LOCUS       sequence-247571-       17244 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17244)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 17244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX164
FEATURES             Location/Qualifiers
     source          1..17244
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7358..7382)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7411..7500
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7534..7786
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(8215..8467)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8935..9960)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8935..9960)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9970..10314)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10456..10473)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10456..10472)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10465..10487)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10612..10631)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10822..10843
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    11090..11114
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12710..13858)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(14157..14951)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  15143..15910
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16603..17234
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggcttcccat gctggaaact tcagttttgt tggagaattc agctactgct
     5881 gttgctggtg gcgccgccgt gactcaggtg gtggagctga ggaatgacgg cgtcggcggt
     5941 ggtggtagtg tcggtggagg ttcagaagag gaagagagaa gcagaggaga attagaaggt
     6001 gaaaagaata atatatcagg tggaaatcgt tggccacgtg atgaaacttt ggctttgctc
     6061 aaaataaggt ctcaaatgga ccttgcattc cgtgattcca atctcaaagg ccctctttgg
     6121 gatgaaattt ctaggaaaat gggtgagcta gggtataatc gaaatgcaaa gaaatgcagg
     6181 gagaaatttg agaacattta taagtatcat aagagaacaa aagatggtag atctggcagg
     6241 caaacgggga aaaactatcg gtttttcgag caattagaac tattggataa ccagattaat
     6301 aggatggata caacaacatc aatttcaatg ccagtaccaa tgcctatgcc gatgacaatg
     6361 ataaaaccag ccacaagtgg atgtcaaaat ttcacttatc gaaaccaagg tgttaatccg
     6421 gagttcatgt ctatgtccac atctacaacc tcttcctcgg gaaaggaatc cgatggtagt
     6481 gtgaagaaga aaaggaaatt ggctggttat ttcgagaggt tgatgaagca agtgttggac
     6541 aagcaagagg atttacaaaa caagttcttg gaagcaatag agaaatgcga gggggatcga
     6601 atagagaggg aagaagcttg gaagatgcag gaaatagcaa ggttaaagaa agaaaaagaa
     6661 gcactagcca atgaaagagc aatttctgct gccaaagatg cagcagtgat tgctttcttg
     6721 cagaaaatct cagagcaaac cgttcaagta cagtcgccaa tggaattatc tcatgaaaag
     6781 atgactgaga attcgtcggt gaaaacggtt gaaagccaag agaatgtgtt gcagcaagac
     6841 gatgataagc aagagaacat gttggacaaa caagatataa tagatagtgc tggtgagaat
     6901 tcgtttcata tgagttcttg tcgatggcct aaatcagaag tcgaagcatt gatcaagtta
     6961 agaacgaaca ttgacttgga atatcaagac aatggatcac caaaagggcc tctatgggaa
     7021 gatatctcaa ctggtatgaa gaagcttgga tatgatagaa atgccaagag gtgtaaagag
     7081 aaatgggaga acataaacaa gtattatagg agagttaaag aaagccaaaa gaggaggcca
     7141 gaagattcaa aaacatgtcc ctattttcat ctactggatt ctatctatca aaacaagtcc
     7201 aagaaacaat tgctcagttc agaaaatcca ggttctagta taaaggctgg agagttacta
     7261 atgcaaataa tgaaccgaca acagcagcag caacagttgg aaacagaaaa tattcaaaga
     7321 caaaatgttg aacgaagtca atcgaggacc cagcttgacc cagctttctt gtacaaagtg
     7381 gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga ctatgcgggc
     7441 tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc agattacgct
     7501 gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     7561 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     7621 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     7681 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     7741 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attagcttca
     7801 tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg agcacagaaa
     7861 ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt tcggacgtgg
     7921 gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc ttggggggca
     7981 gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga tttatcttgg
     8041 cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt atgcctgtcg
     8101 gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg gcataccttt
     8161 tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt gatcgatcta
     8221 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
     8281 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
     8341 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
     8401 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
     8461 aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat tgaaaacgtg
     8521 ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc tggatgattt
     8581 gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg catgcgtgag
     8641 gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg agcataattc
     8701 ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt tatgaggtga
     8761 cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat cattgcattc
     8821 ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc gttcgtggtc
     8881 tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc tactctattc
     8941 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
     9001 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
     9061 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
     9121 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg
     9181 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
     9241 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
     9301 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
     9361 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
     9421 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
     9481 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
     9541 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
     9601 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg
     9661 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc
     9721 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
     9781 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
     9841 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
     9901 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
     9961 atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
    10021 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
    10081 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
    10141 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
    10201 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
    10261 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
    10321 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
    10381 accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc gtcgacgcgt
    10441 cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
    10501 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
    10561 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc gctcctttcg
    10621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    10681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    10741 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    10801 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    10861 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca ccatcaaaca
    10921 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
    10981 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccccagt
    11041 acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac
