LOCUS       sequence-247578-        7850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV packaging vector for CamKIIa driven KOR DREADD expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Vardy E, Robinson JE, Li C, Olsen RH, DiBerto JF, Giguere PM, 
            Sassano FM, Huang XP, Zhu H, Urban DJ, White KL, Rittiner JE, 
            Crowley NA, Pleil KE, Mazzone CM, Mosier PD, Song J, Kash TL, 
            Malanga CJ, Krashes MJ, Roth BL
  TITLE     A New DREADD Facilitates the Multiplexed Chemogenetic Interrogation 
            of Behavior.
  JOURNAL   Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065.
  PUBMED    25937170
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 
            10.1016/j.neuron.2015.03.065."
COMMENT     Sequence Label: pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
FEATURES             Location/Qualifiers
     source          1..7850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        149..1437
                     /label=CaMKII promoter
                     /note="promoter for the mouse calcium/calmodulin-dependent 
                     kinase II alpha subunit"
     CDS             1465..1491
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(2125..2136)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    2650..3223
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2817..2834)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3044..3063
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      3224..3233
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3230..3232,3233..3235,3236..3952)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKC"
     primer_bind     complement(3275..3296)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3536..3555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3883..3904
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3977..4565
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4030..4050)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4448..4459)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4597..5073
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4796..4815)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   5113..5253
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   5113..5242
                     /label=AAV2 ITR
     rep_origin      5328..5783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5415..5434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5625..5646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5800..5819)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5919..5941
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5979..5997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6065..6169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6170..6238,6239..7030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6388..6407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7201..7789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7690..7709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtttaaca ttatggcctt aggtcacttc atctccatgg
      181 ggttcttctt ctgattttct agaaaatgag atgggggtgc agagagcttc ctcagtgacc
      241 tgcccagggt cacatcagaa atgtcagagc tagaacttga actcagatta ctaatcttaa
      301 attccatgcc ttgggggcat gcaagtacga tatacagaag gagtgaactc attagggcag
      361 atgaccaatg agtttaggaa agaagagtcc agggcagggt acatctacac cacccgccca
      421 gccctgggtg agtccagcca cgttcacctc attatagttg cctctctcca gtcctacctt
      481 gacgggaagc acaagcagaa actgggacag gagccccagg agaccaaatc ttcatggtcc
      541 ctctgggagg atgggtgggg agagctgtgg cagaggcctc aggaggggcc ctgctgctca
      601 gtggtgacag ataggggtga gaaagcagac agagtcattc cgtcagcatt ctgggtctgt
      661 ttggtacttc ttctcacgct aaggtggcgg tgtgatatgc acaatggcta aaaagcaggg
      721 agagctggaa agaaacaagg acagagacag aggccaagtc aaccagacca attcccagag
      781 gaagcaaaga aaccattaca gagactacaa gggggaaggg aaggagagat gaattagctt
      841 cccctgtaaa ccttagaacc cagctgttgc cagggcaacg gggcaatacc tgtctcttca
      901 gaggagatga agttgccagg gtaactacat cctgtctttc tcaaggacca tcccagaatg
      961 tggcacccac tagccgttac catagcaact gcctctttgc cccacttaat cccatcccgt
     1021 ctgttaaaag ggccctatag ttggaggtgg gggaggtagg aagagcgatg atcacttgtg
     1081 gactaagttt gttcgcatcc ccttctccaa ccccctcagt acatcaccct gggggaacag
     1141 ggtccacttg ctcctgggcc cacacagtcc tgcagtattg tgtatataag gccagggcaa
     1201 agaggagcag gttttaaagt gaaaggcagg caggtgttgg ggaggcagtt accggggcaa
     1261 cgggaacagg gcgtttcgga ggtggttgcc atggggacct ggatgctgac gaaggctcgc
     1321 gaggctgtga gcagccacag tgccctgctc agaagcccca agctcgtcag tcaagccggt
     1381 tctccgtttg cactcaggag cacgggcagg cgagtggccc ctagttctgg gggcagctct
     1441 agagcggtac cggatcccac catgtaccca tacgatgttc cagattacgc tatggaatcc
     1501 ccgattcaga tcttccgcgg ggagccgggc cctacctgcg ccccgagcgc ctgcctgccc
     1561 cccaacagca gcgcctggtt tcccggctgg gccgagcccg acagcaacgg cagcgccggc
     1621 tcggaggacg cgcagctgga gcccgcgcac atctccccgg ccatcccggt catcatcacg
     1681 gcggtctact ccgtagtgtt cgtcgtgggc ttggtgggca actcgctggt catgttcgtg
     1741 atcatccgat acacaaagat gaagacagca accaacattt acatatttaa cctggctttg
     1801 gcagatgctt tagttactac aaccatgccc tttcagagta cggtctactt gatgaactcc
     1861 tggccttttg gggatgtgct gtgcaagata gtaatttcca ttaattacta caacatgttc
     1921 accagcatct tcaccttgac catgatgagc gtggaccgct acattgccgt gtgccacccc
     1981 gtgaaggctt tggacttccg cacacccttg aaggcaaaga tcatcaatat ctgcatctgg
     2041 ctgctgtcgt catctgttgg catctctgca atagtccttg gaggcaccaa agtcagggaa
     2101 gacgtcgatg tcattgagtg ctccttgcag ttcccagatg atgactactc ctggtgggac
     2161 ctcttcatga agatctgcgt cttcatcttt gccttcgtga tccctgtcct catcatcatc
     2221 gtctgctaca ccctgatgat cctgcgtctc aagagcgtcc ggctcctttc tggctcccga
     2281 gagaaagatc gcaacctgcg taggatcacc agactggtcc tggtggtggt ggcagtcttc
