LOCUS       sequence-247608-       15329 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  With a Nicotiana benthamiana transcription factor in PGWB14 
            backbone, for Agrobacterium mediated transient or stable expression 
            corresponding transcription factor fused with a C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15329)
  TITLE     Plant transcription factors for Agrobacterium-mediated transient 
            expression or plant transformation.
REFERENCE   2  (bases 1 to 15329)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGWB14-LYX186
FEATURES             Location/Qualifiers
     source          1..15329
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(127..498)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            531..640
                     /note="incP origin of transfer"
     primer_bind     863..882
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1116..1133
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1321..1971)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline resistance regulatory protein"
                     /label=TetR
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     misc_feature    2436..2460
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        2559..2904
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    3531..3707
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4210..4555
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    4577..4601
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5475..5499)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5528..5617
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     terminator      5651..5903
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     terminator      complement(6332..6584)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(7052..8077)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(7052..8077)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(8087..8431)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(8541..8558)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8541..8557)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8550..8572)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8697..8716)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8907..8928
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    9175..9199
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(10795..11943)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(12242..13036)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLLLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     mobile_element  13228..13995
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     rep_origin      14688..15319
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 tcggtcttgc cttgctcgtc ggtgatgtac ttcaccagct ccgcgaagtc gctcttcttg
       61 atggagcgca tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta
      121 aaaaagtcat ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcgtc
      181 ctgcttctct tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt
      241 gcgcgggtcg tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt
      301 gatgcgggcc agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg
      361 gccgacggcc agcaggtagg ccgacaggct catgccggcc gccgccgcct tttcctcaat
      421 cgctcttcgt tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg
      481 cttggtttca tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc
      541 tcgcagagca ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga
      601 acacccgctc gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca
      661 ccaaggaaag tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg
      721 atataccgaa aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagcgcca
      781 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      841 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      901 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      961 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     1021 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     1081 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     1141 aagagcgcca gaaggccgcc agagaggccg agcgcggccg tgaggcttgg acgctagggc
     1201 agggcatgaa aaagcccgta gcgggctgct acgggcgtct gacgcggtgg aaagggggag
