURNAL   Nucleic Acids Res. 2019 Jan 25;47(2):607-620. doi: 
            10.1093/nar/gky982.
  PUBMED    30335158
REFERENCE   2  (bases 1 to 492)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2019-01-25- 25"; volume: "47"; issue: 
            "2"; pages: "607-620"
COMMENT     Sequence Label: pMSCVhyg-H3.3WT-FLAG/HA
FEATURES             Location/Qualifiers
     source          1..492
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             424..447
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             463..489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             463..489
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 atggcccgaa ccaagcagac tgctcgtaag tccaccggtg ggaaagcccc ccgcaaacag
       61 ctggccacga aagccgccag gaaaagcgct ccctctaccg gcggggtgaa gaagcctcat
      121 cgctacaggc ccgggaccgt ggcgcttcga gagattcgtc gttatcagaa gtcgaccgag
      181 ctgctcatcc ggaagctgcc cttccagagg ttggtgaggg agatcgcgca ggatttcaaa
      241 accgacctga ggtttcagag cgcagccatc ggtgcgctgc aggaggctag cgaagcgtac
      301 ctggtgggtc tgttcgaaga taccaacctg tgtgccatcc acgctaagag agtcaccatc
      361 atgcccaaag acatccagtt ggctcgccgg atacggggag agagagctgc ggccgctgga
      421 ggagactaca aggacgacga tgacaagtcg gccgctggag gataccccta cgacgtgccc
      481 gactacgcct ag
//
