LOCUS       sequence-247575-       15520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15520)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX167
FEATURES             Location/Qualifiers
     source          1..15520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5634..5658)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5687..5776
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5810..6062
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6491..6743)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7211..8236)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7211..8236)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8246..8590)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8732..8749)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8732..8748)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8741..8763)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8888..8907)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9098..9119
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9366..9390
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10986..12134)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12433..13227)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13419..14186
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14879..15510
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggaatt
     4621 cacaagttta gttgatactt ccttggatct gaattttaga cctcttcgag ttcctgatgg
     4681 aataccgaaa caagaagttg agagtaattt tattgggctt ggaatagatc acacgccagt
     4741 caaggatgag gcaagtgatt tgttagagga actaaataga gtaagtgctg aaaacaagaa
     4801 gctaacggag atgttaacgg taatgtgcca gaattacaat gcattgagaa accaagtgac
     4861 ggagtatatg agcaagcaca atactactac tactacttca gcagatgatc atagcgctgg
     4921 atcaaagaaa agaaaatctg aaaacaatga aatattgaaa tcagttcaag gagagagtag
     4981 ctcaagtgat gaagacgact cttgtacaaa gaaaccaaga gaagagcaca ttaaaactaa
     5041 gacaactaga gtttatgtca gaactgaacc atctgatact tctcttattg tgaaggatgg
     5101 ataccaatgg aggaaatatg gtcagaaagt aacaagagac aacccatctc caagagctta
     5161 tttcaagtgt tcttttgctc ctacctgcct cgtcaaaaag aaggtgcaaa gaagcgtaga
     5221 agatcagtca attctagtgg caacatatga aggagaacac aaccattcac atccctctaa
     5281 aatggacgcc tttacaactt ccccttgtag ccgtttaaac gggaagagta ctcttggggt
     5341 tactgcggca gtgccatgtc atactctcaa cagcacagaa cccaaaaaca tgttacaaat
     5401 agaagataag aaagtggcta gcagtacaag tactagtact gcaggtggac ataagcgcaa
     5461 attattatca ggaggaagtg ataataataa tcatcaaaat agaccagagt ttcagcattt
     5521 tttgatagag caaatggctt cttccttgac aaaagatcca agttttcaag cagccttagc
     5581 tgccgccatt tcgggaaaat tcctaccaaa taatcacaca gctaaacccg gggacccagc
     5641 tttcttgtac aaagtggttg ataacagcgg gttaattaac atcttttacc catacgatgt
     5701 tcctgactat gcgggctatc cctatgacgt cccggactat gcaggatcct atccatatga
     5761 cgttccagat tacgctgctc agtgcagcgc ttagagctcg aatttccccg atcgttcaaa
     5821 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     5881 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     5941 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     6001 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     6061 tcgggaatta gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg agaggaattt
     6121 ccgacgagca cagaaaggac ttgctcttgg acgtaggcct atttctcagg cacatgtatc
     6181 aagtgttcgg acgtgggttt tcgatggtgt atcagccgcc gccaactggg agatgaggag
     6241 gctttcttgg ggggcagtca gcagttcatt tcacaagaca gaggaacttg taaggagatg
     6301 cactgattta tcttggcgca aaccagcagg acgaattagt gggaatagcc cgcgaatatc
     6361 taagttatgc ctgtcggcat gagcagaaac ttccaattcg aaacagtttg gagaggttgt
     6421 ttttgggcat accttttgtt agtcagcctc tcgattgctc atcgtcatta cacagtaccg
     6481 aagtttgatc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     6541 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     6601 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     6661 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     6721 tattgccaaa tgtttgaacg atctgcttcg acgcactcct tctttactcc accatctcgt
     6781 ccttattgaa aacgtgggta gcaccaaaac gaatcaagtc gctggaactg aagttaccaa
     6841 tcacgctgga tgatttgcca gttggattaa tcttgccttt ccccgcatga ataatattga
     6901 tgaatgcatg cgtgaggggt atttcgattt tggcaatagc tgcaattgcc gcgacatcct
     6961 ccaacgagca taattcttca gaaaaatagc gatgttccat gttgtcaggg catgcatgat
     7021 gcacgttatg aggtgacggt gctaggcagt attccctcaa agtttcatag tcagtatcat
     7081 attcatcatt gcattcctgc aagagagaat tgagacgcaa tccacacgct gcggcaacct
     7141 tccggcgttc gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg gatcccggtc
     7201 ggcatctact ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg
     7261 cgagtacttc tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac
     7321 gcccgacagt cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg
     7381 catcatcgaa attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca
     7441 tatacgcccg gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg
     7501 tctgctgctc catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt
     7561 gggaatcccc gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg
     7621 tcaggacatt gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct
     7681 cggcccaaag catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca
     7741 tcacagtttg ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg
     7801 tagtgtattg accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat
     7861 cggccgcagc gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg
     7921 tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc
     7981 tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt
     8041 gccgataaac ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat
     8101 atccacgccc tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca
     8161 tcaggtcgga gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga
     8221 gttcaggctt tttcatatcg gggtcgtcct ctccaaatga aatgaacttc cttatataga
     8281 ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc
     8341 acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct
     8401 cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt
     8461 tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat
     8521 gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg
     8581 aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac
     8641 gagagtgtcg tgctccacca tgttgacgga tctctaggac gcgtcgacgc gtcgacgcgt
     8701 cgacgcgtcg acgcgtccta gaagctaatt cactggccgt cgttttacaa cgtcgtgact
     8761 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     8821 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     8881 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8941 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     9001 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     9061 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     9121 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     9181 gaaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     9241 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     9301 gaaaaaccac cccagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc
     9361 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag
     9421 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg
     9481 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca
     9541 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac
     9601 gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat
     9661 cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga gaactatgcc
     9721 gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc tatttcttta
     9781 gaagtgaacg ttgacgatat caactcccct atccattgct caccgaatgg tacaggtcgg
     9841 ggacccgaag ttccgactgt cggcctgatg catccccggc tgatcgaccc cagatctggg
     9901 gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc cccggaacca
     9961 aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga gaacattggt
    10021 tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga gcagaagtcc
    10081 tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt gccacttgcg
    10141 ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg cgacgccgac
    10201 aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag ttccgcaaat
    10261 agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag agatgaacac
    10321 gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca ggcggtagac
    10381 cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga tgaagatgcg
    10441 catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata aacccgccgg
    10501 caacgcccgc agcagcatac cggcgacccc tcggcctcgc tgttcgggct ccacgaaaac
    10561 gccggacaga tgcgccttgt gagcgtcctt ggggccgtcc tcctgtttga agaccgacag
    10621 cccaatgatc tcgccgtcga tgtaggcgcc gaatgccacg gcatctcgca accgttcagc
    10681 gaacgcctcc atgggctttt tctcctcgtg ctcgtaaacg gacccgaaca tctctggagc
    10741 tttcttcagg gccgacaatc ggatctcgcg gaaatcctgc acgtcggccg ctccaagccg
    10801 tcgaatctga gccttaatca caattgtcaa ttttaatcct ctgtttatcg gcagttcgta
    10861 gagcgcgccg tgcgtcccga gcgatactga gcgaagcaag tgcgtcgagc agtgcccgct
    10921 tgttcctgaa atgccagtaa agcgctggct gctgaacccc cagccggaac tgaccccaca
    10981 aggccctagc gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg
    11041 ctgcctcgca actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg
    11101 gaatccgatc cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc
    11161 gagctgaaat agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat
    11221 atgaatttcg tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc
    11281 tggcaacggg acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc
    11341 gattccaggt gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac
    11401 aggcattcct cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa
    11461 agctcgtaga acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac
    11521 acctgctgcc acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc
    11581 ttcacgtcct tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc
    11641 ttgttgcgcg tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg
    11701 tccggccacg gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg
    11761 tgtttcagca acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg
    11821 gtttttcgct tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa
    11881 cctgccgcct cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg
    11941 gcagggggag ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc
    12001 gagccgacgg actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt
    12061 tcggcatcct cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca
    12121 aacgtccgat tcattcaccc tccttgcggg attgccccga ctcacgccgg ggcaatgtgc
    12181 ccttattcct gatttgaccc gcctggtgcc ttggtgtcca gataatccac cttatcggca
    12241 atgaagtcgg tcccgtagac cgtctggccg tccttctcgt acttggtatt ccgaatcttg
    12301 ccctgcacga ataccagcga ccccttgccc aaatacttgc cgtgggcctc ggcctgagag
    12361 ccaaaacact tgatgcggaa gaagtcggtg cgctcctgct tgtcgccggc atcgttgcgc
    12421 cacatctagg tactaaaaca attcatccag taaaatataa tattttattt tctcccaatc
    12481 aggcttgatc cccagtaagt caaaaaatag ctcgacatac tgttcttccc cgatatcctc
    12541 cctgatcgac cggacgcaga aggcaatgtc ataccacttg tccgccctgc cgcttctccc
    12601 aagatcaata aagccactta ctttgccatc tttcacaaag atgttgctgt ctcccaggtc
    12661 gccgtgggaa aagacaagtt cctcttcggg cttttccgtc tttaaaaaat catacagctc
    12721 gcgcggatct ttaaatggag tgtcttcttc ccagttttcg caatccacat cggccagatc
    12781 gttattcagt aagtaatcca attcggctaa gcggctgtct aagctattcg tatagggaca
    12841 atccgatatg tcgatggagt gaaagagcct gatgcactcc gcatacagct cgataatctt
    12901 ttcagggctt tgttcatctt catactcttc cgagcaaagg acgccatcgg cctcactcat
    12961 gagcagattg ctccagccat catgccgttc aaagtgcagg acctttggaa caggcagctt
    13021 tccttccagc catagcatca tgtccttttc ccgttccaca tcataggtgg tccctttata
    13081 ccggctgtcc gtcattttta aatataggtt ttcattttct cccaccagct tatatacctt
    13141 agcaggagac attccttccg tatcttttac gcagcggtat ttttcgatca gttttttcaa
    13201 ttccggtgat attctcattt tagccattta ttatttcctt cctcttttct acagtattta
    13261 aagatacccc aagaagctaa ttataacaag acgaactcca attcactgtt ccttgcattc
    13321 taaaacctta aataccagaa aacagctttt tcaaagttgt tttcaaagtt ggcgtataac
    13381 atagtatcga cggagccgat tttgaaacca caattatggg tgatgctgcc aacttactga
    13441 tttagtgtat gatggtgttt ttgaggtgct ccagtggctt ctgtttctat cagctgtccc
    13501 tcctgttcag ctactgacgg ggtggtgcgt aacggcaaaa gcaccgccgg acatcagcgc
    13561 tatctctgct ctcactgccg taaaacatgg caactgcagt tcacttacac cgcttctcaa
    13621 cccggtacgc accagaaaat cattgatatg gccatgaatg gcgttggatg ccgggcaaca
    13681 gcccgcatta tgggcgttgg cctcaacacg attttacgtc acttaaaaaa ctcaggccgc
    13741 agtcggtaac ctcgcgcata cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg
    13801 aacagtgggg ctatgtcggg gctaaatcgc gccagcgctg gctgttttac gcgtatgaca
    13861 gtctccggaa gacggttgtt gcgcacgtat tcggtgaacg cactatggcg acgctggggc
    13921 gtcttatgag cctgctgtca ccctttgacg tggtgatatg gatgacggat ggctggccgc
    13981 tgtatgaatc ccgcctgaag ggaaagctgc acgtaatcag caagcgatat acgcagcgaa
    14041 ttgagcggca taacctgaat ctgaggcagc acctggcacg gctgggacgg aagtcgctgt
    14101 cgttctcaaa atcggtggag ctgcatgaca aagtcatcgg gcattatctg aacataaaac
    14161 actatcaata agttggagtc attacccaat tatgatagaa tttacaagct ataaggttat
    14221 tgtcctgggt ttcaagcatt agtccatgca agtttttatg ctttgcccat tctatagata
    14281 tattgataag cgcgctgcct atgccttgcc ccctgaaatc cttacatacg gcgatatctt
    14341 ctatataaaa gatatattat cttatcagta ttgtcaatat attcaaggca atctgcctcc
    14401 tcatcctctt catcctcttc gtcttggtag ctttttaaat atggcgcttc atagagtaat
    14461 tctgtaaagg tccaattctc gttttcatac ctcggtataa tcttacctat cacctcaaat
    14521 ggttcgctgg gtttatcgca cccccgaaca cgagcacggc acccgcgacc actatgccaa
    14581 gaatgcccaa ggtaaaaatt gccggccccg ccatgaagtc cgtgaatgcc ccgacggccg
    14641 aagtgaaggg caggccgcca cccaggccgc cgccctcact gcccggcacc tggtcgctga
    14701 atgtcgatgc cagcacctgc ggcacgtcaa tgcttccggg cgtcgcgctc gggctgatcg
    14761 cccatcccgt tactgccccg atcccggcaa tggcaaggac tgccagcgct gccatttttg
    14821 gggtgaggcc gttcgcggcc gaggggcgca gcccctgggg ggatgggagg cccgcgttag
    14881 cgggccggga gggttcgaga agggggggca ccccccttcg gcgtgcgcgg tcacgcgcac
    14941 agggcgcagc cctggttaaa aacaaggttt ataaatattg gtttaaaagc aggttaaaag
    15001 acaggttagc ggtggccgaa aaacgggcgg aaacccttgc aaatgctgga ttttctgcct
    15061 gtggacagcc cctcaaatgt caataggtgc gcccctcatc tgtcagcact ctgcccctca
    15121 agtgtcaagg atcgcgcccc tcatctgtca gtagtcgcgc ccctcaagtg tcaataccgc
    15181 agggcactta tccccaggct tgtccacatc atctgtggga aactcgcgta aaatcaggcg
    15241 ttttcgccga tttgcgaggc tggccagctc cacgtcgccg gccgaaatcg agcctgcccc
    15301 tcatctgtca acgccgcgcc gggtgagtcg gcccctcaag tgtcaacgtc cgcccctcat
    15361 ctgtcagtga gggccaagtt ttccgcgagg tatccacaac gccggcggcc gcggtgtctc
    15421 gcacacggct tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca
    15481 gcggcgaggg caaccagccc ggtgagcgtc ggaaaggcgc
//