     1261 gggatgttgt ctacatggct ctgctgtagt gagtgggttg cgctccggca gcggtcctga
     1321 tcaatcgtca ccctttctcg gtccttcaac gttcctgaca acgagcctcc ttttcgccaa
     1381 tccatcgaca atcaccgcga gtccctgctc gaacgctgcg tccggaccgg cttcgtcgaa
     1441 ggcgtctatc gcggcccgca acagcggcga gagcggagcc tgttcaacgg tgccgccgcg
     1501 ctcgccggca tcgctgtcgc cggcctgctc ctcaagcacg gccccaacag tgaagtagct
     1561 gattgtcatc agcgcattga cggcgtcccc ggccgaaaaa cccgcctcgc agaggaagcg
     1621 aagctgcgcg tcggccgttt ccatctgcgg tgcgcccggt cgcgtgccgg catggatgcg
     1681 cgcgccatcg cggtaggcga gcagcgcctg cctgaagctg cgggcattcc cgatcagaaa
     1741 tgagcgccag tcgtcgtcgg ctctcggcac cgaatgcgta tgattctccg ccagcatggc
     1801 ttcggccagt gcgtcgagca gcgcccgctt gttcctgaag tgccagtaaa gcgccggctg
     1861 ctgaaccccc aaccgttccg ccagtttgcg tgtcgtcaga ccgtctacgc cgacctcgtt
     1921 caacaggtcc agggcggcac ggatcactgt attcggctgc aactttgtca tgcttgacac
     1981 tttatcactg ataaacataa tatgtccacc aacttatcag tgataaagaa tccgcgcgtt
     2041 caatcggacc agcggaggct ggtccggagg ccagacgtga aacccaacat acccctgatc
     2101 gtaattctga gcactgtcgc gctcgacgct gtcggcatcg gcctgattat gccggtgctg
     2161 ccgggcctcc tgcgcgatct ggttcactcg aacgacgtca ccgcccacta tggcattctg
     2221 ctggcgctgt atgcgttggt gcaatttgcc tgcgcacctg tgctgggcgc gctgtcggat
     2281 cgtttcgggc ggcggccaat cttgctcgtc tcgctggccg gcgccagatc tggggaaccc
     2341 tgtggttggc atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat
     2401 atccgattat tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca
     2461 aacactgata gtttaaacgt tgcgattaag aattcgagct cggtaccccc ctactccaaa
     2521 aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca acaaagggta
     2581 atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat cgaaaggaca
     2641 gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggctatcatt
     2701 caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg
     2761 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga catctccact
     2821 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
     2881 agttcatttc atttggagag gacagcccaa gcttcgactc tagaggatcc ccttaaatcg
     2941 atatggaacg agctatacaa ggaaacgacg ctagggaaca agctaacagt gaacgttggg
     3001 atggaggatc aggaggtacc acttctccct tcaaacttcc tgacgaaagt ccgagttgga
     3061 ctgagtggcg gctacataac gatgagacga attcgaatca agataatccc cttggtttca
     3121 aggaaagctg gggtttcggg aaagttgtat ttaagagata tctcagatac gacaggacgg
     3181 aagcttcact gcacagagtc cttggatctt ggacgggaga ttcggttaac tatgcagcat
     3241 ctcgattttt cggtttcgac cagatcggat gtacctatag tattcggttt cgaggagtta
     3301 gtatcaccgt ttctggaggg tctcgaactc ttcagcatct ctgtgagatg gcaattcggt
     3361 ctaagcaaga actgctacag cttgccccaa tcgaagtgga aagtaatgta tcaagaggat
     3421 gccctgaagg tactcaaacc ttcgaaaaag aaagcgagta agtcgagggc gagctcgaat
     3481 tcggtacgct gaaatcacca gtctctctct acaaatctat ctctctctat tttctccata
     3541 aataatgtgt gagtagtttc ccgataaggg aaattagggt tcttataggg tttcgctcat
     3601 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
     3661 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatctcc ctgcaggtcc
     3721 ccagattagc cttttcaatt tcagaaagaa tgctaaccca cagatggtta gagaggctta
     3781 cgcagcaggt ctcatcaaga cgatctaccc gagcaataat ctccaggaaa tcaaatacct
     3841 tcccaagaag gttaaagatg cagtcaaaag attcaggact aactgcatca agaacacaga
     3901 gaaagatata tttctcaaga tcagaagtac tattccagta tggacgattc aaggcttgct
     3961 tcacaaacca aggcaagtaa tagagattgg agtctctaaa aaggtagttc ccactgaatc
     4021 aaaggccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
     4081 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
     4141 ggtggagcac gacacacttg tctactccaa aaatatcaaa gatacagtct cagaagacca
     4201 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
     4261 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
     4321 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
     4381 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     4441 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     4501 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga gaacacgggg
     4561 gactctagag ttatcaacaa gtttgtacaa aaaagcaggc tccaccacta gtatggaccc
     4621 ttattcctct ggagaggaac ttgttgttaa gacaaggaag ccttatacca tcactaagca
     4681 acgagagcga tggacagagg aggagcacaa taggttccta gaagccttga aactctatgg
     4741 gcgtgcttgg caacgcatag aagaacacat aggaacaaaa actgccgtgc aaatcagaag
