LOCUS       sequence-247556-       17345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17345)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 17345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX156
FEATURES             Location/Qualifiers
     source          1..17345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7459..7483)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7512..7601
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7635..7887
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8316..8568)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(9036..10061)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9036..10061)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(10071..10415)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10557..10574)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10557..10573)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10566..10588)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10713..10732)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10923..10944
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11191..11215
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12811..13959)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14258..15052)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15244..16011
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16704..17335
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat ggcagcttct tcaacaatca tagacacatc ctctatgcag
     5881 aattcaagtt tctcattcac tgatcttctt gcttctgatg attatccaac aaagagcaaa
     5941 ggacttggtg ataggattgc agagagaact ggttctggaa ttcccaaatt taagtcactt
     6001 ccacctcctt cattacccct ttctccgcct cctttttctc cttcatctta ctttgctatt
     6061 cctcctggtt taagccctac tgaactcttg gactctcctc ttcttttgtc tgcttccaac
     6121 attcttccat ctccaacaac agggacattt ccagctcagg cttttaactg gaagagcagt
     6181 tccaatagca gccatcaggg tgtcaaacag gaagacaaaa actactctga tttctctttc
     6241 cagccccaat ttggacaatc ttttgcatca gtctcttcgt cccaaactaa tcttgtccct
     6301 ttggggaaac aatcatggaa ttatcaagaa tcaagaaagc agaatgatca atcatctgaa
     6361 aatgctaatc aaagaactga attcaatact atacagaact tgaaaaataa tgctcagagc
     6421 aaccaatata acaaccaatc atcacagagt ataagggagc agaaaagagc agaagatggg
     6481 tacaattgga ggaaatatgg acagaaacaa gtgaagggaa gtgaaaatcc aagaagttac
     6541 tataaatgta ctttcccaaa ttgtcccaca aagaaaaaag tagagaggtc tttagatgga
     6601 caaattactg aaattgtata caagggtaat cacaaccatc caaagcctca gtctactagg
     6661 agatcctcat ccacagcttc ctctttagcc gtccagcctt acaatacaca aattaatgaa
     6721 atcccagatc atcaatcata tggttcaaat gcaacacctg aaaattcttc catttcattt
     6781 ggtgatgatg atcatgatcg tgagcaaagc tctcaaaaga gtaggtcaag aggagatgat
     6841 ttcgacgacg aagaagaacc agactcaaaa agatggaaaa gagaaagcga aagtgaaatt
     6901 ttgtcagcac caggaagcag aacagtaaga gagcctagag ttgtagttca aacaacaagt
     6961 gatattgata ttctagatga tggttataga tggagaaagt atggtcaaaa ggtagtgaaa
     7021 ggaaatccta atccaaggag ctactacaaa tgcacgagcc aaggatgtcc agtaagaaaa
     7081 catgtggaac gagcatcaca agacataaga tcagtgataa caacttatga aggaaaacac
     7141 aaccatgatg ttccagcagc taggggcagt ggcataaacc gacctgtcgc accaaccatt
     7201 acttataaca atggtgccaa taatgccatg gtcataaggc cttctgtcac atctcaaatt
     7261 ccattacaaa gtataaggcc gcaacagtct ccatttacac tagagatgtt gcagaagcct
     7321 agtaattata atggtttctc aggatatgca aattcagagg attcatatga aaaccaactt
     7381 caggacaata atgtattttc cagagcaaag gatgagcctc gagatgacat gtttatggag
     7441 acattgctct gctcgaggac ccagctttct tgtacaaagt ggttgataac agcgggttaa
     7501 ttaacatctt ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     7561 actatgcagg atcctatcca tatgacgttc cagattacgc tgctcagtgc agcgcttaga
     7621 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     7681 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     7741 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     7801 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     7861 gcgcggtgtc atctatgtta ctagatcggg aattagcttc atcaacgcaa gacatgcgca
     7921 cgaccgtctg acaggagagg aatttccgac gagcacagaa aggacttgct cttggacgta
     7981 ggcctatttc tcaggcacat gtatcaagtg ttcggacgtg ggttttcgat ggtgtatcag
     8041 ccgccgccaa ctgggagatg aggaggcttt cttggggggc agtcagcagt tcatttcaca
     8101 agacagagga acttgtaagg agatgcactg atttatcttg gcgcaaacca gcaggacgaa
     8161 ttagtgggaa tagcccgcga atatctaagt tatgcctgtc ggcatgagca gaaacttcca
     8221 attcgaaaca gtttggagag gttgtttttg ggcatacctt ttgttagtca gcctctcgat
     8281 tgctcatcgt cattacacag taccgaagtt tgatcgatct agtaacatag atgacaccgc
     8341 gcgcgataat ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta
     8401 taattgcggg actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt
     8461 aattattaca tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc
     8521 aacaggattc aatcttaaga aactttattg ccaaatgttt gaacgatctg cttcgacgca
     8581 ctccttcttt actccaccat ctcgtcctta ttgaaaacgt gggtagcacc aaaacgaatc
     8641 aagtcgctgg aactgaagtt accaatcacg ctggatgatt tgccagttgg attaatcttg
     8701 cctttccccg catgaataat attgatgaat gcatgcgtga ggggtatttc gattttggca
     8761 atagctgcaa ttgccgcgac atcctccaac gagcataatt cttcagaaaa atagcgatgt
