LOCUS       sequence-247597-       13563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13563)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 13563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX179
FEATURES             Location/Qualifiers
     source          1..13563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2911..2935
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3677..3701)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3730..3819
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      3853..4105
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(4534..4786)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5254..6279)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(5254..6279)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6289..6633)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(6775..6792)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6775..6791)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6784..6806)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6931..6950)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7141..7162
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    7409..7433
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(9029..10177)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10476..11270)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  11462..12229
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      12922..13553
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag aacacggggg acaagtttgt acaaaaaagc aggctccacc
     2941 actagtatgg acttcgaaaa tcatcaagaa gaagaagaaa ctgaactacc accgagacac
     3001 gacgactcac tcgataactc gaacaaaact cacccaacaa aaatgccgag tcctacacca
     3061 atggaactcg aacccttagc ggtggttcac acgtggcata ataatagacc aaagtacaaa
     3121 gagtgtctga agaaccacgc cgtaactatc ggcggccacg ccgtagacgg ctgtggtgag
     3181 tttatgcctg ccggtgaaga tggctcactt gattccctta aatgtgctgc ctgtaactgc
     3241 caccgcaatt tccaccgtaa aattactcaa ccaccaccca acgccattga atcgctgccg
     3301 tttgttaatt accattcgcc gtactacaga agcttaccgc caccttgtgg ttatccacag
     3361 taccacgtgg cgccacaaca aagaccgtta gctttgccgt cgacttctgg aggtggtgtc
     3421 taccgtgaag atcaggagga catgtccaac ctcaataata gcggaggcag tggttcaaag
     3481 aagcgatttc ggacaaaatt tagccaagaa cagaaggaga aaatgcaaga attagcagat
     3541 aaattggggt ggcggattca aagggaagat gaggagttgg tgcagcaatt gtgcaacgaa
     3601 actggggtga gaaggcaagt gtttaaagtt tggatgcata acaacaagaa cactcttggt
     3661 aagaaacccc ccggggaccc agctttcttg tacaaagtgg ttgataacag cgggttaatt
     3721 aacatctttt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     3781 tatgcaggat cctatccata tgacgttcca gattacgctg ctcagtgcag cgcttagagc
     3841 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     3901 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     3961 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     4021 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     4081 gcggtgtcat ctatgttact agatcgggaa ttagcttcat caacgcaaga catgcgcacg
     4141 accgtctgac aggagaggaa tttccgacga gcacagaaag gacttgctct tggacgtagg
     4201 cctatttctc aggcacatgt atcaagtgtt cggacgtggg ttttcgatgg tgtatcagcc
     4261 gccgccaact gggagatgag gaggctttct tggggggcag tcagcagttc atttcacaag
     4321 acagaggaac ttgtaaggag atgcactgat ttatcttggc gcaaaccagc aggacgaatt
     4381 agtgggaata gcccgcgaat atctaagtta tgcctgtcgg catgagcaga aacttccaat
     4441 tcgaaacagt ttggagaggt tgtttttggg catacctttt gttagtcagc ctctcgattg
     4501 ctcatcgtca ttacacagta ccgaagtttg atcgatctag taacatagat gacaccgcgc
     4561 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     4621 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     4681 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     4741 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatctgct tcgacgcact
     4801 ccttctttac tccaccatct cgtccttatt gaaaacgtgg gtagcaccaa aacgaatcaa
     4861 gtcgctggaa ctgaagttac caatcacgct ggatgatttg ccagttggat taatcttgcc
     4921 tttccccgca tgaataatat tgatgaatgc atgcgtgagg ggtatttcga ttttggcaat
     4981 agctgcaatt gccgcgacat cctccaacga gcataattct tcagaaaaat agcgatgttc
     5041 catgttgtca gggcatgcat gatgcacgtt atgaggtgac ggtgctaggc agtattccct
     5101 caaagtttca tagtcagtat catattcatc attgcattcc tgcaagagag aattgagacg
     5161 caatccacac gctgcggcaa ccttccggcg ttcgtggtct atttgctctt ggacgttgca
     5221 aacgtaagtg ttggatcccg gtcggcatct actctattcc tttgccctcg gacgagtgct
     5281 ggggcgtcgg tttccactat cggcgagtac ttctacacag ccatcggtcc agacggccgc
     5341 gcttctgcgg gcgatttgtg tacgcccgac agtcccggct ccggatcgga cgattgcgtc
     5401 gcatcgaccc tgcgcccaag ctgcatcatc gaaattgccg tcaaccaagc tctgatagag
     5461 ttggtcaaga ccaatgcgga gcatatacgc ccggagccgc ggcgatcctg caagctccgg
     5521 atgcctccgc tcgaagtagc gcgtctgctg ctccatacaa gccaaccacg gcctccagaa
     5581 gaagatgttg gcgacctcgt attgggaatc cccgaacatc gcctcgctcc agtcaatgac
