LOCUS       sequence-247620-        9699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tissue-specific multiplex gRNA plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9699)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 9699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD6
FEATURES             Location/Qualifiers
     source          1..9699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    147..241
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    265..359
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     misc_RNA        811..886
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    1594..2024
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    2058..2127
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(2346..2364)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2385..2401)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2385..2401)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2398..2420)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2409..2425
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2433..2439,2440..2457,2458..2463))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2478..2499
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2616..2633)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2787..3375)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2867..2886)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3546..4337,4338..4406))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4169..4188
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4407..4511)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4537..4992)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4674..4695)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4886..4905
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5119..5141
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5133..5150
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5134..5150
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5157..5176
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5157..5175
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5209..5227)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5679..5750,6152..6425,6500..7154,7216..7531,7735..7866,
                     7937..8551)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    9324..9357
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    join(9370..9699,1..101)
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
ORIGIN
        1 cccaataaaa gattattata ttgcatacct tttcttgcca taccatttag ccgatcaatt
       61 gtgctcggca acagtatatt tgtggtgtgc caaccaacaa cagatccaag ctggccgcgg
      121 ctcgatccgc ttgcatgcct gcaggtcgga gtactgtcct ccgagcggag tactgtcctc
      181 cgagcggagt actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga
      241 gcggaagctt gcatgcctgc aggtcggagt actgtcctcc gagcggagta ctgtcctccg
      301 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
      361 ggagactcta gcgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg
      421 ctgagagcat cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta
      481 atatcctttg aataagccaa ctttgaatca caagacgcat accaaacgaa ttcgggcttt
      541 gagtgtgtgt agacatcaag catcggtggt tcagtggtag aatgctcgcc tgccacgcgg
      601 gcggcccggg ttcgattccc ggccgatgca gggtcttcgt ttcagagcta tgctggaaac
      661 agcatagcaa gttgaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
      721 gtgctaacaa agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg
      781 ggttcgattc ccggctggtg cagaagacct gttttagagc tagaaatagc aagttaaaat
      841 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctaac aaagcaccag
      901 tggtctagtg gtagaatagt accctgccac ggtacagacc cgggttcgat tcccggctgg
      961 tgcatctaga ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     1021 ctacctacag agatttaaag ctctaattaa aataacaaat caattgtttt ataatattcg
     1081 tacgattctt tgattatgta ataaaatgtg atcattagga agattacgaa aaatataaaa
     1141 aatatgagtt ctgtgtgtat aacaaatgct gtaaacgcca caattgtgtt tgttgcaaat
     1201 aaacccatga ttatttgatt aaaattgttg ttttctttgt tcatagacaa tagtgtgttt
     1261 tgcctaaacg tgtactgcat aaactccatg cgagtgtata gcgagctagt ggctaacgct
     1321 tgccccacca aagtagattc gtcaaaatcc tcaatttcat caccctcctc caagtttaac
     1381 atttggccgt cggaattaac ttctaaagat gccacataat ctaataaatg aaatagagat
     1441 tcaaacgtgg cgtcatcgtc cgtttcgacc atttccgaaa agaactcggg cataaactct
     1501 atgatttctc tggacgtggt gttgtcgaaa ctctcaaagt acgcagtcag gaacgtgcgc
     1561 gacatgtcgt cgggaaactc gcgcggaaac atttggccac gtaataagtg tgcgttgaat
     1621 ttattcgcaa aaacattgca tattttcggc aaagtaaaat tttgttgcat accttatcaa
     1681 aaaataagtg ctgcatactt tttagagaaa ccaaataatt ttttattgca tacccgtttt
     1741 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacttt gacgaaacaa
     1801 attttcgttg catacccaat aaaagattat tatattgcat acccgttttt aataaaatac
     1861 attgcatacc ctcttttaat aaaaaatatt gcatacgttg acgaaacaaa ttttcgttgc
     1921 atacccaata aaagattatt atattgcata ccttttcttg ccataccatt tagccgatca
     1981 attgtgctcg gcaacagtat atttgtggtg tgccaaccaa caacggatcc actagtgtcg
     2041 acgatgtagg tcacagtctc gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc
     2101 ccgggcgcgt actccacctc acccatctgg tccatcatga tgaacgggtc gaggtggcgg
     2161 tagttgatcc cggcgaacgc gcggcgcacc gggaagccct cgccctcgaa accgctgggc
     2221 gcggtggtca cggtgagcac gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc
     2281 agcgtcagcg ggttctcgac ggtcacggcg ggcatgtcga cactagttct agccagcttt
