LOCUS       sequence-247563-       10182 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting vector for the human AAVS1 locus for insertion of traffic 
            light reporter (TLR) construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Chu VT, Weber T, Wefers B, Wurst W, Sander S, Rajewsky K, Kuhn R
  TITLE     Increasing the efficiency of homology-directed repair for 
            CRISPR-Cas9-induced precise gene editing in mammalian cells.
  JOURNAL   Nat Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198.
  PUBMED    25803306
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 Mar 24. doi: 10.1038/nbt.3198."
COMMENT     Sequence Label: pAAVS1-TLR targeting vector
FEATURES             Location/Qualifiers
     source          1..10182
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    295..1098
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1105..1130
                     /label=SA
                     /note="splice acceptor site"
     CDS             1154..1207
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1217..1816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1217..1236)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1713..1733
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    1854..2078
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        2142..2521
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2523..2798
                     /label=chicken beta-actin promoter
     intron          2799..3815
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3739..3761
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     regulatory      3831..3840
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3882..3903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4143..4162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             4302..4553
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4490..4511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4565..4621
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4622..5335
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     complement(5361..5377)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5397..5621
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    5692..6528
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(6554..6573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6555..6573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6580..6597)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6580..6596)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6589..6611)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             6734..7036
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6915..6934
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             7385..8179
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7385..8179
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7439..7458)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8049..8068
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(8429..9220,9221..9289))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9052..9071
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9413..10001
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9902..9921
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10155..10172
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt
      301 ctctgaccag cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg
      361 ggtcacctct acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct
      421 ccactccctc ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc
      481 acttccttct cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg
      541 tcctctccgg gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc
      601 ccttttcctt ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg
      661 acggatgtct cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt
      721 ctggacaacc ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt
      781 tctttgcctg gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg
      841 ggcagctccc ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca
      901 tggcatcttc caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat
      961 gtccacttca ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc
     1021 accttatatt cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc
     1081 tccaccccac agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag
     1141 atctggcagc ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc
     1201 cggccctagg ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     1261 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     1321 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     1381 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     1441 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     1501 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     1561 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     1621 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     1681 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     1741 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     1801 caagcccggt gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     1861 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1921 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1981 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2041 caatagcagg catgctgggg atgcggtggg ctctatgggt cgacggtatc gataagcttg
     2101 atatcgaatt cgagctcggt acccgggggc gcgccggatt cgacattgat tattgactag
     2161 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
     2221 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
     2281 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
     2341 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
     2401 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
     2461 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
     2521 ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc
     2581 aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg
     2641 ggcgcgcgcc aggggggggg gggggggggg gggggggggg gggggggggg cggagaggtg
     2701 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
     2761 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
     2821 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
     2881 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
     2941 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
     3001 gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg
     3061 ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt
     3121 gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     3181 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3241 gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc
     3301 acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg
     3361 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     3421 gctcggggga ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3481 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3541 tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga
     3601 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     3661 gccgtcccct tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg
     3721 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
     3781 ctaaccatgt tcatgccttc ttctttttcc tacagatcct taattaagcc gccaccatgg
     3841 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3901 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3961 agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4021 tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc
     4081 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4141 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagctgcaa ctccagtctt
     4201 tctagaagat gggcgggagt cttctgggca ggcttatatc aagcgctatg tgcaccaaaa
     4261 cttctcctcg cactaccggg ccaccattgg tgatcaccgc cgacaagcag aagaacggca
     4321 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc
     4381 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4441 tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4501 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtagacgc
     4561 gttggccacg aacttctctc tgttaaagca agcaggagat gttgaagaaa accccgggcc
     4621 tatggtgtct aagggcgaag agctgattaa ggagaacatg cacatgaagc tgtacatgga
     4681 gggcaccgtg aacaaccacc acttcaagtg cacatccgag ggcgaaggca agccctacga
     4741 gggcacccag accatgagaa tcaaggtggt cgagggcggc cctctcccct tcgccttcga
     4801 catcctggct accagcttca tgtacggcag cagaaccttc atcaaccaca cccagggcat
     4861 ccccgacttc tttaagcagt ccttccctga gggcttcaca tgggagagag tcaccacata
     4921 cgaagacggg ggcgtgctga ccgctaccca ggacaccagc ctccaggacg gctgcctcat
     4981 ctacaacgtc aagatcagag gggtgaactt cccatccaac ggccctgtga tgcagaagaa
     5041 aacactcggc tgggaggcca acaccgagat gctgtacccc gctgacggcg gcctggaagg
     5101 cagaagcgac atggccctga agctcgtggg cgggggccac ctgatctgca acttcaagac
     5161 cacatacaga tccaagaaac ccgctaagaa cctcaagatg cccggcgtct actatgtgga
     5221 ccacagactg gaaagaatca aggaggccga caaagagacc tacgtcgagc agcacgaggt
     5281 ggctgtggcc agatactgcg acctccctag caaactgggg cacaaactta attgagcgat
     5341 cgcacgcgta aatgattgca gatccactag ttctagagct cgctgatcag cctcgactgt
     5401 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     5461 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     5521 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     5581 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     5641 cagctggggc tcgagatcca ctagttctag cctcgaggct agagcggccg cactagggac
     5701 aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct cctgatattg
     5761 ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc cccatttcct
     5821 ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag ccagagagga
     5881 tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt gacctgcccg
     5941 gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct gacgcggctg
     6001 tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa gaggagaagc
     6061 agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg ctcttcacct
     6121 ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga gataaggcca
     6181 gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc
     6241 cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc tactcccttt
     6301 ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac atattcctcc
     6361 gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt ttgagtcctt
     6421 ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg
     6481 cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctctaa gggcggccgc
     6541 taaattcaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     6601 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     6661 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     6721 cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt
     6781 tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga tccccctggc
     6841 cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg tgcatatcgg
     6901 ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg
     6961 ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat
     7021 gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca cctagatcct
     7081 tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     7141 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     7201 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     7261 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     7321 gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat gaggatcgtt
     7381 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     7441 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     7501 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     7561 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     7621 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     7681 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     7741 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     7801 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     7861 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     7921 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     7981 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     8041 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     8101 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     8161 tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt attttctcct
     8221 tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga aatgtgcgcg
     8281 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagattat
     8341 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8401 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8461 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8521 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8581 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8641 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8701 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8761 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8821 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8881 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8941 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga atactcaacc aagtcattct
     9001 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     9061 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9121 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9181 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9241 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9301 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gaccaaaatc ccttaacgtg
     9361 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     9421 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     9481 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     9541 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     9601 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     9661 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     9721 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     9781 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     9841 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     9901 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     9961 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
    10021 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
    10081 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
    10141 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
