LOCUS       sequence-247602-       16936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16936)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 16936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX181
FEATURES             Location/Qualifiers
     source          1..16936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2584..2763
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        2616..2799
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             2820..3614
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2874..2893)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3484..3503
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4004..4256
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    4805..4826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4841..4846,4847..4864,4865..4871)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4879..4895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4884..4906
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4903..4919
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4903..4919
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        5443..5788
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    5810..5834
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7050..7074)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7103..7192
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      7226..7478
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(7907..8159)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8627..9652)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(8627..9652)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(9662..10006)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(10148..10165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10148..10164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10157..10179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(10304..10323)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10514..10535
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    10782..10806
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(12402..13550)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(13849..14643)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  14835..15602
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      16295..16926
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaactg aaggcgggaa acgacaatct gatcatgagc ggagaattaa
     2521 gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg tttggaactg
     2581 acagaaccgc aacgttgaag gagccactca gccgcgggtt tctggagttt aatgagctaa
     2641 gcacatacgt cagaaaccat tattgcgcgt tcaaaagtcg cctaaggtca ctatcagcta
     2701 gcaaatattt cttgtcaaaa atgctccact gacgttccat aaattcccct cggtatccaa
     2761 ttagagtctc atattcactc tcaatccaaa taatctgcac cggatctgga tcgtttcgca
     2821 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     2881 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     2941 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     3001 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     3061 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     3121 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     3181 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     3241 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     3301 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     3361 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     3421 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     3481 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     3541 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     3601 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct
     3661 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt
     3721 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc
     3781 ccacgggatc tctgcggaac aggcggtcga aggtgccgat atcattacga cagcaacggc
     3841 cgacaagcac aacgccacga tcctgagcga caatatgatc gggcccggcg tccacatcaa
     3901 cggcgtcggc ggcgactgcc caggcaagac cgagatgcac cgcgatatct tgctgcgttc
     3961 ggatattttc gtggagttcc cgccacagac ccggatgatc cccgatcgtt caaacatttg
     4021 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt
     4081 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag
     4141 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat
     4201 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgggc
     4261 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg
     4321 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct
     4381 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg
     4441 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta
     4501 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta
     4561 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg
     4621 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg
     4681 aatgtcgccc ttttgtcttt ggcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4741 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4801 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4861 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4921 atgattacgc caagcttgca tgcctgcagg tccccagatt agccttttca atttcagaaa
     4981 gaatgctaac ccacagatgg ttagagaggc ttacgcagca ggtctcatca agacgatcta
     5041 cccgagcaat aatctccagg aaatcaaata ccttcccaag aaggttaaag atgcagtcaa
     5101 aagattcagg actaactgca tcaagaacac agagaaagat atatttctca agatcagaag
     5161 tactattcca gtatggacga ttcaaggctt gcttcacaaa ccaaggcaag taatagagat
     5221 tggagtctct aaaaaggtag ttcccactga atcaaaggcc atggagtcaa agattcaaat
     5281 agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg
     5341 actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc
     5401 caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag
     5461 ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa
     5521 gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat
     5581 cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
     5641 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
     5701 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
     5761 aggaagttca tttcatttgg agagaacacg ggggactcta gagttatcaa caagtttgta
     5821 caaaaaagca ggctccacca tgctaactat atcacctttg agctacaaaa ccaccaaaaa
     5881 tgacagggat aatcacatgg aaagtttcac gatgcgagga gttgatgatt ttccagagtt
     5941 tggtgatggg aatttgctcg aaagcattga ttttgatgac attttcttgg ggattaacgt
     6001 cgacgatggt gttctaccag atttggagat ggactcagag attcttgctg aattatcagt