     5641 cgctgttatg cggccattgt ccgtcaggac attgttggag ccgaaatccg cgtgcacgag
     5701 gtgccggact tcggggcagt cctcggccca aagcatcagc tcatcgagag cctgcgcgac
     5761 ggacgcactg acggtgtcgt ccatcacagt ttgccagtga tacacatggg gatcagcaat
     5821 cgcgcatatg aaatcacgcc atgtagtgta ttgaccgatt ccttgcggtc cgaatgggcc
     5881 gaacccgctc gtctggctaa gatcggccgc agcgatcgca tccatggcct ccgcgaccgg
     5941 ctgcagaaca gcgggcagtt cggtttcagg caggtcttgc aacgtgacac cctgtgcacg
     6001 gcgggagatg caataggtca ggctctcgct gaattcccca atgtcaagca cttccggaat
     6061 cgggagcgcg gccgatgcaa agtgccgata aacataacga tctttgtaga aaccatcggc
     6121 gcagctattt acccgcagga catatccacg ccctcctaca tcgaagctga aagcacgaga
     6181 ttcttcgccc tccgagagct gcatcaggtc ggagacgctg tcgaactttt cgatcagaaa
     6241 cttctcgaca gacgtcgcgg tgagttcagg ctttttcata tcggggtcgt cctctccaaa
     6301 tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga ttgtgcgtca
     6361 tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg
     6421 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
     6481 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
     6541 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
     6601 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
     6661 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttgac ggatctctag
     6721 gacgcgtcga cgcgtcgacg cgtcgacgcg tcgacgcgtc ctagaagcta attcactggc
     6781 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     6841 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     6901 ccaacagttg cgcagcctga atggcgcccg ctcctttcgc tttcttccct tcctttctcg
     6961 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     7021 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     7081 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     7141 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     7201 tataagggat tttgccgatt tcggaaccac catcaaacag gattttcgcc tgctggggca
     7261 aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg gcaatcagct
     7321 gttgcccgtc tcactggtga aaagaaaaac caccccagta cattaaaaac gtccgcaatg
     7381 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     7441 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     7501 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     7561 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     7621 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aaatatcatc
     7681 tccctcgcag agatccgaat tatcagcctt cttattcatt tctcgcttaa ccgtgacagg
     7741 ctgtcgatct tgagaactat gccgacataa taggaaatcg ctggataaag ccgctgagga
     7801 agctgagtgg cgctatttct ttagaagtga acgttgacga tatcaactcc cctatccatt
     7861 gctcaccgaa tggtacaggt cggggacccg aagttccgac tgtcggcctg atgcatcccc
     7921 ggctgatcga ccccagatct ggggctgaga aagcccagta aggaaacaac tgtaggttcg
     7981 agtcgcgaga tcccccggaa ccaaaggaag taggttaaac ccgctccgat caggccgagc
     8041 cacgccaggc cgagaacatt ggttcctgta ggcatcggga ttggcggatc aaacactaaa
     8101 gctactggaa cgagcagaag tcctccggcc gccagttgcc aggcggtaaa ggtgagcaga
     8161 ggcacgggag gttgccactt gcgggtcagc acggttccga acgccatgga aaccgccccc
     8221 gccaggcccg ctgcgacgcc gacaggatct agcgctgcgt ttggtgtcaa caccaacagc
     8281 gccacgcccg cagttccgca aatagccccc aggaccgcca tcaatcgtat cgggctacct
     8341 agcagagcgg cagagatgaa cacgaccatc agcggctgca cagcgcctac cgtcgccgcg
     8401 accccgcccg gcaggcggta gaccgaaata aacaacaagc tccagaatag cgaaatatta
     8461 agtgcgccga ggatgaagat gcgcatccac cagattcccg ttggaatctg tcggacgatc
     8521 atcacgagca ataaacccgc cggcaacgcc cgcagcagca taccggcgac ccctcggcct
     8581 cgctgttcgg gctccacgaa aacgccggac agatgcgcct tgtgagcgtc cttggggccg
     8641 tcctcctgtt tgaagaccga cagcccaatg atctcgccgt cgatgtaggc gccgaatgcc
     8701 acggcatctc gcaaccgttc agcgaacgcc tccatgggct ttttctcctc gtgctcgtaa
     8761 acggacccga acatctctgg agctttcttc agggccgaca atcggatctc gcggaaatcc
     8821 tgcacgtcgg ccgctccaag ccgtcgaatc tgagccttaa tcacaattgt caattttaat
     8881 cctctgttta tcggcagttc gtagagcgcg ccgtgcgtcc cgagcgatac tgagcgaagc
     8941 aagtgcgtcg agcagtgccc gcttgttcct gaaatgccag taaagcgctg gctgctgaac
     9001 ccccagccgg aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga
     9061 cccaggcgtg ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct
     9121 cgcgccactt cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct
     9181 tgagcgggta cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg
     9241 acagcttgcg gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga
     9301 cgatttcctc gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc
     9361 ggaagcggtg cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca
     9421 tcgccgtcgc ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga
     9481 tcgaccagcc caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag
     9541 gggtgcgctt cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca
     9601 gctcgacgcc ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt
     9661 gcagcgcctc gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt
     9721 cgtttggcat cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca
     9781 tttccttgat ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct
     9841 gttttgccag gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt
     9901 cgatggtcat cgacttcgcc aaacctgccg cctcctgttc gagacgacgc gaacgctcca
     9961 cggcggccga tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct
    10021 tggccgtagc ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca
    10081 tgacggtgcg gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt
    10141 cttgcctgta tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc
    10201 cgactcacgc cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt
    10261 ccagataatc caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct
    10321 cgtacttggt attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact
    10381 tgccgtgggc ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct
    10441 gcttgtcgcc ggcatcgttg cgccacatct aggtactaaa acaattcatc cagtaaaata
    10501 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
    10561 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
    10621 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
    10681 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
    10741 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
    10801 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
    10861 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
    10921 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
    10981 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
    11041 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
    11101 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
    11161 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
    11221 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
    11281 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
    11341 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
    11401 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccacaattat
    11461 gggtgatgct gccaacttac tgatttagtg tatgatggtg tttttgaggt gctccagtgg
    11521 cttctgtttc tatcagctgt ccctcctgtt cagctactga cggggtggtg cgtaacggca
    11581 aaagcaccgc cggacatcag cgctatctct gctctcactg ccgtaaaaca tggcaactgc
    11641 agttcactta caccgcttct caacccggta cgcaccagaa aatcattgat atggccatga
    11701 atggcgttgg atgccgggca acagcccgca ttatgggcgt tggcctcaac acgattttac
    11761 gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacagccgg gcagtgacgt
    11821 catcgtctgc gcggaaatgg acgaacagtg gggctatgtc ggggctaaat cgcgccagcg
    11881 ctggctgttt tacgcgtatg acagtctccg gaagacggtt gttgcgcacg tattcggtga
    11941 acgcactatg gcgacgctgg ggcgtcttat gagcctgctg tcaccctttg acgtggtgat
    12001 atggatgacg gatggctggc cgctgtatga atcccgcctg aagggaaagc tgcacgtaat
    12061 cagcaagcga tatacgcagc gaattgagcg gcataacctg aatctgaggc agcacctggc
    12121 acggctggga cggaagtcgc tgtcgttctc aaaatcggtg gagctgcatg acaaagtcat
    12181 cgggcattat ctgaacataa aacactatca ataagttgga gtcattaccc aattatgata
    12241 gaatttacaa gctataaggt tattgtcctg ggtttcaagc attagtccat gcaagttttt
    12301 atgctttgcc cattctatag atatattgat aagcgcgctg cctatgcctt gccccctgaa
    12361 atccttacat acggcgatat cttctatata aaagatatat tatcttatca gtattgtcaa
    12421 tatattcaag gcaatctgcc tcctcatcct cttcatcctc ttcgtcttgg tagcttttta
    12481 aatatggcgc ttcatagagt aattctgtaa aggtccaatt ctcgttttca tacctcggta
    12541 taatcttacc tatcacctca aatggttcgc tgggtttatc gcacccccga acacgagcac
    12601 ggcacccgcg accactatgc caagaatgcc caaggtaaaa attgccggcc ccgccatgaa
    12661 gtccgtgaat gccccgacgg ccgaagtgaa gggcaggccg ccacccaggc cgccgccctc
    12721 actgcccggc acctggtcgc tgaatgtcga tgccagcacc tgcggcacgt caatgcttcc
    12781 gggcgtcgcg ctcgggctga tcgcccatcc cgttactgcc ccgatcccgg caatggcaag
    12841 gactgccagc gctgccattt ttggggtgag gccgttcgcg gccgaggggc gcagcccctg
    12901 gggggatggg aggcccgcgt tagcgggccg ggagggttcg agaagggggg gcacccccct
    12961 tcggcgtgcg cggtcacgcg cacagggcgc agccctggtt aaaaacaagg tttataaata
    13021 ttggtttaaa agcaggttaa aagacaggtt agcggtggcc gaaaaacggg cggaaaccct
    13081 tgcaaatgct ggattttctg cctgtggaca gcccctcaaa tgtcaatagg tgcgcccctc
    13141 atctgtcagc actctgcccc tcaagtgtca aggatcgcgc ccctcatctg tcagtagtcg
    13201 cgcccctcaa gtgtcaatac cgcagggcac ttatccccag gcttgtccac atcatctgtg
    13261 ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga ggctggccag ctccacgtcg
    13321 ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc gccgggtgag tcggcccctc
    13381 aagtgtcaac gtccgcccct catctgtcag tgagggccaa gttttccgcg aggtatccac
    13441 aacgccggcg gccgcggtgt ctcgcacacg gcttcgacgg cgtttctggc gcgtttgcag
    13501 ggccatagac ggccgccagc ccagcggcga gggcaaccag cccggtgagc gtcggaaagg
    13561 cgc
//
