LOCUS       sequence-247549-       15407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15407)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX153
FEATURES             Location/Qualifiers
     source          1..15407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5553..5577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5606..5695
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5729..5981
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6410..6662)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7130..8155)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7130..8155)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8165..8509)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8619..8636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8619..8635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8628..8650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8775..8794)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8985..9006
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9253..9277
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10873..12021)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12320..13114)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13306..14073
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14766..15397
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggagag
     4621 agatttcatg ggactcaact ctaaagattc tgttgttgtg gtcaaggaag aacctgttga
     4681 aacctgcaaa gactctgggt ttcgctggcc gttgtcaagc aaggtgggta tacctcaatt
     4741 catgtcttgg aattctgctc aagatgaaaa accgctcaaa gctcaatccg ctgcagatgg
     4801 agtggacagt tgtctcaaac gtcagtctgg tgaaatccag atatctgcag ctgcgacaat
     4861 gacgcagcaa cttcttgggg gaatccctgt cacagctcct cattcaatac ttccatcgag
     4921 tggctctgtg gctgggataa ctgaaccttg gtttaattcc aaaggttctg cagcacctgc
     4981 tcaactgacc atcttctatg gcgggacggt caatgtcttt gatgatattt cccctgagaa
     5041 ggcacaggct attatgtttt tggctggaaa tggttgcgtt cctcctaatg tggtgcaacc
     5101 aaggtttcaa gttcaggcat ctacacccaa acttgctgct gtagacggca cttgtgtgaa
     5161 ccaaacccca aacatgcttc ctgcgtcagg tcattccagc cctatgtctg tttcttctca
     5221 tcccattggt caatctgctg gcaattctgg aaacaaagat gacatgaaga tatctaaaac
     5281 tgcaaacatt tcagtggaaa ctccaaagat tatgacatca ctaggacctg ttggggcgac
     5341 taccataatg ccagcagcgg ttccacaggc taggaaagca tctctggctc ggtttctgga
     5401 gaagcggaag gaaagggtga tgaacgctgc accatatggc ctcagcaaga aatcgggtga
     5461 gtgctccacc cccgagtcta acggagttgg tttctctgca acttccagtg ttggtactag
     5521 tcctctgata gccggtaagg agacccccgg ggacccagct ttcttgtaca aagtggttga
     5581 taacagcggg ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     5641 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     5701 gtgcagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     5761 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5821 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     5881 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     5941 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattag cttcatcaac
     6001 gcaagacatg cgcacgaccg tctgacagga gaggaatttc cgacgagcac agaaaggact
     6061 tgctcttgga cgtaggccta tttctcaggc acatgtatca agtgttcgga cgtgggtttt
     6121 cgatggtgta tcagccgccg ccaactggga gatgaggagg ctttcttggg gggcagtcag
     6181 cagttcattt cacaagacag aggaacttgt aaggagatgc actgatttat cttggcgcaa
     6241 accagcagga cgaattagtg ggaatagccc gcgaatatct aagttatgcc tgtcggcatg
     6301 agcagaaact tccaattcga aacagtttgg agaggttgtt tttgggcata ccttttgtta
     6361 gtcagcctct cgattgctca tcgtcattac acagtaccga agtttgatcg atctagtaac
     6421 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     6481 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     6541 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     6601 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     6661 tctgcttcga cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag
     6721 caccaaaacg aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag
     6781 ttggattaat cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta
     6841 tttcgatttt ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag
     6901 aaaaatagcg atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg
     6961 ctaggcagta ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca
     7021 agagagaatt gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt
     7081 gctcttggac gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg
     7141 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     7201 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     7261 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     7321 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     7381 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     7441 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     7501 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     7561 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     7621 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     7681 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     7741 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     7801 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     7861 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     7921 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     7981 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     8041 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     8101 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg
     8161 ggtcgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     8221 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     8281 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     8341 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     8401 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     8461 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     8521 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     8581 gttgacggat ctctaggacg cgtcctagaa gctaattcac tggccgtcgt tttacaacgt
     8641 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     8701 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     8761 ctgaatggcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8821 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8881 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     8941 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     9001 aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc
     9061 gatttcggaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     9121 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     9181 gtgaaaagaa aaaccacccc agtacattaa aaacgtccgc aatgtgttat taagttgtct
     9241 aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga
     9301 ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt
     9361 cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag
