LOCUS       sequence-247566-       15766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15766)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX159
FEATURES             Location/Qualifiers
     source          1..15766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4562..4586
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5880..5904)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5933..6022
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6056..6308
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6737..6989)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7457..8482)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7457..8482)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8492..8836)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8978..8995)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8978..8994)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8987..9009)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9134..9153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9344..9365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9612..9636
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11232..12380)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12679..13473)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13665..14432
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15125..15756
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gacaagtttg tacaaaaaag caggctccac cactagtatg tcgttccgca gtatagttcg
     4621 ggatgtaaga gatagctttg gcagcttatc caggcgaagc tttgacgtaa ggctctctgg
     4681 tcatcaaagg ggtaaatcac acggttcatt tcatgactta agcgatcagc ctgttgttgt
     4741 ccagaacagt tgttgggcta atctcccgcc agaactactt tttgatgtaa ttggaagatt
     4801 agaagagagt gagaacacat ggcctgctcg taagcatgtt gtagcatgtg cttcagtttg
     4861 caggtcatgg aggattatgt gccaggaaat tgtaagaagt cctgaagttt gtggaaagct
     4921 tactttcccg gtgtcgttaa agcagcctgg gcctcgtgac ggaatgattc aatgcttcat
     4981 caagagggat aaatctaatt tgacttacca tcttttcttg tgtctcagtc ctgctttact
     5041 agttgaaaat gggaagttcc ttctctctgc aaagagaaca aggcggacta cttgcacgga
     5101 gtatgttatc tccatgaatg cagaaaacat ctcgagatca agcaacactt acgttggaaa
     5161 attaaggtca aattttctgg gtacaaaatt cgttatatat gacacacagc ctccatgcac
     5221 atccgcgaac atccccccgc cttgccgaac aagccgtaga ttctccaaga aagtctctcc
     5281 taaagtccca actggaagct acagcatagc tcagatcaaa tacgagctaa atgttcttgg
     5341 aacaaggggc ccacggaaaa tgaactgtgt catgcattca attcctgcct cagcacttga
     5401 tgttggcggt tctgtgcctg gccaaccaga gcttctctct aggtcccttg aggattcatt
     5461 ccgaagtatc tctttctcaa agtcgcttga tcattctact gatttcagta gctcacggtt
     5521 ttcggaactt gttagagcat catccaacga gggagatgat agcaacacga aacccttggt
     5581 tctaaagaac aagtctccgc gttggcatga acaactgcag tgctggtgcc taaactttcg
     5641 aggacgagtg acagttgcat ctgtaaagaa ttttcagttg attgccgcca accaaccacc
     5701 tgctggcggg cccacaacct ctcggtcaag tcagtcagat catgacaaag tcatcctgca
     5761 attcggaaag ataggcaaag acatgttcac tatggattac aggtatccac tgtctgcagt
     5821 tcaggctttt gctatatgct tgagcagctt cgacaccaaa ttggcatgtg aacccgggga
     5881 cccagctttc ttgtacaaag tggttgataa cagcgggtta attaacatct tttacccata
     5941 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
     6001 atatgacgtt ccagattacg ctgctcagtg cagcgcttag agctcgaatt tccccgatcg
     6061 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     6121 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     6181 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     6241 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     6301 actagatcgg gaattagctt catcaacgca agacatgcgc acgaccgtct gacaggagag
     6361 gaatttccga cgagcacaga aaggacttgc tcttggacgt aggcctattt ctcaggcaca
     6421 tgtatcaagt gttcggacgt gggttttcga tggtgtatca gccgccgcca actgggagat
     6481 gaggaggctt tcttgggggg cagtcagcag ttcatttcac aagacagagg aacttgtaag
     6541 gagatgcact gatttatctt ggcgcaaacc agcaggacga attagtggga atagcccgcg
     6601 aatatctaag ttatgcctgt cggcatgagc agaaacttcc aattcgaaac agtttggaga
     6661 ggttgttttt gggcatacct tttgttagtc agcctctcga ttgctcatcg tcattacaca
     6721 gtaccgaagt ttgatcgatc tagtaacata gatgacaccg cgcgcgataa tttatcctag
     6781 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
     6841 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
     6901 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
     6961 aaactttatt gccaaatgtt tgaacgatct gcttcgacgc actccttctt tactccacca
     7021 tctcgtcctt attgaaaacg tgggtagcac caaaacgaat caagtcgctg gaactgaagt
     7081 taccaatcac gctggatgat ttgccagttg gattaatctt gcctttcccc gcatgaataa
     7141 tattgatgaa tgcatgcgtg aggggtattt cgattttggc aatagctgca attgccgcga
     7201 catcctccaa cgagcataat tcttcagaaa aatagcgatg ttccatgttg tcagggcatg
     7261 catgatgcac gttatgaggt gacggtgcta ggcagtattc cctcaaagtt tcatagtcag
     7321 tatcatattc atcattgcat tcctgcaaga gagaattgag acgcaatcca cacgctgcgg
