LOCUS       sequence-247583-       16648 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16648)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16648)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX169
FEATURES             Location/Qualifiers
     source          1..16648
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6762..6786)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6815..6904
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      6938..7190
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7619..7871)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8339..9364)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8339..9364)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9374..9718)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(9860..9877)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9860..9876)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9869..9891)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10016..10035)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10226..10247
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10494..10518
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12114..13262)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13561..14355)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14547..15314
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16007..16638
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca ctagtatgga aaaggaaaac aaactaaaat cagagacttc
     5881 attggaaaat ccgatgtttt cacaacagat tcaaactgaa aaggattctt taggtttttt
     5941 agacaacatg tttggtgctc atcttcttga tttcaatact actattcctt ccattttcga
     6001 tttgcttcaa acttcaccac ctcaatcact gactaatatt ccatctccaa cttccacatt
     6061 tcaagaatca tgtgaggtgg aggtggtgaa ttcactgtcg atctcttcat catcaactga
     6121 agctgccgaa gatgatcagc agagtaatac agtaaagcaa caagatgaag atgaagacaa
     6181 gtataagaaa cagttaaaac ctaaaaagaa gaatgttcaa aagaggcaaa gagagccaag
     6241 atttgcattc atgaccaaga gcgaaattga tcatttggat gatggtttta gatggaggaa
     6301 atatggccaa aaagcagtga aaaacagccc ctttccaagg agctattatc gttgcacaac
     6361 tgtatcatgt ggagtaaaaa agagagtgga aaggtcaatc aaagatactt caatagttgt
     6421 tacaacttac gaaggtactc acacacatcc gtgccgcatt actccgtcgc gcgggagcat
     6481 tggcggcgtt ctgccacgaa ccaccgccta ctacggtggc gccattggtg gtggtaatgg
     6541 tggaggagga acttgtagta attcctcttt gtctatccca caacttcaat attatcaaga
     6601 acagcagcag caaatgctcc attcacttac tacaacttca cctttaaata actttaatgt
     6661 tggttactct tccttatttt caacaaattc atttcaagac cacgggcttc ttcatgatat
     6721 ggtgtcatca caaagagaga cagattcaaa attacccggg gacccagctt tcttgtacaa
     6781 agtggttgat aacagcgggt taattaacat cttttaccca tacgatgttc ctgactatgc
     6841 gggctatccc tatgacgtcc cggactatgc aggatcctat ccatatgacg ttccagatta
     6901 cgctgctcag tgcagcgctt agagctcgaa tttccccgat cgttcaaaca tttggcaata
     6961 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     7021 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     7081 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     7141 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc gggaattagc
     7201 ttcatcaacg caagacatgc gcacgaccgt ctgacaggag aggaatttcc gacgagcaca
     7261 gaaaggactt gctcttggac gtaggcctat ttctcaggca catgtatcaa gtgttcggac
     7321 gtgggttttc gatggtgtat cagccgccgc caactgggag atgaggaggc tttcttgggg
     7381 ggcagtcagc agttcatttc acaagacaga ggaacttgta aggagatgca ctgatttatc
     7441 ttggcgcaaa ccagcaggac gaattagtgg gaatagcccg cgaatatcta agttatgcct
     7501 gtcggcatga gcagaaactt ccaattcgaa acagtttgga gaggttgttt ttgggcatac
     7561 cttttgttag tcagcctctc gattgctcat cgtcattaca cagtaccgaa gtttgatcga
     7621 tctagtaaca tagatgacac cgcgcgcgat aatttatcct agtttgcgcg ctatattttg
     7681 ttttctatcg cgtattaaat gtataattgc gggactctaa tcataaaaac ccatctcata
     7741 aataacgtca tgcattacat gttaattatt acatgcttaa cgtaattcaa cagaaattat
     7801 atgataatca tcgcaagacc ggcaacagga ttcaatctta agaaacttta ttgccaaatg
     7861 tttgaacgat ctgcttcgac gcactccttc tttactccac catctcgtcc ttattgaaaa
     7921 cgtgggtagc accaaaacga atcaagtcgc tggaactgaa gttaccaatc acgctggatg
     7981 atttgccagt tggattaatc ttgcctttcc ccgcatgaat aatattgatg aatgcatgcg
     8041 tgaggggtat ttcgattttg gcaatagctg caattgccgc gacatcctcc aacgagcata
     8101 attcttcaga aaaatagcga tgttccatgt tgtcagggca tgcatgatgc acgttatgag
     8161 gtgacggtgc taggcagtat tccctcaaag tttcatagtc agtatcatat tcatcattgc
     8221 attcctgcaa gagagaattg agacgcaatc cacacgctgc ggcaaccttc cggcgttcgt
     8281 ggtctatttg ctcttggacg ttgcaaacgt aagtgttgga tcccggtcgg catctactct
     8341 attcctttgc cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta
     8401 cacagccatc ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc
     8461 cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat
     8521 tgccgtcaac caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga
     8581 gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca
     8641 tacaagccaa ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga
