LOCUS       sequence-247595-         923 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses H258A mutant of PfeE in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Perraud Q, Moynie L, Gasser V, Munier M, Godet J, Hoegy F, Mely Y, 
            Mislin GLA, Naismith JH, Schalk IJ
  TITLE     A Key Role for the Periplasmic PfeE Esterase in Iron Acquisition via
            the Siderophore Enterobactin in Pseudomonas aeruginosa.
  JOURNAL   ACS Chem Biol. 2018 Sep 21;13(9):2603-2614. doi: 
            10.1021/acschembio.8b00543. Epub 2018 Aug 22.
  PUBMED    30086222
REFERENCE   2  (bases 1 to 923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.8b00543"; journalName: "ACS Chem Biol"; date: 
            "2018-09-21- 21"; volume: "13"; issue: "9"; pages: "2603-2614"
COMMENT     Sequence Label: pBADHISTEV_PfeEH258A
FEATURES             Location/Qualifiers
     source          1..923
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             52..72
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atgagctact accatcacca tcaccatcac gattacgaca tcccaacgac cgaaaacctg
       61 tattttcagg gcgccatgaa tccggacccc gaggcgacca tggaccgcag cctgctgcaa
      121 cgccaggacc tgccctatcg cttcagcgcc gtggacctgg actccgtcga tggccagcgg
      181 cattaccggc tgtggctcgg ccgaccgctg caggcaccgc ccgccgccgg ctacccggtg
      241 gtctggatgc tcgacggcaa cgccgccgtc ggcgccctcg acgagtcaac cctgagacgc
      301 ctggccgacg gcgacgcacc gctactggtc gccatcggct accgcacgcc gctgcgcatc
      361 gaccgcgccg ggcgtacctt cgactacacc cccgcgagtc ccggccaggc cgatcagcgc
      421 gacccgctca acggcctgcc cagcggtggc gccgacgcct tcctcgacct gctgcgcgac
      481 ggcatgcgcc cggccgtggc ggcgcaggcg cctctggaca ctgcgcgaca aaccctctgg
      541 ggacactcct acggcggctt gctggtgctc cacgcactgt tcacccgtcc cggcgagttc
      601 gcccgctacg ccgcggccag cccgtcgctg tggtggcgcg acggggcgat cctcggcgaa
      661 cgagcggggc tcgaacagcg cctgcgcgga aagcgcgcgg aactcctgct ctggcgcggc
      721 agcgccgaac cggccagccc gcgcggctcg ctgaaagccg agccgggcca ggcgatggcg
      781 cggctggtag atgacctgcg ccgggttgcc gggctcacgc tggacttcca gccgctcgac
      841 ggcctgggtg ctggcgaaac gctgggggcc tcgttgcgcc ttctgctcgc ccgtccggcg
      901 gtggaacgcc agcgctgaga tcc
//
