LOCUS       sequence-24758-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Mao T, O'Connor DH, Scheuss V, Nakai J, Svoboda K
  TITLE     Characterization and subcellular targeting of GCaMP-type 
            genetically-encoded calcium indicators.
  JOURNAL   PLoS ONE. 2008  . 3(3):e1796.
  PUBMED    18350138
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2008  . 3(3):e1796."
COMMENT     Sequence Label: pCAGGS-GCaMP2
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             177..194
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             198..230
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(234..242,243..257)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      264..273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             285..341
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     554..575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(681..702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggctncntgc tacatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
       61 gttattgtgc tgtctcatca ttttggcaaa gaattcggta ccgcgggccc ggcgcgccag
      121 ctggatatct ccggacccgg gctagcgcaa ttgcggccgc caccatgcgg ggttctcatc
      181 atcatcatca tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt
      241 acgacgatga cgataaggat ctcgccacca tggtcgactc atcacgtcgt aagtggaata
      301 agacaggtca cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca
      361 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
      421 acggcggcgt gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg
      481 tgctgctgcc cgacaaccac tacctgagca cccagtccaa actttcgaaa gaccccaacg
      541 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      601 tggacgagct gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt
      661 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      721 gcgtgtccgg cgagggtgan ggcgatgcca cct
//
