LOCUS       sequence-247574-       15499 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15499)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15499)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX166
FEATURES             Location/Qualifiers
     source          1..15499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5613..5637)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5666..5755
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5789..6041
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6470..6722)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7190..8215)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7190..8215)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8225..8569)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8711..8728)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8711..8727)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8720..8742)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8867..8886)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9077..9098
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9345..9369
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10965..12113)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12412..13206)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13398..14165
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14858..15489
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggcagt
     4621 tgattttgtt gggttttcaa aaatgaatga gcaattagct cttcaagaag ctgcttcagc
     4681 cggtttaaaa tctatggaac atttaatccg gttggtttct caccaacaac agcagcagcc
     4741 ggttcagctc gattgccgcg agataactga ctttaccgtc tcgaaattta ggaaggttat
     4801 ttctattctg gaccggaccg gtcatgctcg gttccgccgt ggtcaggttc aggttcatcc
     4861 tgataatttt acttctctgt ccctttctcc gtcaaatcag cagctgttaa acttagctcc
     4921 ggcgaaagag acaccgccgc ctcggccacc atcagtgtcg ccgccattaa cggcgttgac
     4981 gcttgacttt acgaagccaa acgttgaccg tccggcggga aattctaatg ctattgttgc
     5041 tgtgaagtca aaggagactt tctgtatatc tacgccgatg gcgacttcga cgaactcgtc
     5101 gtcgttcatt tcgtcgatta ccggcgaagg aagtgtatca aacggaaaac aaggttcgtc
     5161 aatgtttttg cctccggcac aagctgtttc cgccgggaaa ccaccagtag ccggtaaaag
     5221 atgccgcgag cacgaatact ctgaagatat ctctggcaaa tccaccggct ccggcagatg
     5281 tcactgcaaa aagaggaaat ctcgtgtaaa gaaagttgta agaatcccag cgataagttc
     5341 gagaattgcc gatataccgg gagatgagtt ctcgtggaga aaatacgggc agaagccgat
     5401 caagggttca ccatacccac ggggatatta taagtgtagc agcgtaagag gatgtccagc
     5461 gaagaaacac gtggaaaggg caattgatga tcctgctatg ctgattgtga cttatgaagg
     5521 ggaacatcgt catacgatag gcgcaataca ggagaacaat tctcaaatga tggcgtttgg
     5581 gtcaacggaa gaggaaggag tgaaacccgg ggacccagct ttcttgtaca aagtggttga
     5641 taacagcggg ttaattaaca tcttttaccc atacgatgtt cctgactatg cgggctatcc
     5701 ctatgacgtc ccggactatg caggatccta tccatatgac gttccagatt acgctgctca
     5761 gtgcagcgct tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt
     5821 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     5881 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     5941 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6001 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattag cttcatcaac
     6061 gcaagacatg cgcacgaccg tctgacagga gaggaatttc cgacgagcac agaaaggact
     6121 tgctcttgga cgtaggccta tttctcaggc acatgtatca agtgttcgga cgtgggtttt
     6181 cgatggtgta tcagccgccg ccaactggga gatgaggagg ctttcttggg gggcagtcag
     6241 cagttcattt cacaagacag aggaacttgt aaggagatgc actgatttat cttggcgcaa
     6301 accagcagga cgaattagtg ggaatagccc gcgaatatct aagttatgcc tgtcggcatg
     6361 agcagaaact tccaattcga aacagtttgg agaggttgtt tttgggcata ccttttgtta
     6421 gtcagcctct cgattgctca tcgtcattac acagtaccga agtttgatcg atctagtaac
     6481 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     6541 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     6601 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     6661 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     6721 tctgcttcga cgcactcctt ctttactcca ccatctcgtc cttattgaaa acgtgggtag
     6781 caccaaaacg aatcaagtcg ctggaactga agttaccaat cacgctggat gatttgccag
     6841 ttggattaat cttgcctttc cccgcatgaa taatattgat gaatgcatgc gtgaggggta
     6901 tttcgatttt ggcaatagct gcaattgccg cgacatcctc caacgagcat aattcttcag
     6961 aaaaatagcg atgttccatg ttgtcagggc atgcatgatg cacgttatga ggtgacggtg
     7021 ctaggcagta ttccctcaaa gtttcatagt cagtatcata ttcatcattg cattcctgca
     7081 agagagaatt gagacgcaat ccacacgctg cggcaacctt ccggcgttcg tggtctattt
     7141 gctcttggac gttgcaaacg taagtgttgg atcccggtcg gcatctactc tattcctttg
     7201 ccctcggacg agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat
     7261 cggtccagac ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg
     7321 atcggacgat tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa
     7381 ccaagctctg atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg
     7441 atcctgcaag ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca
     7501 accacggcct ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct
     7561 cgctccagtc aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga
     7621 aatccgcgtg cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat
     7681 cgagagcctg cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca
     7741 catggggatc agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt
     7801 gcggtccgaa tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca
     7861 tggcctccgc gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg
     7921 tgacaccctg tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat tccccaatgt
     7981 caagcacttc cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt
     8041 tgtagaaacc atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga
     8101 agctgaaagc acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga
     8161 acttttcgat cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatcgg
     8221 ggtcgtcctc tccaaatgaa atgaacttcc ttatatagag gaagggtctt gcgaaggata
     8281 gtgggattgt gcgtcatccc ttacgtcagt ggagatatca catcaatcca cttgctttga
     8341 agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt
     8401 gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca
     8461 tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca
     8521 atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg
     8581 gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat
     8641 gttgacggat ctctaggacg cgtcgacgcg tcgacgcgtc gacgcgtcga cgcgtcctag
     8701 aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
     8761 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
     8821 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc tttcgctttc
     8881 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     8941 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt
     9001 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     9061 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     9121 ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc aaacaggatt
     9181 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg
     9241 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ccagtacatt
     9301 aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac accacaatat
     9361 atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa atcaccactc
