LOCUS       sequence-247615-        5946 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian DamID vector used to fuse gene of interest to V5-tagged 
            E.coli Dam. Can also express Dam alone as negative control..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Vogel MJ, Guelen L, de Wit E, Peric-Hupkes D, Loden M, Talhout W, 
            Feenstra M, Abbas B, Classen AK, van Steensel B
  TITLE     Human heterochromatin proteins form large domains containing 
            KRAB-ZNF genes.
  JOURNAL   Genome Res. 2006 Dec;16(12):1493-504. Epub 2006 Oct 12.
  PUBMED    17038565
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Res."; date: "2006-12"; volume: "16(12)"; pages: "1493-504. Epub 
            2006 Oct 12"
COMMENT     Sequence Label: pIND(V5)EcoDam
FEATURES             Location/Qualifiers
     source          1..5946
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        206..444
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     391..412
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             601..642
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1495..1512
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1535..1552)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1541..1765
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      1811..2239
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1898..1917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2108..2129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(2248..2268)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2253..2582
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2433..2568
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2495..2514
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             2649..3443
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2703..2722)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3313..3332
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3619..3740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3656..3675)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3710..3729
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3789..3805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3789..3805)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3802..3824)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3813..3829
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3837..3843,3844..3861,3862..3867))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3882..3903
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4020..4037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4191..4779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4271..4290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4950..5741,5742..5810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5573..5592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5811..5915)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctcgg ccgcatatta agtgcattgt tctcgatacc gctaagtgca
       61 ttgttctcgt tagctcgatg gacaagtgca ttgttctctt gctgaaagct cgatggacaa
      121 gtgcattgtt ctcttgctga aagctcgatg gacaagtgca ttgttctctt gctgaaagct
      181 cagtacccgg gagtaccctc gaccgccgga gtataaatag aggcgcttcg tctacggagc
      241 gacaattcaa ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa
      301 caagcgcagc tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt
      361 aaaagtaacc agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt
      421 attgaataca agaagagaac tctgaatact ttcaacaagt taccgagaaa gaagaactca
      481 cacacagcta gcgtttaaac ttaagcttgg taccgagctc ggatccacta gtccagtgtg
      541 gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg gcccttcgaa
      601 ggtaagccta tccctaaccc tctcctcggt ctcgattcta cgcgtaccgg catgaagaaa
      661 aatcgcgctt ttttgaagtg ggcagggggc aagtatcccc tgcttgatga tattaaacgg
      721 catttgccca agggcgaatg tctggttgag ccttttgtag gtgccgggtc ggtgtttctc
      781 aacaccgact tttctcgtta tatccttgcc gatatcaata gcgacctgat cagtctctat
      841 aacattgtga agatgcgtac tgatgagtac gtacaggccg cacgcgagct gtttgttccc
      901 gaaacaaatt gcgccgaggt ttactatcag ttccgcgaag agttcaacaa aagccaggat
      961 ccgttccgtc gggcggtact gtttttatat ttgaaccgct acggttacaa cggcctgtgt
     1021 cgttacaatc tgcgcggtga gtttaacgtg ccgttcggcc gctacaaaaa accctatttc
     1081 ccggaagcag agttgtatca cttcgctgaa aaagcgcaga atgccttttt ctattgtgag
     1141 tcttacgccg atagcatggc gcgcgcagat gatgcatccg tcgtctattg cgatccgcct
     1201 tatgcaccgc tgtctgcgac cgccaacttt acggcgtatc acacaaacag ttttacgctt
     1261 gaacaacaag cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg
     1321 ctgatctcca atcacgatac gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat
     1381 gtcgtcaaag ttcgacgcag tataagcagc aacggcggca cacgtaaaaa ggtggacgaa
     1441 ctgctggctt tgtacaaacc aggagtcgtt tcacccgcga aaaaataagc cggtcatcat
     1501 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     1561 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1621 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     1681 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     1741 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     1801 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1861 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1921 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1981 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     2041 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     2101 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     2161 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     2221 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
     2281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
     2341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     2401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     2461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     2521 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
     2581 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat
     2641 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     2701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     2761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     2821 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     2881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     2941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     3001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     3061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     3121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca
     3181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     3241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     3301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     3361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     3421 gccttcttga cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg
     3481 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     3541 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     3601 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3721 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     3781 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     3961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     4021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     4861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     4921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     4981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     5041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     5101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     5161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     5221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     5281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     5341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     5401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     5461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     5521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     5581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     5641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     5701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     5761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     5821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     5941 gacgtc
//
