LOCUS       sequence-247568-       15902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15902)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX161
FEATURES             Location/Qualifiers
     source          1..15902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6048..6072)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6101..6190
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6224..6476
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6905..7157)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7625..8650)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7625..8650)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8660..9004)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9114..9131)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9114..9130)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9123..9145)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9270..9289)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9480..9501
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9748..9772
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11368..12516)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12815..13609)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13801..14568
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      15261..15892
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggggaa
     4621 gggttcgaag agcggtgcgg gcaagtctgc atcgcaccag ctcttcaagg acaaggcaaa
     4681 gaaccgcgtg gatgatctgc agggcgtgtt cactaacctg caatctgcaa ggaaggagag
     4741 tcgaacttat gatgttgggg tgcttgaaga gcaggttcac cagatgcttc gtgagtggaa
     4801 ggctgagctc aatgagcctt ccccggcttc ttcattgcag ggaggaagcc gtgtctcatc
     4861 agatatctat agactgctgc ttggtgagga ggaagatgac gctaccagcg cattagctgc
     4921 acctaagcct gagcctgatg ctcaaaaaaa tgatgttgct gcatttcaag agggtttcaa
     4981 tgtaactcca gtgctgcagg agcaaggtta ccagttggtt gatcagtgca aaagtatgcc
     5041 tttagtggtc aacaatgcag ggattaacaa cctaggcatt gcaacacaac aagattacaa
     5101 ttcttttgat ctgcagcaag actttgatca gtacttcccc ggatttgatg ctttaaatct
     5161 ttgtctagag gatgtgttgc ctcctgttca tatcttccct ccaccctctg cgttcctggg
     5221 tcctaaatgt tcactttggg attgtccccg acctgcgatg gggtcagatt ggtgtcagaa
     5281 gtctcttgac tactgcagcg actatcatgc ttctcttgcg cctaatgaag gctatcctgg
     5341 aacacctcca cttgttagac cgatgggtat tggcttaaag gataatttgc ttttccaagc
     5401 gctaagtgca aaagcacagg ggaaagatgt tggcattcct gagtgtgaag gtgctgccac
     5461 tgcaaaatct ccttggaatg caccagagct ctttgacctt aaagttgttg aaggcgaaac
     5521 aatcagggaa tggattttct ttgataagcc tcgaagagca tttgaaagcg gaaacagaaa
     5581 gcagaggtca ctgccagatt ataacggacg gggttggcat gagtctagga aacaagtgat
     5641 gaatgaatac ggagggctga agagatccta ctatatggat ccacagccga tgaaaaatct
     5701 ggaatggcat ctgtatgaat atgagatcaa caagtatgat gcgtgtgccc tgtacagatt
     5761 ggagctgaaa cttgttgatg agaagaagag tccaaaaggc aaagtaacga aggaatcagt
     5821 tgctgatttg caaaagcaaa tgggaagact cactgctgaa tttcagtcgg agaacaagcg
     5881 ctctgttaaa ggtagagcaa aagctaactc aaaggatgtt gctgttaaca tgcatgctgt
     5941 tccaaatcta attgttccag ctagtgaagg gcttgattat gggaccggtg caccttttcc
     6001 tgactatctc gttgataatt taggtggcta ctacgtaact cccggggacc cagctttctt
     6061 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     6121 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     6181 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     6241 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     6301 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6361 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6421 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     6481 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     6541 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     6601 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     6661 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     6721 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     6781 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     6841 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     6901 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6961 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     7021 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     7081 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     7141 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     7201 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     7261 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     7321 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     7381 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     7441 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     7501 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     7561 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     7621 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     7681 