    11101 aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac
    11161 cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc
    11221 ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt
    11281 tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc
    11341 tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct tcttattcat
    11401 ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata ataggaaatc
    11461 gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg aacgttgacg
    11521 atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc gaagttccga
    11581 ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag aaagcccagt
    11641 aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa gtaggttaaa
    11701 cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt aggcatcggg
    11761 attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc cgccagttgc
    11821 caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag cacggttccg
    11881 aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc tagcgctgcg
    11941 tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc caggaccgcc
    12001 atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat cagcggctgc
    12061 acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat aaacaacaag
    12121 ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca ccagattccc
    12181 gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc ccgcagcagc
    12241 ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga cagatgcgcc
    12301 ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat gatctcgccg
    12361 tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc ctccatgggc
    12421 tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt cagggccgac
    12481 aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat ctgagcctta
    12541 atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc gccgtgcgtc
    12601 ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc tgaaatgcca
    12661 gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc
    12721 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt
    12781 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca
    12841 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga
    12901 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt
    12961 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt
    13021 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa
    13081 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct
    13141 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga
    13201 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca
    13261 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga
    13321 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga
    13381 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa
    13441 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg
    13501 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg
    13561 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt
    13621 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt
    13681 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga
    13741 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg
    13801 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc
    13861 accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat tcctgatttg
    13921 acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag tcggtcccgt
    13981 agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc acgaatacca
    14041 gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa cacttgatgc
    14101 ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc taggtactaa
    14161 aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt gatccccagt
    14221 aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat cgaccggacg
    14281 cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc aataaagcca
    14341 cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg ggaaaagaca
    14401 agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg atctttaaat
    14461 ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt cagtaagtaa
    14521 tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga tatgtcgatg
    14581 gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg gctttgttca
    14641 tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag attgctccag
    14701 ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc cagccatagc
    14761 atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct gtccgtcatt
    14821 tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg agacattcct
    14881 tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg tgatattctc
    14941 attttagcca tttattattt ccttcctctt ttctacagta tttaaagata ccccaagaag
    15001 ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac cttaaatacc
    15061 agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta tcgacggagc
    15121 cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt gtatgatggt
    15181 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
    15241 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc tgctctcact
    15301 gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt acgcaccaga
    15361 aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc attatgggcg
    15421 ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg taacctcgcg
    15481 catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt ggggctatgt
    15541 cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc ggaagacggt
    15601 tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta tgagcctgct
    15661 gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg aatcccgcct
    15721 gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc ggcataacct
    15781 gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct caaaatcggt
    15841 ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc aataagttgg
    15901 agtcattacc caattatgat agaatttaca agctataagg ttattgtcct gggtttcaag
    15961 cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga taagcgcgct
    16021 gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat aaaagatata
    16081 ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc tcttcatcct
    16141 cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta aaggtccaat
    16201 tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg ctgggtttat
    16261 cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc ccaaggtaaa
    16321 aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga agggcaggcc
    16381 gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg atgccagcac
    16441 ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc ccgttactgc
    16501 cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga ggccgttcgc
    16561 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
    16621 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt
    16681 taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt tagcggtggc
    16741 cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac agcccctcaa
    16801 atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc aaggatcgcg
    16861 cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca cttatcccca
    16921 ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg ccgatttgcg
    16981 aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct gtcaacgccg
    17041 cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca gtgagggcca
    17101 agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac ggcttcgacg
    17161 gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg agggcaacca
    17221 gcccggtgag cgtcggaaag gcgc
//