     9421 gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt aaggcggcag
     9481 actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc gggtttgaaa
     9541 cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc gttgctgcct
     9601 gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta ttcatttctc
     9661 gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg aaatcgctgg
     9721 ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt tgacgatatc
     9781 aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt tccgactgtc
     9841 ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc ccagtaagga
     9901 aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg ttaaacccgc
     9961 tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca tcgggattgg
    10021 cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca gttgccaggc
    10081 ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg ttccgaacgc
    10141 catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg ctgcgtttgg
    10201 tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga ccgccatcaa
    10261 tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg gctgcacagc
    10321 gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca acaagctcca
    10381 gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga ttcccgttgg
    10441 aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca gcagcatacc
    10501 ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat gcgccttgtg
    10561 agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct cgccgtcgat
    10621 gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca tgggcttttt
    10681 ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg ccgacaatcg
    10741 gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag ccttaatcac
    10801 aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt gcgtcccgag
    10861 cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa tgccagtaaa
    10921 gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc
    10981 accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag
    11041 gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg
    11101 cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc
    11161 cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg
    11221 ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg
    11281 ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg
    11341 tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg
    11401 taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg
    11461 atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg
    11521 tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg
    11581 aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg
    11641 gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg
    11701 aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc
    11761 ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc
    11821 atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttcgaga
    11881 cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg
    11941 ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga ctggaaggtt
    12001 tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac
    12061 cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct
    12121 ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg atttgacccg
    12181 cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt cccgtagacc
    12241 gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa taccagcgac
    12301 cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt gatgcggaag
    12361 aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt actaaaacaa
    12421 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    12481 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    12541 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    12601 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    12661 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    12721 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    12781 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    12841 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    12901 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    12961 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    13021 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    13081 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    13141 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    13201 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    13261 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    13321 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    13381 ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg atggtgtttt
    13441 tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc tactgacggg
    13501 gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc tcactgccgt
    13561 aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca ccagaaaatc
    13621 attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat gggcgttggc
    13681 ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
    13741 agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc tatgtcgggg
    13801 ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag acggttgttg
    13861 cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc ctgctgtcac
    13921 cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc cgcctgaagg
    13981 gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat aacctgaatc
    14041 tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa tcggtggagc
    14101 tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa gttggagtca
    14161 ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt tcaagcatta
    14221 gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc gcgctgccta
    14281 tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag atatattatc
    14341 ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc atcctcttcg
    14401 tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt ccaattctcg
    14461 ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg tttatcgcac
    14521 ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag gtaaaaattg
    14581 ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc aggccgccac
    14641 ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc agcacctgcg
    14701 gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt actgccccga
    14761 tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg
    14821 aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag ggttcgagaa
    14881 gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc ctggttaaaa
    14941 acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg gtggccgaaa
    15001 aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc ctcaaatgtc
    15061 aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga tcgcgcccct
    15121 catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat ccccaggctt
    15181 gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat ttgcgaggct
    15241 ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa cgccgcgccg
    15301 ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag ggccaagttt
    15361 tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt cgacggcgtt
    15421 tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc aaccagcccg
    15481 gtgagcgtcg gaaaggcgc
//