     6061 tagcagtggc gacgaatctg atcgtatgaa tagttataat actactacac caccactcca
     6121 agtagaagaa cacaactctt ctacaaaaga agaagtggaa aaagtagtat cacatttgaa
     6181 taagaagagt gaaaaaacta aacctaagtc aaaggagaag tccaagaatc cagaagggaa
     6241 gagaaaagtg aaggtggatt ggacgccaga gctacatcga agatttgtgc aagcagtaga
     6301 gcaattaggt gtagataaag cagtcccatc taggatttta gaacttatgg gtgttgaagg
     6361 tctcactcgc cataacatag ctagccatct tcaaaaatat agagcccatc ggaaacattt
     6421 gcttgcgaga gaagcggagg cggcaagttg gagccaaaga aaacaaatgt acggcagtgc
     6481 agccgtggtt ggaggtggcg gaaagagaga cataaaccca tggccgtcag caccaaccat
     6541 gggttttcca cctatgacgg caccacccat ggtgccgcct cattttagac ctctacatgt
     6601 ctggggtcat ccgcccaccg atcaatctat gatgcatatg tggccgaaac atatgatgcc
     6661 tctcccacca gcatgggcac cgattgtttc tcctcattct tcaccactta cagatcatcc
     6721 ttccttttgg cattcacacc atcaaagagt actaaactct cttgcaccag gcaccccttg
     6781 ctttccttca ccagtagcgc ccccgagatt tccagttcag ggcatcccac cccctgccat
     6841 ggtcaaagct gttcccaccg gagcacgaca agatctgcca aaacctcctt ctgattttca
     6901 tccttcaaag gagagcatag atgcggccat tggagatgtt ttagcaaagc catgtctacc
     6961 accaccaccc ctcggattga aacctccctc aatcgacagt gtgttgaatg aattacaacg
     7021 tcaagggatt aataaaatac ctccaactga cccagctttc ttgtacaaag tggttgataa
     7081 cagcgggtta attaacatct tttacccata cgatgttcct gactatgcgg gctatcccta
     7141 tgacgtcccg gactatgcag gatcctatcc atatgacgtt ccagattacg ctgctcagtg
     7201 cagcgcttag agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag
     7261 attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa
     7321 gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag
     7381 agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga
     7441 taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattagctt catcaacgca
     7501 agacatgcgc acgaccgtct gacaggagag gaatttccga cgagcacaga aaggacttgc
     7561 tcttggacgt aggcctattt ctcaggcaca tgtatcaagt gttcggacgt gggttttcga
     7621 tggtgtatca gccgccgcca actgggagat gaggaggctt tcttgggggg cagtcagcag
     7681 ttcatttcac aagacagagg aacttgtaag gagatgcact gatttatctt ggcgcaaacc
     7741 agcaggacga attagtggga atagcccgcg aatatctaag ttatgcctgt cggcatgagc
     7801 agaaacttcc aattcgaaac agtttggaga ggttgttttt gggcatacct tttgttagtc
     7861 agcctctcga ttgctcatcg tcattacaca gtaccgaagt ttgatcgatc tagtaacata
     7921 gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     7981 tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     8041 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     8101 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     8161 gcttcgacgc actccttctt tactccacca tctcgtcctt attgaaaacg tgggtagcac
     8221 caaaacgaat caagtcgctg gaactgaagt taccaatcac gctggatgat ttgccagttg
     8281 gattaatctt gcctttcccc gcatgaataa tattgatgaa tgcatgcgtg aggggtattt
     8341 cgattttggc aatagctgca attgccgcga catcctccaa cgagcataat tcttcagaaa
     8401 aatagcgatg ttccatgttg tcagggcatg catgatgcac gttatgaggt gacggtgcta
     8461 ggcagtattc cctcaaagtt tcatagtcag tatcatattc atcattgcat tcctgcaaga
     8521 gagaattgag acgcaatcca cacgctgcgg caaccttccg gcgttcgtgg tctatttgct
     8581 cttggacgtt gcaaacgtaa gtgttggatc ccggtcggca tctactctat tcctttgccc
     8641 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
     8701 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
     8761 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
     8821 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
     8881 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
     8941 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
     9001 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
     9061 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
     9121 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
     9181 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
     9241 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
     9301 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     9361 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     9421 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     9481 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     9541 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     9601 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttc atatcggggt
     9661 cgtcctctcc aaatgaaatg aacttcctta tatagaggaa gggtcttgcg aaggatagtg
     9721 ggattgtgcg tcatccctta cgtcagtgga gatatcacat caatccactt gctttgaaga
     9781 cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc catctttggg
     9841 accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat gatggcattt
     9901 gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag ctgggcaatg
     9961 gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag ccctttggtc
    10021 ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct ccaccatgtt
    10081 gacggatctc taggacgcgt cgacgcgtcg acgcgtcgac gcgtcgacgc gtcctagaag
    10141 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
    10201 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
    10261 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt cgctttcttc
    10321 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    10381 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
    10441 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    10501 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
    10561 tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa caggattttc
    10621 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
    10681 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca gtacattaaa
    10741 aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc
    10801 ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat
    10861 acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact
    10921 ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac
    10981 ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg
    11041 atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc atttctcgct
    11101 taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa tcgctggata
    11161 aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga cgatatcaac
    11221 tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc gactgtcggc
    11281 ctgatgcatc cccggctgat cgaccccaga tctggggctg agaaagccca gtaaggaaac
    11341 aactgtaggt tcgagtcgcg agatcccccg gaaccaaagg aagtaggtta aacccgctcc
    11401 gatcaggccg agccacgcca ggccgagaac attggttcct gtaggcatcg ggattggcgg
    11461 atcaaacact aaagctactg gaacgagcag aagtcctccg gccgccagtt gccaggcggt