     8821 tccatgttgt cagggcatgc atgatgcacg ttatgaggtg acggtgctag gcagtattcc
     8881 ctcaaagttt catagtcagt atcatattca tcattgcatt cctgcaagag agaattgaga
     8941 cgcaatccac acgctgcggc aaccttccgg cgttcgtggt ctatttgctc ttggacgttg
     9001 caaacgtaag tgttggatcc cggtcggcat ctactctatt cctttgccct cggacgagtg
     9061 ctggggcgtc ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc
     9121 gcgcttctgc gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg
     9181 tcgcatcgac cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag
     9241 agttggtcaa gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc
     9301 ggatgcctcc gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag
     9361 aagaagatgt tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg
     9421 accgctgtta tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg
     9481 aggtgccgga cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg
     9541 acggacgcac tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca
     9601 atcgcgcata tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg
     9661 ccgaacccgc tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc
     9721 ggctgcagaa cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca
     9781 cggcgggaga tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga
     9841 atcgggagcg cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg
     9901 gcgcagctat ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga
     9961 gattcttcgc cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga
    10021 aacttctcga cagacgtcgc ggtgagttca ggctttttca tatcggggtc gtcctctcca
    10081 aatgaaatga acttccttat atagaggaag ggtcttgcga aggatagtgg gattgtgcgt
    10141 catcccttac gtcagtggag atatcacatc aatccacttg ctttgaagac gtggttggaa
    10201 cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga ccactgtcgg
    10261 cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg taggtgccac
    10321 cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg aatccgagga
    10381 ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct tctgagactg
    10441 tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg acggatctct
    10501 aggacgcgtc gacgcgtcga cgcgtcgacg cgtcgacgcg tcctagaagc taattcactg
    10561 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    10621 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    10681 tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc gctttcttcc cttcctttct
    10741 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10801 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
    10861 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    10921 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga
    10981 tttataaggg attttgccga tttcggaacc accatcaaac aggattttcg cctgctgggg
    11041 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
    11101 ctgttgcccg tctcactggt gaaaagaaaa accaccccag tacattaaaa acgtccgcaa
    11161 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
    11221 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
    11281 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
    11341 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
    11401 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaaatatca
    11461 tctccctcgc agagatccga attatcagcc ttcttattca tttctcgctt aaccgtgaca
    11521 ggctgtcgat cttgagaact atgccgacat aataggaaat cgctggataa agccgctgag
    11581 gaagctgagt ggcgctattt ctttagaagt gaacgttgac gatatcaact cccctatcca
    11641 ttgctcaccg aatggtacag gtcggggacc cgaagttccg actgtcggcc tgatgcatcc
    11701 ccggctgatc gaccccagat ctggggctga gaaagcccag taaggaaaca actgtaggtt
    11761 cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg atcaggccga
    11821 gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga tcaaacacta
    11881 aagctactgg aacgagcaga agtcctccgg ccgccagttg ccaggcggta aaggtgagca
    11941 gaggcacggg aggttgccac ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc
    12001 ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca
    12061 gcgccacgcc cgcagttccg caaatagccc ccaggaccgc catcaatcgt atcgggctac
    12121 ctagcagagc ggcagagatg aacacgacca tcagcggctg cacagcgcct accgtcgccg
    12181 cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa gctccagaat agcgaaatat
    12241 taagtgcgcc gaggatgaag atgcgcatcc accagattcc cgttggaatc tgtcggacga
    12301 tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg acccctcggc
    12361 ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg tccttggggc
    12421 cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag gcgccgaatg
    12481 ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc tcgtgctcgt
    12541 aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc tcgcggaaat
    12601 cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt gtcaatttta
    12661 atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa
    12721 gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga
    12781 acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt
    12841 gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg
    12901 ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga aggtttccag
    12961 cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg
