LOCUS       sequence-24762-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pET16b-Mms2
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..85
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             98..109
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(577..595)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      591..638
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tgcgncattc cctctgaata ttttgtttac tttaagaagg agatatacca tgggccatca
       61 tcatcatcat catcatcatc atcacagcag cggccatatc gaaggtcgtc atatgtcaaa
      121 agtaccaaga aattttaggt tgttagaaga attagaaaag ggtgaaaaag ggtttggtcc
      181 agaatcatgt agttatggtt tagctgatag cgacgatatt accatgacca aatggaacgg
      241 cactatttta gggccccctc atagtaatca tgaaaacaga atctattccc tttctataga
      301 ttgtggccca aactatccag attctccccc aaaggtcact ttcatttcca agataaatct
      361 accatgcgtc aatccaacta cgggggaggt ccaaactgac ttccacacct tacgtgactg
      421 gaaaagagcc tacaccatgg aaactttatt acttgatctt agaaaggaaa tggcaacccc
      481 agcgaacaag aaattgcgtc aaccgaagga aggagaaacc ttttagggat ccggctgcta
      541 acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac
      601 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg
      661 gatatcccgc aagaggcccg gcagtacngn atanccagcc tatgcnnacn cntccaggtg
      721 acggtgccga ngangacaac aatgacngcn ttgttatatt ctacccgggg ctgntgcgta
      781 gcatttactg tgataactac gcattaagct acgatgatan tgnaactgaa atctgagnga
      841 aggctcngtc gcattttnag
//
