LOCUS       sequence-247588-       15574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15574)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX173
FEATURES             Location/Qualifiers
     source          1..15574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5688..5712)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5741..5830
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5864..6116
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6545..6797)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7265..8290)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7265..8290)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8300..8644)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8786..8803)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8786..8802)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8795..8817)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8942..8961)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9152..9173
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9420..9444
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(11040..12188)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12487..13281)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13473..14240
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14933..15564
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggaatt
     4621 cacaagtttg gttgatactt ccttggattt gagttttaga cctcttccag ttcttgataa
     4681 agtggcgaaa ctagaagtgc agagtaattt cactggattg agcagggaca atatgctggt
     4741 gaaagatgag gctggtgatt tgttagagga actaaacaga gtgagcagtg aaaacaagaa
     4801 gctaacagag atgctcacag tagtgtgtga aaattacaat gctttaagaa accaattaat
     4861 ggaatatatg aacaaccagc agaataatgg tgtagtagat gatagtgctg gatcaaggaa
     4921 aagaaaatct gaaaatctct ccaatcccaa caacaacaac aaaaacaaca actcggatat
     4981 tgtttgtgga cgttcatcag aaagcagctc aagtgatgaa gagtcttgtt gcaaaaaacc
     5041 tagagaagaa cacataaaaa ctaaggtttc tgtcgtttct acgaagacag aagcatctta
     5101 tacctctctt attgtaaagg atggttatca gtggaggaaa tatggtcaga aagtaactag
     5161 agacaatcct tctccgagag cttacttcag gtgctctttt gctcctggct gccccgtcaa
     5221 gaaaaaggtg caaagaagca tagaagatca gtcaattgtg gtggcaacat atgaaggaga
     5281 acataaccat ccaatacacc cttcaaaacc agaggcagct gctggtactt ctactacttc
     5341 caccggcagc cgtttaaatg tgagaactat tgggggtacg actgcttcag tcccttgctc
     5401 taccactctc aatccctcag gaccaaccat tactctcgat cttactgaac ctaaaacagt
     5461 agcaaaaggc gatatcatga agatgagtag cagtattagt cctaaaggtg gcaatagcca
     5521 caggacaaca gaaggtgatc actatagtag gccagagttt caacagttct tgatagagca
     5581 aatggcttct tcattgacta aagatccaat tttcaaagca gcacttgctg ccgccatttc
     5641 aggaaaaaat ctccaacata ataatcagac aagtagatgg cccggggacc cagctttctt
     5701 gtacaaagtg gttgataaca gcgggttaat taacatcttt tacccatacg atgttcctga
     5761 ctatgcgggc tatccctatg acgtcccgga ctatgcagga tcctatccat atgacgttcc
     5821 agattacgct gctcagtgca gcgcttagag ctcgaatttc cccgatcgtt caaacatttg
     5881 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     5941 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     6001 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     6061 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga
     6121 attagcttca tcaacgcaag acatgcgcac gaccgtctga caggagagga atttccgacg
     6181 agcacagaaa ggacttgctc ttggacgtag gcctatttct caggcacatg tatcaagtgt
     6241 tcggacgtgg gttttcgatg gtgtatcagc cgccgccaac tgggagatga ggaggctttc
     6301 ttggggggca gtcagcagtt catttcacaa gacagaggaa cttgtaagga gatgcactga
     6361 tttatcttgg cgcaaaccag caggacgaat tagtgggaat agcccgcgaa tatctaagtt
     6421 atgcctgtcg gcatgagcag aaacttccaa ttcgaaacag tttggagagg ttgtttttgg
     6481 gcataccttt tgttagtcag cctctcgatt gctcatcgtc attacacagt accgaagttt
     6541 gatcgatcta gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat
     6601 attttgtttt ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat
     6661 ctcataaata acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga
     6721 aattatatga taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc
     6781 caaatgtttg aacgatctgc ttcgacgcac tccttcttta ctccaccatc tcgtccttat
     6841 tgaaaacgtg ggtagcacca aaacgaatca agtcgctgga actgaagtta ccaatcacgc
     6901 tggatgattt gccagttgga ttaatcttgc ctttccccgc atgaataata ttgatgaatg
     6961 catgcgtgag gggtatttcg attttggcaa tagctgcaat tgccgcgaca tcctccaacg
     7021 agcataattc ttcagaaaaa tagcgatgtt ccatgttgtc agggcatgca tgatgcacgt
     7081 tatgaggtga cggtgctagg cagtattccc tcaaagtttc atagtcagta tcatattcat
     7141 cattgcattc ctgcaagaga gaattgagac gcaatccaca cgctgcggca accttccggc
     7201 gttcgtggtc tatttgctct tggacgttgc aaacgtaagt gttggatccc ggtcggcatc
