LOCUS       sequence-247555-       11340 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology 
            arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression 
            after integration. SA-GFP cassette contains PGK-puromycin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11340)
  TITLE     Karpf Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAVS1-SAGFP
FEATURES             Location/Qualifiers
     source          1..11340
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    23..859
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(878..898)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(878..896)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(917..933)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(917..933)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(930..952)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    941..957
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(965..971,972..989,990..995))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1010..1031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1148..1165)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1319..1907)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1399..1418)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2078..2869,2870..2938))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2701..2720
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2939..3043)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3069..3524)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3206..3227)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3418..3437
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3651..3673
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3665..3682
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3666..3682
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3692..3711
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3692..3710
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3733..4536
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    4543..4568
                     /label=SA
                     /note="splice acceptor site"
     CDS             4592..4645
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             4655..5680
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             4655..5680
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(5712..5729)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5718..5942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    5949..6030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5974..5993
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     enhancer        6050..6429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          6707..7723
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7647..7669
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7731..7750
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      7778..7787
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7784..7786,7787..7789,7790..8302)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(7829..7850)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        8540..9037
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8575..8598)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8970..8989
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9056..9655
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9056..9075)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9552..9572
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(10113..10132)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    10178..10233
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(10232..10251)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     11166..11187
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattgattat gcatccctcg agactaggga caggattggt gacagaaaag ccccatcctt
       61 aggcctcctc cttcctagtc tcctgatatt gggtctaacc cccacctcct gttaggcaga
      121 ttccttatct ggtgacacac ccccatttcc tggagccatc tctctccttg ccagaacctc
      181 taaggtttgc ttacgatgga gccagagagg atcctgggag ggagagcttg gcagggggtg
      241 ggagggaagg gggggatgcg tgacctgccc ggttctcagt ggccaccctg cgctaccctc
      301 tcccagaacc tgagctgctc tgacgcggct gtctggtgcg tttcactgat cctggtgctg
      361 cagcttcctt acacttccca agaggagaag cagtttggaa aaacaaaatc agaataagtt
      421 ggtcctgagt tctaactttg gctcttcacc tttctagtcc ccaatttata ttgttcctcc
      481 gtgcgtcagt tttacctgtg agataaggcc agtagccagc cccgtcctgg cagggctgtg
      541 gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga gaatggtgcg tcctaggtgt
      601 tcaccaggtc gtggccgcct ctactccctt tctctttctc catccttctt tccttaaaga
      661 gtccccagtg ctatctggga catattcctc cgcccagagc agggtcccgc ttccctaagg
      721 ccctgctctg ggcttctggg tttgagtcct tggcaagccc aggagaggcg ctcaggcttc
      781 cctgtccccc ttcctcgtcc accatctcat gcccctggct ctcctgcccc ttccctacag
      841 gggttcctgg ctctgctctg gtacccagct tttgttccct ttagtgaggg ttaattgcgc
      901 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      961 cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     1021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     1081 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     1141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     1201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     1681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     1741 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     1801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     1861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     1921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     1981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     2041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     2101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     2161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     2221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     2281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     2341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     2401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     2461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     2521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     2581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     2641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     2701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     2761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     2821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     2881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     2941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     3001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     3061 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     3121 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     3181 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     3241 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     3301 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     3361 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     3421 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     3481 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
     3541 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     3601 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     3661 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattgga
     3721 gctccaccgc ggtgctttct ctgaccagca ttctctcccc tgggcctgtg ccgctttctg
     3781 tctgcagctt gtggcctggg tcacctctac ggctggccca gatccttccc tgccgcctcc
     3841 ttcaggttcc gtcttcctcc actccctctt ccccttgctc tctgctgtgt tgctgcccaa
     3901 ggatgctctt tccggagcac ttccttctcg gcgctgcacc acgtgatgtc ctctgagcgg
     3961 atcctccccg tgtctgggtc ctctccgggc atctctcctc cctcacccaa ccccatgccg
     4021 tcttcactcg ctgggttccc ttttccttct ccttctgggg cctgtgccat ctctcgtttc
     4081 ttaggatggc cttctccgac ggatgtctcc cttgcgtccc gcctcccctt cttgtaggcc