    11521 aaaggtgagc agaggcacgg gaggttgcca cttgcgggtc agcacggttc cgaacgccat
    11581 ggaaaccgcc cccgccaggc ccgctgcgac gccgacagga tctagcgctg cgtttggtgt
    11641 caacaccaac agcgccacgc ccgcagttcc gcaaatagcc cccaggaccg ccatcaatcg
    11701 tatcgggcta cctagcagag cggcagagat gaacacgacc atcagcggct gcacagcgcc
    11761 taccgtcgcc gcgaccccgc ccggcaggcg gtagaccgaa ataaacaaca agctccagaa
    11821 tagcgaaata ttaagtgcgc cgaggatgaa gatgcgcatc caccagattc ccgttggaat
    11881 ctgtcggacg atcatcacga gcaataaacc cgccggcaac gcccgcagca gcataccggc
    11941 gacccctcgg cctcgctgtt cgggctccac gaaaacgccg gacagatgcg ccttgtgagc
    12001 gtccttgggg ccgtcctcct gtttgaagac cgacagccca atgatctcgc cgtcgatgta
    12061 ggcgccgaat gccacggcat ctcgcaaccg ttcagcgaac gcctccatgg gctttttctc
    12121 ctcgtgctcg taaacggacc cgaacatctc tggagctttc ttcagggccg acaatcggat
    12181 ctcgcggaaa tcctgcacgt cggccgctcc aagccgtcga atctgagcct taatcacaat
    12241 tgtcaatttt aatcctctgt ttatcggcag ttcgtagagc gcgccgtgcg tcccgagcga
    12301 tactgagcga agcaagtgcg tcgagcagtg cccgcttgtt cctgaaatgc cagtaaagcg
    12361 ctggctgctg aacccccagc cggaactgac cccacaaggc cctagcgttt gcaatgcacc
    12421 aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc ttcgcaggct
    12481 tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca catgaggcgg
    12541 aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc gaacatccgt
    12601 cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta gtggtcgcca
    12661 gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt tttcttgcca
    12721 cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc aacgcggtcg
    12781 gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc cttcgtgtaa
    12841 taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt gaaggtgatc
    12901 ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac cagttcgtca
    12961 tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt gacgtggaaa
    13021 atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt gaacagggca
    13081 gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc aatatcgaac
    13141 aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc ggcctgcttg
    13201 gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt ggtcgtcata
    13261 gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg ttcgagacga
    13321 cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag ttgcacgctg
    13381 tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg gaaggtttcg
    13441 cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc ggaaaacccc
    13501 gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat tcaccctcct
    13561 tgcgggattg ccccgactca cgccggggca atgtgccctt attcctgatt tgacccgcct
    13621 ggtgccttgg tgtccagata atccacctta tcggcaatga agtcggtccc gtagaccgtc
    13681 tggccgtcct tctcgtactt ggtattccga atcttgccct gcacgaatac cagcgacccc
    13741 ttgcccaaat acttgccgtg ggcctcggcc tgagagccaa aacacttgat gcggaagaag
    13801 tcggtgcgct cctgcttgtc gccggcatcg ttgcgccaca tctaggtact aaaacaattc
    13861 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
    13921 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
    13981 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
    14041 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
    14101 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
    14161 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
    14221 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
    14281 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
    14341 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
    14401 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
    14461 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
    14521 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
    14581 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
    14641 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
    14701 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
    14761 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
    14821 aaaccacaat tatgggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
    14881 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
    14941 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa
    15001 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt
    15061 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc
    15121 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
    15181 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
    15241 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
    15301 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
    15361 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
    15421 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
    15481 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
    15541 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
    15601 cccaattatg atagaattta caagctataa ggttattgtc ctgggtttca agcattagtc
    15661 catgcaagtt tttatgcttt gcccattcta tagatatatt gataagcgcg ctgcctatgc
    15721 cttgccccct gaaatcctta catacggcga tatcttctat ataaaagata tattatctta
    15781 tcagtattgt caatatattc aaggcaatct gcctcctcat cctcttcatc ctcttcgtct
    15841 tggtagcttt ttaaatatgg cgcttcatag agtaattctg taaaggtcca attctcgttt
    15901 tcatacctcg gtataatctt acctatcacc tcaaatggtt cgctgggttt atcgcacccc
    15961 cgaacacgag cacggcaccc gcgaccacta tgccaagaat gcccaaggta aaaattgccg
    16021 gccccgccat gaagtccgtg aatgccccga cggccgaagt gaagggcagg ccgccaccca
    16081 ggccgccgcc ctcactgccc ggcacctggt cgctgaatgt cgatgccagc acctgcggca
    16141 cgtcaatgct tccgggcgtc gcgctcgggc tgatcgccca tcccgttact gccccgatcc
    16201 cggcaatggc aaggactgcc agcgctgcca tttttggggt gaggccgttc gcggccgagg
    16261 ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt tcgagaaggg
    16321 ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg gttaaaaaca
    16381 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
    16441 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
    16501 aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg cgcccctcat
    16561 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
    16621 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
    16681 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
    16741 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
    16801 gcgaggtatc cacaacgccg gcggccgcgg tgtctcgcac acggcttcga cggcgtttct
    16861 ggcgcgtttg cagggccata gacggccgcc agcccagcgg cgagggcaac cagcccggtg
    16921 agcgtcggaa aggcgc
//