     7381 caaccttccg gcgttcgtgg tctatttgct cttggacgtt gcaaacgtaa gtgttggatc
     7441 ccggtcggca tctactctat tcctttgccc tcggacgagt gctggggcgt cggtttccac
     7501 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
     7561 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
     7621 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
     7681 ggagcatata cgcccggagc cgcggcgatc ctgcaagctc cggatgcctc cgctcgaagt
     7741 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
     7801 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
     7861 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
     7921 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
     7981 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
     8041 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
     8101 taagatcggc cgcagcgatc gcatccatgg cctccgcgac cggctgcaga acagcgggca
     8161 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     8221 tcaggctctc gctgaattcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     8281 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     8341 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     8401 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     8461 cggtgagttc aggctttttc atatcggggt cgtcctctcc aaatgaaatg aacttcctta
     8521 tatagaggaa gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga
     8581 gatatcacat caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat
     8641 gctcctcgtg ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat
     8701 agcctttcct ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct
     8761 tttgatgaag tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct
     8821 ttgttgaaaa gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga
     8881 gtagacgaga gtgtcgtgct ccaccatgtt gacggatctc taggacgcgt cgacgcgtcg
     8941 acgcgtcgac gcgtcgacgc gtcctagaag ctaattcact ggccgtcgtt ttacaacgtc
     9001 gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg
     9061 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
     9121 tgaatggcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     9181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     9241 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
     9301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     9361 actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg gattttgccg
     9421 atttcggaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     9481 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     9541 tgaaaagaaa aaccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     9601 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     9661 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     9721 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     9781 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     9841 tgtaacgatg acagagcgtt gctgcctgtg atcaaatatc atctccctcg cagagatccg
     9901 aattatcagc cttcttattc atttctcgct taaccgtgac aggctgtcga tcttgagaac
     9961 tatgccgaca taataggaaa tcgctggata aagccgctga ggaagctgag tggcgctatt
    10021 tctttagaag tgaacgttga cgatatcaac tcccctatcc attgctcacc gaatggtaca
    10081 ggtcggggac ccgaagttcc gactgtcggc ctgatgcatc cccggctgat cgaccccaga
    10141 tctggggctg agaaagccca gtaaggaaac aactgtaggt tcgagtcgcg agatcccccg
    10201 gaaccaaagg aagtaggtta aacccgctcc gatcaggccg agccacgcca ggccgagaac
    10261 attggttcct gtaggcatcg ggattggcgg atcaaacact aaagctactg gaacgagcag
    10321 aagtcctccg gccgccagtt gccaggcggt aaaggtgagc agaggcacgg gaggttgcca
    10381 cttgcgggtc agcacggttc cgaacgccat ggaaaccgcc cccgccaggc ccgctgcgac
    10441 gccgacagga tctagcgctg cgtttggtgt caacaccaac agcgccacgc ccgcagttcc
    10501 gcaaatagcc cccaggaccg ccatcaatcg tatcgggcta cctagcagag cggcagagat
    10561 gaacacgacc atcagcggct gcacagcgcc taccgtcgcc gcgaccccgc ccggcaggcg
    10621 gtagaccgaa ataaacaaca agctccagaa tagcgaaata ttaagtgcgc cgaggatgaa
    10681 gatgcgcatc caccagattc ccgttggaat ctgtcggacg atcatcacga gcaataaacc
    10741 cgccggcaac gcccgcagca gcataccggc gacccctcgg cctcgctgtt cgggctccac
    10801 gaaaacgccg gacagatgcg ccttgtgagc gtccttgggg ccgtcctcct gtttgaagac
    10861 cgacagccca atgatctcgc cgtcgatgta ggcgccgaat gccacggcat ctcgcaaccg
    10921 ttcagcgaac gcctccatgg gctttttctc ctcgtgctcg taaacggacc cgaacatctc
    10981 tggagctttc ttcagggccg acaatcggat ctcgcggaaa tcctgcacgt cggccgctcc
    11041 aagccgtcga atctgagcct taatcacaat tgtcaatttt aatcctctgt ttatcggcag
    11101 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
    11161 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
    11221 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
    11281 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
    11341 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
    11401 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
    11461 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
    11521 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