     4801 tcatgcgcaa aaattcttta caaagttaga aaaggaggct gttataaagg gagtcccaat
     4861 aagtcaagca ctcaacattg aaattcctcc tccacgacct aaaaggaaac caagtaatcc
     4921 ttatcctcgt aagaccagcg tagcagttcc ctcatcacag gtgggaataa aggatggaaa
     4981 attatcaaca cctttttctt cgatatgtga ggataggaac ttatttgacc tggagaaaga
     5041 accgattgct gagaaacctg gtagaaacga gaagctgggc ggtgtacaag aagaccagag
     5101 caagaaaaat tgctcccaag ggttatctct tttcaaagaa ggtgcttctg ccccctctct
     5161 gtccctggga aagtctttgc aaacacttgc agaacctgca ggatcatgca ctttaaatga
     5221 gtctgtgcct gccgctaaag gagtgattaa ccatgatgta acagctaaat cttttcttac
     5281 tattgaatcc aaagagcatc aaaagttgga tatacttgac gctaaacagt cctttcaaag
     5341 taacagctct tgtaacacct tcaacggggg gaaatcttgt caatcgaatg agaagttggc
     5401 aagttcaagc tgtcaggacg aggaaaaaag cgccaagaga cttcgtttgg aaggagaagc
     5461 ttctattccc ggggacccag ctttcttgta caaagtggtt gataacagcg ggttaattaa
     5521 catcttttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     5581 tgcaggatcc tatccatatg acgttccaga ttacgctgct cagtgcagcg cttagagctc
     5641 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     5701 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     5761 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     5821 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     5881 ggtgtcatct atgttactag atcgggaatt agcttcatca acgcaagaca tgcgcacgac
     5941 cgtctgacag gagaggaatt tccgacgagc acagaaagga cttgctcttg gacgtaggcc
     6001 tatttctcag gcacatgtat caagtgttcg gacgtgggtt ttcgatggtg tatcagccgc
     6061 cgccaactgg gagatgagga ggctttcttg gggggcagtc agcagttcat ttcacaagac
     6121 agaggaactt gtaaggagat gcactgattt atcttggcgc aaaccagcag gacgaattag
     6181 tgggaatagc ccgcgaatat ctaagttatg cctgtcggca tgagcagaaa cttccaattc
     6241 gaaacagttt ggagaggttg tttttgggca taccttttgt tagtcagcct ctcgattgct
     6301 catcgtcatt acacagtacc gaagtttgat cgatctagta acatagatga caccgcgcgc
     6361 gataatttat cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat
     6421 tgcgggactc taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt
     6481 attacatgct taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca
     6541 ggattcaatc ttaagaaact ttattgccaa atgtttgaac gatctgcttc gacgcactcc
     6601 ttctttactc caccatctcg tccttattga aaacgtgggt agcaccaaaa cgaatcaagt
     6661 cgctggaact gaagttacca atcacgctgg atgatttgcc agttggatta atcttgcctt
     6721 tccccgcatg aataatattg atgaatgcat gcgtgagggg tatttcgatt ttggcaatag
     6781 ctgcaattgc cgcgacatcc tccaacgagc ataattcttc agaaaaatag cgatgttcca
     6841 tgttgtcagg gcatgcatga tgcacgttat gaggtgacgg tgctaggcag tattccctca
     6901 aagtttcata gtcagtatca tattcatcat tgcattcctg caagagagaa ttgagacgca
     6961 atccacacgc tgcggcaacc ttccggcgtt cgtggtctat ttgctcttgg acgttgcaaa
     7021 cgtaagtgtt ggatcccggt cggcatctac tctattcctt tgccctcgga cgagtgctgg
     7081 ggcgtcggtt tccactatcg gcgagtactt ctacacagcc atcggtccag acggccgcgc
     7141 ttctgcgggc gatttgtgta cgcccgacag tcccggctcc ggatcggacg attgcgtcgc
     7201 atcgaccctg cgcccaagct gcatcatcga aattgccgtc aaccaagctc tgatagagtt
     7261 ggtcaagacc aatgcggagc atatacgccc ggagccgcgg cgatcctgca agctccggat
     7321 gcctccgctc gaagtagcgc gtctgctgct ccatacaagc caaccacggc ctccagaaga
     7381 agatgttggc gacctcgtat tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg
     7441 ctgttatgcg gccattgtcc gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt
     7501 gccggacttc ggggcagtcc tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg
     7561 acgcactgac ggtgtcgtcc atcacagttt gccagtgata cacatgggga tcagcaatcg
     7621 cgcatatgaa atcacgccat gtagtgtatt gaccgattcc ttgcggtccg aatgggccga
     7681 acccgctcgt ctggctaaga tcggccgcag cgatcgcatc catggcctcc gcgaccggct
     7741 gcagaacagc gggcagttcg gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc
     7801 gggagatgca ataggtcagg ctctcgctga attccccaat gtcaagcact tccggaatcg
     7861 ggagcgcggc cgatgcaaag tgccgataaa cataacgatc tttgtagaaa ccatcggcgc
     7921 agctatttac ccgcaggaca tatccacgcc ctcctacatc gaagctgaaa gcacgagatt
     7981 cttcgccctc cgagagctgc atcaggtcgg agacgctgtc gaacttttcg atcagaaact
     8041 tctcgacaga cgtcgcggtg agttcaggct ttttcatatc ggggtcgtcc tctccaaatg
     8101 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     8161 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     8221 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     8281 ggcatcttga acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc
     8341 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     8401 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     8461 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttgacgg atctctagga
     8521 cgcgtcctag aagctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8581 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8641 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgcccgctcc
     8701 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8761 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8821 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8881 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8941 ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg aaccaccatc
     9001 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     9061 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     9121 ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac
     9181 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa
     9241 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt
     9301 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc
     9361 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc
     9421 gttgctgcct gtgatcaaat atcatctccc tcgcagagat ccgaattatc agccttctta
     9481 ttcatttctc gcttaaccgt gacaggctgt cgatcttgag aactatgccg acataatagg
     9541 aaatcgctgg ataaagccgc tgaggaagct gagtggcgct atttctttag aagtgaacgt
     9601 tgacgatatc aactccccta tccattgctc accgaatggt acaggtcggg gacccgaagt
     9661 tccgactgtc ggcctgatgc atccccggct gatcgacccc agatctgggg ctgagaaagc
     9721 ccagtaagga aacaactgta ggttcgagtc gcgagatccc ccggaaccaa aggaagtagg
     9781 ttaaacccgc tccgatcagg ccgagccacg ccaggccgag aacattggtt cctgtaggca
     9841 tcgggattgg cggatcaaac actaaagcta ctggaacgag cagaagtcct ccggccgcca
     9901 gttgccaggc ggtaaaggtg agcagaggca cgggaggttg ccacttgcgg gtcagcacgg
     9961 ttccgaacgc catggaaacc gcccccgcca ggcccgctgc gacgccgaca ggatctagcg
    10021 ctgcgtttgg tgtcaacacc aacagcgcca cgcccgcagt tccgcaaata gcccccagga
    10081 ccgccatcaa tcgtatcggg ctacctagca gagcggcaga gatgaacacg accatcagcg
    10141 gctgcacagc gcctaccgtc gccgcgaccc cgcccggcag gcggtagacc gaaataaaca
    10201 acaagctcca gaatagcgaa atattaagtg cgccgaggat gaagatgcgc atccaccaga
    10261 ttcccgttgg aatctgtcgg acgatcatca cgagcaataa acccgccggc aacgcccgca
    10321 gcagcatacc ggcgacccct cggcctcgct gttcgggctc cacgaaaacg ccggacagat
    10381 gcgccttgtg agcgtccttg gggccgtcct cctgtttgaa gaccgacagc ccaatgatct
    10441 cgccgtcgat gtaggcgccg aatgccacgg catctcgcaa ccgttcagcg aacgcctcca
    10501 tgggcttttt ctcctcgtgc tcgtaaacgg acccgaacat ctctggagct ttcttcaggg
    10561 ccgacaatcg gatctcgcgg aaatcctgca cgtcggccgc tccaagccgt cgaatctgag
    10621 ccttaatcac aattgtcaat tttaatcctc tgtttatcgg cagttcgtag agcgcgccgt
    10681 gcgtcccgag cgatactgag cgaagcaagt gcgtcgagca gtgcccgctt gttcctgaaa
    10741 tgccagtaaa gcgctggctg ctgaaccccc agccggaact gaccccacaa ggccctagcg
    10801 tttgcaatgc accaggtcat cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa
    10861 ctcttcgcag gcttcgccga cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc
    10921 gcacatgagg cggaaggttt ccagcttgag cgggtacggc tcccggtgcg agctgaaata
    10981 gtcgaacatc cgtcgggccg tcggcgacag cttgcggtac ttctcccata tgaatttcgt
    11041 gtagtggtcg ccagcaaaca gcacgacgat ttcctcgtcg atcaggacct ggcaacggga
    11101 cgttttcttg ccacggtcca ggacgcggaa gcggtgcagc agcgacaccg attccaggtg
    11161 cccaacgcgg tcggacgtga agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc
    11221 ggccttcgtg taataccggc cattgatcga ccagcccagg tcctggcaaa gctcgtagaa
    11281 cgtgaaggtg atcggctcgc cgataggggt gcgcttcgcg tactccaaca cctgctgcca
    11341 caccagttcg tcatcgtcgg cccgcagctc gacgccggtg taggtgatct tcacgtcctt
    11401 gttgacgtgg aaaatgacct tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt
    11461 ggtgaacagg gcagagcggg ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg
    11521 cgcaatatcg aacaaggaaa gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa
    11581 cgcggcctgc ttggcctcgc tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt
    11641 cttggtcgtc atagttcctc gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc
    11701 ctgttcgaga cgacgcgaac gctccacggc ggccgatggc gcgggcaggg cagggggagc