     9421 aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga
     9481 ttgtaacgat gacagagcgt tgctgcctgt gatcaaatat catctccctc gcagagatcc
     9541 gaattatcag ccttcttatt catttctcgc ttaaccgtga caggctgtcg atcttgagaa
     9601 ctatgccgac ataataggaa atcgctggat aaagccgctg aggaagctga gtggcgctat
     9661 ttctttagaa gtgaacgttg acgatatcaa ctcccctatc cattgctcac cgaatggtac
     9721 aggtcgggga cccgaagttc cgactgtcgg cctgatgcat ccccggctga tcgaccccag
     9781 atctggggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc
     9841 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa
     9901 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca
     9961 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc
    10021 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga
    10081 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc
    10141 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga
    10201 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc
    10261 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga
    10321 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac
    10381 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca
    10441 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga
    10501 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc
    10561 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct
    10621 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc
    10681 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca
    10741 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt
    10801 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga
    10861 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac
    10921 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac
    10981 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc
    11041 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt
    11101 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat
    11161 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag
    11221 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag
    11281 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc
    11341 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta
    11401 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta
    11461 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg
    11521 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg
    11581 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg
    11641 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc
    11701 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt
    11761 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc
    11821 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg
    11881 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc
    11941 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt
    12001 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc
    12061 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt
    12121 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg
    12181 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc
    12241 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc
    12301 gttgcgccac atctaggtac taaaacaatt catccagtaa aatataatat tttattttct
    12361 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
    12421 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
    12481 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
    12541 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
    12601 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
    12661 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
    12721 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
    12781 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
    12841 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
    12901 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca taggtggtcc
    12961 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
    13021 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
    13081 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
    13141 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
    13201 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
    13261 gtataacata gtatcgacgg agccgatttt gaaaccacaa ttatgggtga tgctgccaac
    13321 ttactgattt agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag
    13381 ctgtccctcc tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca
    13441 tcagcgctat ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc
    13501 ttctcaaccc ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg
    13561 ggcaacagcc cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc
    13621 aggccgcagt cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa
    13681 atggacgaac agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg
    13741 tatgacagtc tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg
    13801 ctggggcgtc ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc
    13861 tggccgctgt atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg
    13921 cagcgaattg agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag
    13981 tcgctgtcgt tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac
    14041 ataaaacact atcaataagt tggagtcatt acccaattat gatagaattt acaagctata
    14101 aggttattgt cctgggtttc aagcattagt ccatgcaagt ttttatgctt tgcccattct
    14161 atagatatat tgataagcgc gctgcctatg ccttgccccc tgaaatcctt acatacggcg
    14221 atatcttcta tataaaagat atattatctt atcagtattg tcaatatatt caaggcaatc
    14281 tgcctcctca tcctcttcat cctcttcgtc ttggtagctt tttaaatatg gcgcttcata
    14341 gagtaattct gtaaaggtcc aattctcgtt ttcatacctc ggtataatct tacctatcac
    14401 ctcaaatggt tcgctgggtt tatcgcaccc ccgaacacga gcacggcacc cgcgaccact
    14461 atgccaagaa tgcccaaggt aaaaattgcc ggccccgcca tgaagtccgt gaatgccccg
    14521 acggccgaag tgaagggcag gccgccaccc aggccgccgc cctcactgcc cggcacctgg
    14581 tcgctgaatg tcgatgccag cacctgcggc acgtcaatgc ttccgggcgt cgcgctcggg
    14641 ctgatcgccc atcccgttac tgccccgatc ccggcaatgg caaggactgc cagcgctgcc
    14701 atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga tgggaggccc
    14761 gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg tgcgcggtca
    14821 cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt taaaagcagg
    14881 ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt
    14941 tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg
    15001 cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc tcaagtgtca
    15061 ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac tcgcgtaaaa
    15121 tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc gaaatcgagc
    15181 ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt caacgtccgc
    15241 ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc ggcggccgcg
    15301 gtgtctcgca cacggcttcg acggcgtttc tggcgcgttt gcagggccat agacggccgc
    15361 cagcccagcg gcgagggcaa ccagcccggt gagcgtcgga aaggcgc
//