     4141 tgcatcatca ccgtttttct ggacaacccc aaagtacccc gtctccctgg ctttagccac
     4201 ctctccatcc tcttgctttc tttgcctgga caccccgttc tcctgtggat tcgggtcacc
     4261 tctcactcct ttcatttggg cagctcccct acccccctta cctctctagt ctgtgctagc
     4321 tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta gtcttcttcc
     4381 tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc agggatcctg
     4441 tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct ctggttctgg
     4501 gtacttttat ctgtcccctc caccccacag tggggcagat ccctgacctc ttctcttcct
     4561 cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc taacatgcgg
     4621 tgacgtggag gagaatcccg gccctaggct gcagatgaaa aagcctgaac tcaccgcgac
     4681 gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc
     4741 ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg
     4801 ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc
     4861 ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta
     4921 ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc
     4981 cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca
     5041 gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga
     5101 tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac
     5161 cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc
     5221 cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg
     5281 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt
     5341 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt
     5401 cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat
     5461 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc
     5521 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat
     5581 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg
     5641 aaaccgacgc cccagcactc gtccgagggc aaaggaatag tctagagggc ccgtttaaac
     5701 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     5761 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     5821 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     5881 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     5941 gggtcgaggc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6001 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attctggtcg acattgatta
     6061 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     6121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     6181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     6241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     6301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     6361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     6421 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
     6481 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
     6541 gggggggggg sgcgcgccrg gsggggsggg gsggggsgrg gggsggggsg gggsgrggcg
     6601 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
     6661 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
     6721 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
     6781 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
     6841 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
     6901 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt
     6961 ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc
     7021 ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     7081 gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg
     7141 agcagggggt gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt
     7201 tgctgagcac ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc
     7261 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     7321 cggggrgggc tcgggggagg ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc
     7381 ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg gacttccttt
     7441 gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg
     7501 cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc
     7561 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
     7621 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
     7681 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
     7741 ggttattgtg ctgtctcatc attttggcaa agaattcgcc accatggtga gcaagggcga
     7801 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     7861 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     7921 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     7981 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     8041 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     8101 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     8161 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     8221 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     8281 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     8341 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     8401 cgccctgagc aaagacccca acgagaagtg agaattcact cctcaggtgc aggctgccta
     8461 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctgat
     8521 atcatcgatg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     8581 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcacacattc
     8641 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     8701 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     8761 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     8821 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     8881 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     8941 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     9001 cgcgctgttc tcctcttcct catctccggg cctttcgacc gatccagccg ccaccatgac
     9061 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     9121 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     9181 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     9241 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     9301 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     9361 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     9421 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     9481 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     9541 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     9601 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     9661 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag
     9721 ccacccgggg cggccccgcc gaccccgcac ccgcccccga ggcccaccgc gggggacaca
     9781 ccgaacacgc cgaccctgct gaacacgcgg cgcagttcgg tgcccaggag cggatcgaaa
     9841 ttgatgatct attaaacaat aaagatgtcc actaaaatgg aagtttttcc tgtcatactt
     9901 tgttaagaag ggtgagaaca gagtacctac attttgaatg gaaggattgg agctacgggg
     9961 gtgggggtgg ggtgggatta gataaatgcc tgctctttac tgaaggctct ttactattgc
    10021 tttatgataa tgtttcatag ttggatatca taatttaaac aagcaaaacc aaattaaggg
    10081 ccagctcatt cctcccactc atgatctata gatctttttc cctctgccaa aaattatggg
    10141 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
    10201 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
    10261 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
    10321 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
    10381 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
    10441 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
    10501 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
    10561 ctcgacctgc agcccaagct ttaaggccac aagttcagcg tgtccggcga gggcgagggc
    10621 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
    10681 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
    10741 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
    10801 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgat
    10861 agggataaca gggtaatccg catcgagctg aagggcatcg acttcaagga ggacggcaac
    10921 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
    10981 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
    11041 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
    11101 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
    11161 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
    11221 ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtggagg
    11281 ttttacttgc ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
//