    11761 cagttgcacg ctgtcgcgct cgatcttggc cgtagcttgc tggaccatcg agccgacgga
    11821 ctggaaggtt tcgcggggcg cacgcatgac ggtgcggctt gcgatggttt cggcatcctc
    11881 ggcggaaaac cccgcgtcga tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt
    11941 cattcaccct ccttgcggga ttgccccgac tcacgccggg gcaatgtgcc cttattcctg
    12001 atttgacccg cctggtgcct tggtgtccag ataatccacc ttatcggcaa tgaagtcggt
    12061 cccgtagacc gtctggccgt ccttctcgta cttggtattc cgaatcttgc cctgcacgaa
    12121 taccagcgac cccttgccca aatacttgcc gtgggcctcg gcctgagagc caaaacactt
    12181 gatgcggaag aagtcggtgc gctcctgctt gtcgccggca tcgttgcgcc acatctaggt
    12241 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
    12301 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
    12361 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
    12421 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
    12481 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
    12541 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
    12601 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
    12661 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
    12721 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
    12781 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagca
    12841 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
    12901 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
    12961 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
    13021 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
    13081 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
    13141 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
    13201 ggagccgatt ttgaaaccac aattatgggt gatgctgcca acttactgat ttagtgtatg
    13261 atggtgtttt tgaggtgctc cagtggcttc tgtttctatc agctgtccct cctgttcagc
    13321 tactgacggg gtggtgcgta acggcaaaag caccgccgga catcagcgct atctctgctc
    13381 tcactgccgt aaaacatggc aactgcagtt cacttacacc gcttctcaac ccggtacgca
    13441 ccagaaaatc attgatatgg ccatgaatgg cgttggatgc cgggcaacag cccgcattat
    13501 gggcgttggc ctcaacacga ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc
    13561 tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg aaatggacga acagtggggc
    13621 tatgtcgggg ctaaatcgcg ccagcgctgg ctgttttacg cgtatgacag tctccggaag
    13681 acggttgttg cgcacgtatt cggtgaacgc actatggcga cgctggggcg tcttatgagc
    13741 ctgctgtcac cctttgacgt ggtgatatgg atgacggatg gctggccgct gtatgaatcc
    13801 cgcctgaagg gaaagctgca cgtaatcagc aagcgatata cgcagcgaat tgagcggcat
    13861 aacctgaatc tgaggcagca cctggcacgg ctgggacgga agtcgctgtc gttctcaaaa
    13921 tcggtggagc tgcatgacaa agtcatcggg cattatctga acataaaaca ctatcaataa
    13981 gttggagtca ttacccaatt atgatagaat ttacaagcta taaggttatt gtcctgggtt
    14041 tcaagcatta gtccatgcaa gtttttatgc tttgcccatt ctatagatat attgataagc
    14101 gcgctgccta tgccttgccc cctgaaatcc ttacatacgg cgatatcttc tatataaaag
    14161 atatattatc ttatcagtat tgtcaatata ttcaaggcaa tctgcctcct catcctcttc
    14221 atcctcttcg tcttggtagc tttttaaata tggcgcttca tagagtaatt ctgtaaaggt
    14281 ccaattctcg ttttcatacc tcggtataat cttacctatc acctcaaatg gttcgctggg
    14341 tttatcgcac ccccgaacac gagcacggca cccgcgacca ctatgccaag aatgcccaag
    14401 gtaaaaattg ccggccccgc catgaagtcc gtgaatgccc cgacggccga agtgaagggc
    14461 aggccgccac ccaggccgcc gccctcactg cccggcacct ggtcgctgaa tgtcgatgcc
    14521 agcacctgcg gcacgtcaat gcttccgggc gtcgcgctcg ggctgatcgc ccatcccgtt
    14581 actgccccga tcccggcaat ggcaaggact gccagcgctg ccatttttgg ggtgaggccg
    14641 ttcgcggccg aggggcgcag cccctggggg gatgggaggc ccgcgttagc gggccgggag
    14701 ggttcgagaa gggggggcac cccccttcgg cgtgcgcggt cacgcgcaca gggcgcagcc
    14761 ctggttaaaa acaaggttta taaatattgg tttaaaagca ggttaaaaga caggttagcg
    14821 gtggccgaaa aacgggcgga aacccttgca aatgctggat tttctgcctg tggacagccc
    14881 ctcaaatgtc aataggtgcg cccctcatct gtcagcactc tgcccctcaa gtgtcaagga
    14941 tcgcgcccct catctgtcag tagtcgcgcc cctcaagtgt caataccgca gggcacttat
    15001 ccccaggctt gtccacatca tctgtgggaa actcgcgtaa aatcaggcgt tttcgccgat
    15061 ttgcgaggct ggccagctcc acgtcgccgg ccgaaatcga gcctgcccct catctgtcaa
    15121 cgccgcgccg ggtgagtcgg cccctcaagt gtcaacgtcc gcccctcatc tgtcagtgag
    15181 ggccaagttt tccgcgaggt atccacaacg ccggcggccg cggtgtctcg cacacggctt
    15241 cgacggcgtt tctggcgcgt ttgcagggcc atagacggcc gccagcccag cggcgagggc
    15301 aaccagcccg gtgagcgtcg gaaaggcgc
//