     8701 acatcgcctc gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt
     8761 tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca
     8821 tcagctcatc gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc
     8881 agtgatacac atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac
     8941 cgattccttg cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga
     9001 tcgcatccat ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt
     9061 cttgcaacgt gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt
     9121 ccccaatgtc aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat
     9181 aacgatcttt gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc
     9241 ctacatcgaa gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga
     9301 cgctgtcgaa cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt
     9361 tcatatcggg gtcgtcctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg
     9421 cgaaggatag tgggattgtg cgtcatccct tacgtcagtg gagatatcac atcaatccac
     9481 ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg
     9541 tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca
     9601 atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat
     9661 agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat
     9721 agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg
     9781 ctccaccatg ttgacggatc tctaggacgc gtcgacgcgt cgacgcgtcg acgcgtcgac
     9841 gcgtcctaga agctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     9901 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     9961 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gcccgctcct
    10021 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
    10081 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
    10141 gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
    10201 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
    10261 cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga accaccatca
    10321 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
    10381 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
    10441 cagtacatta aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca
    10501 ccacaatata tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa
    10561 tcaccactcg atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta
    10621 aggcggcaga ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg
    10681 ggtttgaaac acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg
    10741 ttgctgcctg tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat
    10801 tcatttctcg cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga
    10861 aatcgctgga taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt
    10921 gacgatatca actcccctat ccattgctca ccgaatggta caggtcgggg acccgaagtt
    10981 ccgactgtcg gcctgatgca tccccggctg atcgacccca gatctggggc tgagaaagcc
    11041 cagtaaggaa acaactgtag gttcgagtcg cgagatcccc cggaaccaaa ggaagtaggt
    11101 taaacccgct ccgatcaggc cgagccacgc caggccgaga acattggttc ctgtaggcat
    11161 cgggattggc ggatcaaaca ctaaagctac tggaacgagc agaagtcctc cggccgccag
    11221 ttgccaggcg gtaaaggtga gcagaggcac gggaggttgc cacttgcggg tcagcacggt
    11281 tccgaacgcc atggaaaccg cccccgccag gcccgctgcg acgccgacag gatctagcgc
    11341 tgcgtttggt gtcaacacca acagcgccac gcccgcagtt ccgcaaatag cccccaggac
    11401 cgccatcaat cgtatcgggc tacctagcag agcggcagag atgaacacga ccatcagcgg
    11461 ctgcacagcg cctaccgtcg ccgcgacccc gcccggcagg cggtagaccg aaataaacaa
    11521 caagctccag aatagcgaaa tattaagtgc gccgaggatg aagatgcgca tccaccagat
    11581 tcccgttgga atctgtcgga cgatcatcac gagcaataaa cccgccggca acgcccgcag
    11641 cagcataccg gcgacccctc ggcctcgctg ttcgggctcc acgaaaacgc cggacagatg
    11701 cgccttgtga gcgtccttgg ggccgtcctc ctgtttgaag accgacagcc caatgatctc
    11761 gccgtcgatg taggcgccga atgccacggc atctcgcaac cgttcagcga acgcctccat
    11821 gggctttttc tcctcgtgct cgtaaacgga cccgaacatc tctggagctt tcttcagggc
    11881 cgacaatcgg atctcgcgga aatcctgcac gtcggccgct ccaagccgtc gaatctgagc
    11941 cttaatcaca attgtcaatt ttaatcctct gtttatcggc agttcgtaga gcgcgccgtg
    12001 cgtcccgagc gatactgagc gaagcaagtg cgtcgagcag tgcccgcttg ttcctgaaat
    12061 gccagtaaag cgctggctgc tgaaccccca gccggaactg accccacaag gccctagcgt
    12121 ttgcaatgca ccaggtcatc attgacccag gcgtgttcca ccaggccgct gcctcgcaac
    12181 tcttcgcagg cttcgccgac ctgctcgcgc cacttcttca cgcgggtgga atccgatccg
    12241 cacatgaggc ggaaggtttc cagcttgagc gggtacggct cccggtgcga gctgaaatag
    12301 tcgaacatcc gtcgggccgt cggcgacagc ttgcggtact tctcccatat gaatttcgtg
    12361 tagtggtcgc cagcaaacag cacgacgatt tcctcgtcga tcaggacctg gcaacgggac
    12421 gttttcttgc cacggtccag gacgcggaag cggtgcagca gcgacaccga ttccaggtgc
    12481 ccaacgcggt cggacgtgaa gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg
    12541 gccttcgtgt aataccggcc attgatcgac cagcccaggt cctggcaaag ctcgtagaac
    12601 gtgaaggtga tcggctcgcc gataggggtg cgcttcgcgt actccaacac ctgctgccac