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     7741 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     7801 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     7861 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     7921 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     7981 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     8041 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     8101 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     8161 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     8221 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     8281 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     8341 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     8401 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     8461 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     8521 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     8581 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     8641 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     8701 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     8761 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     8821 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     8881 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     8941 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     9001 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     9061 gtcgtgctcc accatgttga cggatctcta ggacgcgtcc tagaagctaa ttcactggcc
     9121 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9181 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9241 caacagttgc gcagcctgaa tggcgcccgc tcctttcgct ttcttccctt cctttctcgc
     9301 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     9361 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
     9421 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     9481 tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt
     9541 ataagggatt ttgccgattt cggaaccacc atcaaacagg attttcgcct gctggggcaa
     9601 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     9661 ttgcccgtct cactggtgaa aagaaaaacc accccagtac attaaaaacg tccgcaatgt
     9721 gttattaagt tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag
     9781 ccaacagctc cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc
     9841 agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc
     9901 gatgctattc ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg
     9961 agggtagcat gttgattgta acgatgacag agcgttgctg cctgtgatca aatatcatct
    10021 ccctcgcaga gatccgaatt atcagccttc ttattcattt ctcgcttaac cgtgacaggc
    10081 tgtcgatctt gagaactatg ccgacataat aggaaatcgc tggataaagc cgctgaggaa
    10141 gctgagtggc gctatttctt tagaagtgaa cgttgacgat atcaactccc ctatccattg
    10201 ctcaccgaat ggtacaggtc ggggacccga agttccgact gtcggcctga tgcatccccg
    10261 gctgatcgac cccagatctg gggctgagaa agcccagtaa ggaaacaact gtaggttcga
    10321 gtcgcgagat cccccggaac caaaggaagt aggttaaacc cgctccgatc aggccgagcc
    10381 acgccaggcc gagaacattg gttcctgtag gcatcgggat tggcggatca aacactaaag
    10441 ctactggaac gagcagaagt cctccggccg ccagttgcca ggcggtaaag gtgagcagag
    10501 gcacgggagg ttgccacttg cgggtcagca cggttccgaa cgccatggaa accgcccccg
    10561 ccaggcccgc tgcgacgccg acaggatcta gcgctgcgtt tggtgtcaac accaacagcg
    10621 ccacgcccgc agttccgcaa atagccccca ggaccgccat caatcgtatc gggctaccta
    10681 gcagagcggc agagatgaac acgaccatca gcggctgcac agcgcctacc gtcgccgcga
    10741 ccccgcccgg caggcggtag accgaaataa acaacaagct ccagaatagc gaaatattaa
    10801 gtgcgccgag gatgaagatg cgcatccacc agattcccgt tggaatctgt cggacgatca
    10861 tcacgagcaa taaacccgcc ggcaacgccc gcagcagcat accggcgacc cctcggcctc
    10921 gctgttcggg ctccacgaaa acgccggaca gatgcgcctt gtgagcgtcc ttggggccgt
    10981 cctcctgttt gaagaccgac agcccaatga tctcgccgtc gatgtaggcg ccgaatgcca
    11041 cggcatctcg caaccgttca gcgaacgcct ccatgggctt tttctcctcg tgctcgtaaa
    11101 cggacccgaa catctctgga gctttcttca gggccgacaa tcggatctcg cggaaatcct
    11161 gcacgtcggc cgctccaagc cgtcgaatct gagccttaat cacaattgtc aattttaatc
    11221 ctctgtttat cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca
    11281 agtgcgtcga gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc
    11341 cccagccgga actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac
    11401 ccaggcgtgt tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc
    11461 gcgccacttc ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt
    11521 gagcgggtac ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga
    11581 cagcttgcgg tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac
    11641 gatttcctcg tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg
    11701 gaagcggtgc agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat
    11761 cgccgtcgcc tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat
    11821 cgaccagccc aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg
    11881 ggtgcgcttc gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag
    11941 ctcgacgccg gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg
    12001 cagcgcctcg cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc
    12061 gtttggcatc gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat
    12121 ttccttgatc tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg
    12181 ttttgccagg tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc
    12241 gatggtcatc gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac
    12301 ggcggccgat ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt
    12361 ggccgtagct tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat
    12421 gacggtgcgg cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc
    12481 ttgcctgtat gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc
    12541 gactcacgcc ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc
    12601 cagataatcc accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc
    12661 gtacttggta ttccgaatct tgccctgcac gaataccagc gaccccttgc ccaaatactt
    12721 gccgtgggcc tcggcctgag agccaaaaca cttgatgcgg aagaagtcgg tgcgctcctg
    12781 cttgtcgccg gcatcgttgc gccacatcta ggtactaaaa caattcatcc agtaaaatat
    12841 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
    12901 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
    12961 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
    13021 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
    13081 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
    13141 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
    13201 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
    13261 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
    13321 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
    13381 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
    13441 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
    13501 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
    13561 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
    13621 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
    13681 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
    13741 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cacaattatg
    13801 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
    13861 ttctgtttct atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa
    13921 aagcaccgcc ggacatcagc gctatctctg ctctcactgc cgtaaaacat ggcaactgca
    13981 gttcacttac accgcttctc aacccggtac gcaccagaaa atcattgata tggccatgaa
    14041 tggcgttgga tgccgggcaa cagcccgcat tatgggcgtt ggcctcaaca cgattttacg
    14101 tcacttaaaa aactcaggcc gcagtcggta acctcgcgca tacagccggg cagtgacgtc
    14161 atcgtctgcg cggaaatgga cgaacagtgg ggctatgtcg gggctaaatc gcgccagcgc
    14221 tggctgtttt acgcgtatga cagtctccgg aagacggttg ttgcgcacgt attcggtgaa
    14281 cgcactatgg cgacgctggg gcgtcttatg agcctgctgt caccctttga cgtggtgata
    14341 tggatgacgg atggctggcc gctgtatgaa tcccgcctga agggaaagct gcacgtaatc
    14401 agcaagcgat atacgcagcg aattgagcgg cataacctga atctgaggca gcacctggca
    14461 cggctgggac ggaagtcgct gtcgttctca aaatcggtgg agctgcatga caaagtcatc
    14521 gggcattatc tgaacataaa acactatcaa taagttggag tcattaccca attatgatag
    14581 aatttacaag ctataaggtt attgtcctgg gtttcaagca ttagtccatg caagttttta
    14641 tgctttgccc attctataga tatattgata agcgcgctgc ctatgccttg ccccctgaaa
    14701 tccttacata cggcgatatc ttctatataa aagatatatt atcttatcag tattgtcaat
    14761 atattcaagg caatctgcct cctcatcctc ttcatcctct tcgtcttggt agctttttaa
    14821 atatggcgct tcatagagta attctgtaaa ggtccaattc tcgttttcat acctcggtat
    14881 aatcttacct atcacctcaa atggttcgct gggtttatcg cacccccgaa cacgagcacg
    14941 gcacccgcga ccactatgcc aagaatgccc aaggtaaaaa ttgccggccc cgccatgaag
    15001 tccgtgaatg ccccgacggc cgaagtgaag ggcaggccgc cacccaggcc gccgccctca
    15061 ctgcccggca cctggtcgct gaatgtcgat gccagcacct gcggcacgtc aatgcttccg
    15121 ggcgtcgcgc tcgggctgat cgcccatccc gttactgccc cgatcccggc aatggcaagg
    15181 actgccagcg ctgccatttt tggggtgagg ccgttcgcgg ccgaggggcg cagcccctgg
    15241 ggggatggga ggcccgcgtt agcgggccgg gagggttcga gaaggggggg cacccccctt
    15301 cggcgtgcgc ggtcacgcgc acagggcgca gccctggtta aaaacaaggt ttataaatat
    15361 tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc ggaaaccctt
    15421 gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt gcgcccctca
    15481 tctgtcagca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt cagtagtcgc
    15541 gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca tcatctgtgg
    15601 gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc tccacgtcgc
    15661 cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt cggcccctca
    15721 agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcga ggtatccaca
    15781 acgccggcgg ccgcggtgtc tcgcacacgg cttcgacggc gtttctggcg cgtttgcagg
    15841 gccatagacg gccgccagcc cagcggcgag ggcaaccagc ccggtgagcg tcggaaaggc
    15901 gc
//