     2341 gtcgtctgct ggactcccat tcacatattc atcctggtgg aggctctggg gagcacctcc
     2401 cacagcacag ctgctctctc cagctattac ttctgcatcg ccttaggcta taccaacagt
     2461 agcctgaatc ccattctcta cgcctttctt gatgaaaact tcaagcggtg tttccgggac
     2521 ttctgctttc cactgaagat gaggatggag cggcagagca ctagcagagt ccgaaataca
     2581 gttcaggacc ctgcttacct gagggacatc gatgggatga ataaaccagt atgagcggcc
     2641 gcgatatcgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     2701 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2761 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2821 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2881 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2941 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     3001 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     3061 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     3121 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     3181 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagccacca tggtgagcaa
     3241 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     3301 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     3361 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     3421 cttcggctac ggcctgatgt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
     3481 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     3541 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     3601 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     3661 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     3721 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     3781 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
     3841 ccagtccaaa ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     3901 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtgct aggaattcga
     3961 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     4021 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     4081 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     4141 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     4201 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccggaacttt
     4261 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4321 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4381 ctttccttgg ctgctcgcct atgttgccac ctggattctg cgcgggacgt ccttctgcta
     4441 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4501 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4561 cccgcatcga taccgagcgc tgctcgagag atctacgggt ggcatccctg tgacccctcc
     4621 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata
     4681 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg
     4741 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat
     4801 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg
     4861 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc
     4921 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg
     4981 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta
     5041 caggcgtgaa ccactgctcc cttccctgtc cttctgattt tgtaggtaac cacgtgcgga
     5101 ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     5161 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     5221 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     5281 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     5341 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5401 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5461 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5521 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     5581 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5641 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     5701 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5761 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     5821 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5881 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     5941 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     6001 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     6061 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     6121 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     6181 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6241 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6301 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6361 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6421 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6481 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6541 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6601 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6661 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6721 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6781 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6841 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6901 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6961 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     7021 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     7081 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     7141 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     7201 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7261 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7321 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7381 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7441 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7501 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7561 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7621 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7681 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7741 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7801 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