    11581 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
    11641 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
    11701 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
    11761 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
    11821 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
    11881 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
    11941 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
    12001 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
    12061 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
    12121 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
    12181 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
    12241 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
    12301 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    12361 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    12421 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    12481 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    12541 atcttgccct gcacgaatac cagcgacccc ttgcccaaat acttgccgtg ggcctcggcc
    12601 tgagagccaa aacacttgat gcggaagaag tcggtgcgct cctgcttgtc gccggcatcg
    12661 ttgcgccaca tctaggtact aaaacaattc atccagtaaa atataatatt ttattttctc
    12721 ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt cttccccgat
    12781 atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg ccctgccgct
    12841 tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt tgctgtctcc
    12901 caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta aaaaatcata
    12961 cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat ccacatcggc
    13021 cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc tattcgtata
    13081 gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat acagctcgat
    13141 aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc catcggcctc
    13201 actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct ttggaacagg
    13261 cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat aggtggtccc
    13321 tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca ccagcttata
    13381 taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt cgatcagttt
    13441 tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc ttttctacag
    13501 tatttaaaga taccccaaga agctaattat aacaagacga actccaattc actgttcctt
    13561 gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc aaagttggcg
    13621 tataacatag tatcgacgga gccgattttg aaaccacaat tatgggtgat gctgccaact
    13681 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
    13741 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
    13801 cagcgctatc tctgctctca ctgccgtaaa acatggcaac tgcagttcac ttacaccgct
    13861 tctcaacccg gtacgcacca gaaaatcatt gatatggcca tgaatggcgt tggatgccgg
    13921 gcaacagccc gcattatggg cgttggcctc aacacgattt tacgtcactt aaaaaactca
    13981 ggccgcagtc ggtaacctcg cgcatacagc cgggcagtga cgtcatcgtc tgcgcggaaa
    14041 tggacgaaca gtggggctat gtcggggcta aatcgcgcca gcgctggctg ttttacgcgt
    14101 atgacagtct ccggaagacg gttgttgcgc acgtattcgg tgaacgcact atggcgacgc
    14161 tggggcgtct tatgagcctg ctgtcaccct ttgacgtggt gatatggatg acggatggct
    14221 ggccgctgta tgaatcccgc ctgaagggaa agctgcacgt aatcagcaag cgatatacgc
    14281 agcgaattga gcggcataac ctgaatctga ggcagcacct ggcacggctg ggacggaagt
    14341 cgctgtcgtt ctcaaaatcg gtggagctgc atgacaaagt catcgggcat tatctgaaca
    14401 taaaacacta tcaataagtt ggagtcatta cccaattatg atagaattta caagctataa
    14461 ggttattgtc ctgggtttca agcattagtc catgcaagtt tttatgcttt gcccattcta
    14521 tagatatatt gataagcgcg ctgcctatgc cttgccccct gaaatcctta catacggcga
    14581 tatcttctat ataaaagata tattatctta tcagtattgt caatatattc aaggcaatct
    14641 gcctcctcat cctcttcatc ctcttcgtct tggtagcttt ttaaatatgg cgcttcatag
    14701 agtaattctg taaaggtcca attctcgttt tcatacctcg gtataatctt acctatcacc
    14761 tcaaatggtt cgctgggttt atcgcacccc cgaacacgag cacggcaccc gcgaccacta
    14821 tgccaagaat gcccaaggta aaaattgccg gccccgccat gaagtccgtg aatgccccga
    14881 cggccgaagt gaagggcagg ccgccaccca ggccgccgcc ctcactgccc ggcacctggt
    14941 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    15001 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    15061 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    15121 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    15181 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    15241 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    15301 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    15361 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    15421 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    15481 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    15541 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    15601 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    15661 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    15721 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgc
//