     7261 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     7321 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     7381 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     7441 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     7501 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     7561 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     7621 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     7681 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     7741 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     7801 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     7861 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     7921 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     7981 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     8041 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     8101 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     8161 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     8221 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     8281 gctttttcat atcggggtcg tcctctccaa atgaaatgaa cttccttata tagaggaagg
     8341 gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca
     8401 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
     8461 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
     8521 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
     8581 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
     8641 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
     8701 gtcgtgctcc accatgttga cggatctcta ggacgcgtcg acgcgtcgac gcgtcgacgc
     8761 gtcgacgcgt cctagaagct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     8821 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     8881 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgccc
     8941 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9001 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9061 aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     9121 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     9181 ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggaacca
     9241 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
     9301 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
     9361 ccaccccagt acattaaaaa cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg
     9421 tttacaccac aatatatcct gccaccagcc agccaacagc tccccgaccg gcagctcggc
     9481 acaaaatcac cactcgatac aggcagccca tcagtccggg acggcgtcag cgggagagcc
     9541 gttgtaaggc ggcagacttt gctcatgtta ccgatgctat tcggaagaac ggcaactaag
     9601 ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc atgttgattg taacgatgac
     9661 agagcgttgc tgcctgtgat caaatatcat ctccctcgca gagatccgaa ttatcagcct
     9721 tcttattcat ttctcgctta accgtgacag gctgtcgatc ttgagaacta tgccgacata
     9781 ataggaaatc gctggataaa gccgctgagg aagctgagtg gcgctatttc tttagaagtg
     9841 aacgttgacg atatcaactc ccctatccat tgctcaccga atggtacagg tcggggaccc
     9901 gaagttccga ctgtcggcct gatgcatccc cggctgatcg accccagatc tggggctgag
     9961 aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag atcccccgga accaaaggaa
    10021 gtaggttaaa cccgctccga tcaggccgag ccacgccagg ccgagaacat tggttcctgt
    10081 aggcatcggg attggcggat caaacactaa agctactgga acgagcagaa gtcctccggc
    10141 cgccagttgc caggcggtaa aggtgagcag aggcacggga ggttgccact tgcgggtcag
    10201 cacggttccg aacgccatgg aaaccgcccc cgccaggccc gctgcgacgc cgacaggatc
    10261 tagcgctgcg tttggtgtca acaccaacag cgccacgccc gcagttccgc aaatagcccc
    10321 caggaccgcc atcaatcgta tcgggctacc tagcagagcg gcagagatga acacgaccat
    10381 cagcggctgc acagcgccta ccgtcgccgc gaccccgccc ggcaggcggt agaccgaaat
    10441 aaacaacaag ctccagaata gcgaaatatt aagtgcgccg aggatgaaga tgcgcatcca
    10501 ccagattccc gttggaatct gtcggacgat catcacgagc aataaacccg ccggcaacgc
    10561 ccgcagcagc ataccggcga cccctcggcc tcgctgttcg ggctccacga aaacgccgga
    10621 cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt ttgaagaccg acagcccaat
    10681 gatctcgccg tcgatgtagg cgccgaatgc cacggcatct cgcaaccgtt cagcgaacgc
    10741 ctccatgggc tttttctcct cgtgctcgta aacggacccg aacatctctg gagctttctt
    10801 cagggccgac aatcggatct cgcggaaatc ctgcacgtcg gccgctccaa gccgtcgaat
    10861 ctgagcctta atcacaattg tcaattttaa tcctctgttt atcggcagtt cgtagagcgc
    10921 gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc gagcagtgcc cgcttgttcc
    10981 tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg gaactgaccc cacaaggccc
    11041 tagcgtttgc aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct
    11101 cgcaactctt cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc
    11161 gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg
    11221 aaatagtcga acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat
    11281 ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa
    11341 cgggacgttt tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc
    11401 aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat
    11461 tcctcggcct tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg
    11521 tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc
    11581 tgccacacca gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg
    11641 tccttgttga cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg
    11701 cgcgtggtga acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc
    11761 cacggcgcaa tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc
    11821 agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt
    11881 cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc
    11941 gcctcctgtt cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg
    12001 ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg
    12061 acggactgga aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca
    12121 tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc
    12181 cgattcattc accctccttg cgggattgcc ccgactcacg ccggggcaat gtgcccttat
    12241 tcctgatttg acccgcctgg tgccttggtg tccagataat ccaccttatc ggcaatgaag
    12301 tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg tattccgaat cttgccctgc
    12361 acgaatacca gcgacccctt gcccaaatac ttgccgtggg cctcggcctg agagccaaaa
    12421 cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc cggcatcgtt gcgccacatc
    12481 taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc aatcaggctt
    12541 gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat cctccctgat
    12601 cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc tcccaagatc
    12661 aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca ggtcgccgtg
    12721 ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca gctcgcgcgg
    12781 atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca gatcgttatt
    12841 cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg gacaatccga
    12901 tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa tcttttcagg
    12961 gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac tcatgagcag
    13021 attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca gctttccttc
    13081 cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt tataccggct
    13141 gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata ccttagcagg
    13201 agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt tcaattccgg
    13261 tgatattctc attttagcca tttattattt ccttcctctt ttctacagta tttaaagata
    13321 ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc attctaaaac
    13381 cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta taacatagta
    13441 tcgacggagc cgattttgaa accacaatta tgggtgatgc tgccaactta ctgatttagt
    13501 gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt
    13561 tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctatctc
    13621 tgctctcact gccgtaaaac atggcaactg cagttcactt acaccgcttc tcaacccggt
    13681 acgcaccaga aaatcattga tatggccatg aatggcgttg gatgccgggc aacagcccgc
    13741 attatgggcg ttggcctcaa cacgatttta cgtcacttaa aaaactcagg ccgcagtcgg
    13801 taacctcgcg catacagccg ggcagtgacg tcatcgtctg cgcggaaatg gacgaacagt
    13861 ggggctatgt cggggctaaa tcgcgccagc gctggctgtt ttacgcgtat gacagtctcc
    13921 ggaagacggt tgttgcgcac gtattcggtg aacgcactat ggcgacgctg gggcgtctta
    13981 tgagcctgct gtcacccttt gacgtggtga tatggatgac ggatggctgg ccgctgtatg
    14041 aatcccgcct gaagggaaag ctgcacgtaa tcagcaagcg atatacgcag cgaattgagc
    14101 ggcataacct gaatctgagg cagcacctgg cacggctggg acggaagtcg ctgtcgttct
    14161 caaaatcggt ggagctgcat gacaaagtca tcgggcatta tctgaacata aaacactatc
    14221 aataagttgg agtcattacc caattatgat agaatttaca agctataagg ttattgtcct
    14281 gggtttcaag cattagtcca tgcaagtttt tatgctttgc ccattctata gatatattga
    14341 taagcgcgct gcctatgcct tgccccctga aatccttaca tacggcgata tcttctatat
    14401 aaaagatata ttatcttatc agtattgtca atatattcaa ggcaatctgc ctcctcatcc
    14461 tcttcatcct cttcgtcttg gtagcttttt aaatatggcg cttcatagag taattctgta
    14521 aaggtccaat tctcgttttc atacctcggt ataatcttac ctatcacctc aaatggttcg
    14581 ctgggtttat cgcacccccg aacacgagca cggcacccgc gaccactatg ccaagaatgc
    14641 ccaaggtaaa aattgccggc cccgccatga agtccgtgaa tgccccgacg gccgaagtga
    14701 agggcaggcc gccacccagg ccgccgccct cactgcccgg cacctggtcg ctgaatgtcg
    14761 atgccagcac ctgcggcacg tcaatgcttc cgggcgtcgc gctcgggctg atcgcccatc
    14821 ccgttactgc cccgatcccg gcaatggcaa ggactgccag cgctgccatt tttggggtga
    14881 ggccgttcgc ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc
    14941 gggagggttc gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg
    15001 cagccctggt taaaaacaag gtttataaat attggtttaa aagcaggtta aaagacaggt
    15061 tagcggtggc cgaaaaacgg gcggaaaccc ttgcaaatgc tggattttct gcctgtggac
    15121 agcccctcaa atgtcaatag gtgcgcccct catctgtcag cactctgccc ctcaagtgtc
    15181 aaggatcgcg cccctcatct gtcagtagtc gcgcccctca agtgtcaata ccgcagggca
    15241 cttatcccca ggcttgtcca catcatctgt gggaaactcg cgtaaaatca ggcgttttcg
    15301 ccgatttgcg aggctggcca gctccacgtc gccggccgaa atcgagcctg cccctcatct
    15361 gtcaacgccg cgccgggtga gtcggcccct caagtgtcaa cgtccgcccc tcatctgtca
    15421 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
    15481 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
    15541 agggcaacca gcccggtgag cgtcggaaag gcgc
//