    13021 cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag caaacagcac
    13081 gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac ggtccaggac
    13141 gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg acgtgaagcc
    13201 catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat accggccatt
    13261 gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat
    13321 aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat cgtcggcccg
    13381 cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt
    13441 ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt
    13501 gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg
    13561 catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac
    13621 ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt
    13681 gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc
    13741 cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt cgcgctcgat
    13801 cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg
    13861 catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag
    13921 ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt gcgggattgc
    13981 cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg gtgccttggt
    14041 gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt
    14101 ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct tgcccaaata
    14161 cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt cggtgcgctc
    14221 ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca tccagtaaaa
    14281 tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga
    14341 catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc
    14401 acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca
    14461 caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt
    14521 ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt
    14581 tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc
    14641 tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc
    14701 actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc
    14761 aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt
    14821 gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt
    14881 ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat
    14941 tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc
    15001 ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt
    15061 tccttcctct tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa
    15121 ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa
    15181 gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt
    15241 atgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag gtgctccagt
    15301 ggcttctgtt tctatcagct gtccctcctg ttcagctact gacggggtgg tgcgtaacgg
    15361 caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa catggcaact
    15421 gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg atatggccat
    15481 gaatggcgtt ggatgccggg caacagcccg cattatgggc gttggcctca acacgatttt
    15541 acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc gggcagtgac
    15601 gtcatcgtct gcgcggaaat ggacgaacag tggggctatg tcggggctaa atcgcgccag
    15661 cgctggctgt tttacgcgta tgacagtctc cggaagacgg ttgttgcgca cgtattcggt
    15721 gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg
    15781 atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta
    15841 atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg
    15901 gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc
    15961 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac ccaattatga
    16021 tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc atgcaagttt
    16081 ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc ttgccccctg
    16141 aaatccttac atacggcgat atcttctata taaaagatat attatcttat cagtattgtc
    16201 aatatattca aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt ggtagctttt
    16261 taaatatggc gcttcataga gtaattctgt aaaggtccaa ttctcgtttt catacctcgg
    16321 tataatctta cctatcacct caaatggttc gctgggttta tcgcaccccc gaacacgagc
    16381 acggcacccg cgaccactat gccaagaatg cccaaggtaa aaattgccgg ccccgccatg
    16441 aagtccgtga atgccccgac ggccgaagtg aagggcaggc cgccacccag gccgccgccc
    16501 tcactgcccg gcacctggtc gctgaatgtc gatgccagca cctgcggcac gtcaatgctt
    16561 ccgggcgtcg cgctcgggct gatcgcccat cccgttactg ccccgatccc ggcaatggca
    16621 aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc
    16681 tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc
    16741 cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa
    16801 tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc
    16861 cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc
    16921 tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt
    16981 cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg
    17041 tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt
    17101 cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc
    17161 tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc
    17221 acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc
    17281 agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcggaaa
    17341 ggcgc
//