    12661 accagttcgt catcgtcggc ccgcagctcg acgccggtgt aggtgatctt cacgtccttg
    12721 ttgacgtgga aaatgacctt gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg
    12781 gtgaacaggg cagagcgggc cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc
    12841 gcaatatcga acaaggaaag ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac
    12901 gcggcctgct tggcctcgct gacctgtttt gccaggtcct cgccggcggt ttttcgcttc
    12961 ttggtcgtca tagttcctcg cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc
    13021 tgttcgagac gacgcgaacg ctccacggcg gccgatggcg cgggcagggc agggggagcc
    13081 agttgcacgc tgtcgcgctc gatcttggcc gtagcttgct ggaccatcga gccgacggac
    13141 tggaaggttt cgcggggcgc acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg
    13201 gcggaaaacc ccgcgtcgat cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc
    13261 attcaccctc cttgcgggat tgccccgact cacgccgggg caatgtgccc ttattcctga
    13321 tttgacccgc ctggtgcctt ggtgtccaga taatccacct tatcggcaat gaagtcggtc
    13381 ccgtagaccg tctggccgtc cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat
    13441 accagcgacc ccttgcccaa atacttgccg tgggcctcgg cctgagagcc aaaacacttg
    13501 atgcggaaga agtcggtgcg ctcctgcttg tcgccggcat cgttgcgcca catctaggta
    13561 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
    13621 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
    13681 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
    13741 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
    13801 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
    13861 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
    13921 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
    13981 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
    14041 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
    14101 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
    14161 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
    14221 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
    14281 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
    14341 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
    14401 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
    14461 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
    14521 gagccgattt tgaaaccaca attatgggtg atgctgccaa cttactgatt tagtgtatga
    14581 tggtgttttt gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct
    14641 actgacgggg tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct
    14701 cactgccgta aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac
    14761 cagaaaatca ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg
    14821 ggcgttggcc tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct
    14881 cgcgcataca gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct
    14941 atgtcggggc taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga
    15001 cggttgttgc gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc
    15061 tgctgtcacc ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc
    15121 gcctgaaggg aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata
    15181 acctgaatct gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat
    15241 cggtggagct gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag
    15301 ttggagtcat tacccaatta tgatagaatt tacaagctat aaggttattg tcctgggttt
    15361 caagcattag tccatgcaag tttttatgct ttgcccattc tatagatata ttgataagcg
    15421 cgctgcctat gccttgcccc ctgaaatcct tacatacggc gatatcttct atataaaaga
    15481 tatattatct tatcagtatt gtcaatatat tcaaggcaat ctgcctcctc atcctcttca
    15541 tcctcttcgt cttggtagct ttttaaatat ggcgcttcat agagtaattc tgtaaaggtc
    15601 caattctcgt tttcatacct cggtataatc ttacctatca cctcaaatgg ttcgctgggt
    15661 ttatcgcacc cccgaacacg agcacggcac ccgcgaccac tatgccaaga atgcccaagg
    15721 taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa gtgaagggca
    15781 ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat gtcgatgcca
    15841 gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc catcccgtta
    15901 ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg gtgaggccgt
    15961 tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg ggccgggagg
    16021 gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag ggcgcagccc
    16081 tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac aggttagcgg
    16141 tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt ggacagcccc
    16201 tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag tgtcaaggat
    16261 cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag ggcacttatc
    16321 cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt ttcgccgatt
    16381 tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc atctgtcaac
    16441 gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct gtcagtgagg
    16501 gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc acacggcttc
    16561 gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc ggcgagggca
    16621 accagcccgg tgagcgtcgg aaaggcgc
//
