LOCUS       sequence-247573-       15388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15388)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX165
FEATURES             Location/Qualifiers
     source          1..15388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5502..5526)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5555..5644
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5678..5930
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6359..6611)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7079..8104)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7079..8104)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8114..8458)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8600..8617)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8600..8616)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8609..8631)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8756..8775)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8966..8987
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9234..9258
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10854..12002)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12301..13095)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13287..14054
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14747..15378
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggatca
     4621 tttaatccgg ttcgtttcac ttcagcagca gaaaaaccaa ccggttcagc cggattgtag
     4681 agaactcgcc gacttcactg tttctaattt taagaaggtt atttctatct tgaaccggac
     4741 cggtcatgcc cggtttagac gcagtccggt tcagctttct gattcatgta ctgctctttc
     4801 cctatctacg tctactacgg ttaaggaaac tccaccaccg ccgacacagt cgactcctcc
     4861 aactccggtg gttgctctgg tggaacacac tcaatatccg gcattgaaat ttgactttac
     4921 aaaatcaaag gttgcgaagg aaactttgag cttatcgtcg aactcatcgt cgttcatgtc
     4981 gtcgattacc ggggattgtg aaggaagtgt atcaaacggg aagcaatttt cctcaattag
     5041 cttaccgccg cggccgcctg tgtcatccgg gaagccgcca cttgccggaa aaagatgtcg
     5101 cgatcacgac ctttccgacg aaatgtccgg tagaatctcc ggctccggca attgtcactg
     5161 caaaaagagg aaatatcgtg caaagaaagt tattagagtg ccggcggtca gttcaaaaat
     5221 tgccgatatt ccagcagaca agtattcctg gagaaaatac ggtcaaaagc cgatcaaggg
     5281 ctcaccatac ccacggggat attatagatg tagcagctta agaggatgtc cggcaagaaa
     5341 gcatgtggag agggcaaccg atgatcccaa aatggttatt gtaacttacg aaggagagca
     5401 tcgtcatgta attgaaggga cgatcgccgg cgcaggagct gtaagttccg acgagagaat
     5461 gatggtgttc gattcaacgg agcagagaaa aggacccggg gacccagctt tcttgtacaa
     5521 agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc ctgactatgc
     5581 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
     5641 cgctgctcag tgcagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     5701 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     5761 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     5821 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     5881 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattagc
     5941 ttcatcaacg caagacatgc gcacgaccgt ctgacaggag aggaatttcc gacgagcaca
     6001 gaaaggactt gctcttggac gtaggcctat ttctcaggca catgtatcaa gtgttcggac
     6061 gtgggttttc gatggtgtat cagccgccgc caactgggag atgaggaggc tttcttgggg
     6121 ggcagtcagc agttcatttc acaagacaga ggaacttgta aggagatgca ctgatttatc
     6181 ttggcgcaaa ccagcaggac gaattagtgg gaatagcccg cgaatatcta agttatgcct
     6241 gtcggcatga gcagaaactt ccaattcgaa acagtttgga gaggttgttt ttgggcatac
     6301 cttttgttag tcagcctctc gattgctcat cgtcattaca cagtaccgaa gtttgatcga
     6361 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     6421 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     6481 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     6541 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     6601 tttgaacgat ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa
     6661 cgtgggtagc accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg
     6721 atttgccagt tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg
     6781 tgaggggtat ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata
     6841 attcttcaga aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag
     6901 gtgacggtgc taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc
     6961 attcctgcaa gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt
     7021 ggtctatttg ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg catctactct
     7081 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     7141 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     7201 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     7261 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     7321 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     7381 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     7441 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     7501 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     7561 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     7621 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     7681 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     7741 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     7801 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     7861 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     7921 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     7981 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     8041 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     8101 tcatatcggg gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     8161 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     8221 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     8281 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     8341 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     8401 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     8461 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     8521 ctccaccatg ttgacggatc tctaggacgc gtcgacgcgt cgacgcgtcg acgcgtcgac
     8581 gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     8641 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     8701 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct
     8761 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     8821 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     8881 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     8941 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9001 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca
     9061 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     9121 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     9181 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
     9241 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
     9301 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
     9361 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
     9421 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
     9481 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
     9541 tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga
     9601 aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt
     9661 gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt
     9721 ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc
     9781 cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt
     9841 taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat
     9901 cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag
     9961 ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt
    10021 tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc
    10081 tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac
    10141 cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg
    10201 ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa
    10261 caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat
    10321 tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag
    10381 cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg
    10441 cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc
    10501 gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat
    10561 gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc
    10621 cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc
    10681 cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg
    10741 cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat
    10801 gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt
    10861 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
    10921 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
    10981 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
    11041 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
    11101 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
    11161 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
    11221 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
    11281 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
    11341 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
    11401 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
    11461 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
    11521 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
    11581 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
    11641 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
    11701 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
    11761 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
    11821 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac
    11881 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
    11941 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
    12001 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
    12061 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
    12121 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
    12181 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
    12241 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta
    12301 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    12361 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    12421 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    12481 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    12541 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    12601 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    12661 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    12721 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    12781 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    12841 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    12901 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    12961 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    13021 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    13081 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    13141 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    13201 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    13261 gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga
    13321 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
    13381 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct
    13441 cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac
    13501 cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg
    13561 ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct
    13621 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct
    13681 atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga
    13741 cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc
    13801 tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
    13861 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata
    13921 acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat
    13981 cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag
    14041 ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt
    14101 caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg
    14161 cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga
    14221 tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca
    14281 tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc
    14341 caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt
    14401 ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg
    14461 taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca
    14521 ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca
    14581 gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta
    14641 ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt
    14701 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
    14761 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
    14821 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
    14881 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
    14941 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
    15001 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
    15061 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
    15121 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
    15181 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
    15241 gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc
    15301 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
    15361 accagcccgg tgagcgtcgg aaaggcgc
//