     2341 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     2401 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2461 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     2521 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2581 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     2641 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     2701 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     2761 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     2821 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2881 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2941 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3001 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3061 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3121 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3181 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     3241 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3301 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3361 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3421 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3481 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     3541 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     3601 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     3661 ggccccagtg ctgcaatgat accgcgggac ccacgctcac cggctccaga tttatcagca
     3721 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     3781 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3841 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3901 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3961 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4021 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4081 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4141 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4201 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4261 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4321 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4381 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4441 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4501 ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt
     4561 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     4621 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     4681 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     4741 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     4801 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     4861 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     4921 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     4981 tacagggcgc gtcccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     5041 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     5101 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     5161 acgactcact atagggcgaa ttgggtacgt accgggcccc tagtatgtat gtaagttaat
     5221 aaaacccatt tttgcggaaa gtagataaaa aaaacatttt ttttttttac tgcactggat
     5281 atcattgaac ttatctgatc agttttaaat ttacttcgat ccaagggtat ttgatgtacc
     5341 aggttctttc gattacctct cactcaaaat gacattccac tcaaagtcag cgctgtttgc
     5401 ctccttctct gtccacagaa atatcgccgt ctctttcgcc gctgcgtccg ctatctcttt
     5461 cgccaccgtt tgtagcgtta cgtagcgtca atgtccgcct tcagttgcat tttgtcagcg
     5521 gtttcgtgac gaagctccaa gcggtttacg ccatcaatta aacacaaagt gctgtgccaa
     5581 aactcctctc gcttcttatt tttgtttgtt ttttgagtga ttggggtggt gattggtttt
     5641 gggtgggtaa gcaggggaaa gtgtgaaaaa tcccggcaat gggccaagag gatcaggagc
     5701 tattaattcg cggaggcagc aaacacccat ctgccgagca tctgaacaat gtgagtagta
     5761 catgtgcata catcttaagt tcacttgatc tataggaact gcgattgcaa catcaaattg
     5821 tctgcggcgt gagaactgcg acccacaaaa atcccaaacc gcaattgcac aaacaaatag
     5881 tgacacgaaa cagattattc tggtagctgt tctcgctata taagacaatt tttgagatca
     5941 tatcatgatc aagacatcta aaggcattca ttttcgacta tattcttttt tacaaaaaat
     6001 ataacaacca gatattttaa gctgatccta gatgcacaaa aaataaataa aagtataaac
     6061 ctacttcgta ggatacttcg gggtactttt tgttcggggt tagatgagca taacgcttgt
     6121 agttgatatt tgagatcccc tatcattgca gggtgacagc ggagcggctt cgcagagctg
     6181 cattaaccag ggcttcgggc aggccaaaaa ctacggcacg ctccggccac ccagtccgcc
     6241 ggaggactcc ggttcaggga gcggccaact agccgagaac ctcacctatg cctggcacaa
     6301 tatggacatc tttggggcgg tcaatcagcc gggctccgga tggcggcagc tggtcaaccg
     6361 gacacgcgga ctattctgca acgagcgaca cataccggcg cccaggaaac atttgctcaa
     6421 gaacggtgag tttctattcg cagtcggctg atctgtgtga aatcttaata aagggtccaa
     6481 ttaccaattt gaaactcagt ttgcggcgtg gcctatccgg gcgaactttt ggccgtgatg
     6541 ggcagttccg gtgccggaaa gacgaccctg ctgaatgccc ttgcctttcg atcgccgcag
     6601 ggcatccaag tatcgccatc cgggatgcga ctgctcaatg gccaacctgt ggacgccaag
     6661 gagatgcagg ccaggtgcgc ctatgtccag caggatgacc tctttatcgg ctccctaacg
     6721 gccagggaac acctgatttt ccaagccatg gtgcggatgc cacgacatct gacctatcgg
     6781 cagcgagtgg cccgcgtgga tcaggtgatc caggagcttt cgctcagcaa atgtcagcac
     6841 acgatcatcg gtgtgcccgg cagggtgaaa ggtctgtccg gcggagaaag gaagcgtctg
     6901 gcattcgcct ccgaggctct aaccgatccg ccgcttctga tctgcgatga gcccacctcc
     6961 ggactggact cctttaccgc ccacagcgtc gtccaggtgc tgaagaagct gtcgcagaag
     7021 ggcaagaccg tcatcctgac cattcatcag ccgtcatccg agctgtttga gctctttgac
     7081 aagatccttc tgatggccga gggcagggta gctttcttgg gcactcccag cgaagccgtc
     7141 gacttctttt cctagtgagt tcgatgtgtt tattaagggt atctagtatt acataacatc
     7201 tcaactccta tccagcgtgg gtgcccagtg tcctaccaac tacaatccgg cggactttta
     7261 cgtacaggtg ttggccgttg tgcccggacg ggagatcgag tcccgtgatc ggatcgccaa
     7321 gatatgcgac aattttgcca ttagcaaagt agcccgggat atggagcagt tgttggccac
     7381 caaaaatctg gagaagccac tggagcagcc ggagaatggg tacacctaca aggccacctg
     7441 gttcatgcag ttccgggcgg tcctgtggcg atcctggctg tcggtgctca aggaaccact
     7501 cctcgtaaaa gtgcgactta ttcagacaac ggtgagtggt tccagtggaa acaaatgata
     7561 taacgcttac aattcttgga aacaaattcg ctagatttta gatagaattg cctgattcca
     7621 cacccttctt agtttttttc aatgagatgt atagtttata gttttgcaga agataaataa
     7681 atttcattta actcgcgaat attaatgaga tgcgagtaac attttaattt gcagatggtt
     7741 gccatcttga ttggcctcat ctttttgggc caacaactca cgcaagtggg tgtgatgaat
     7801 atcaacggag ccatcttcct cttcctgacc aacatgacct ttcaaaacgt ctttgccacg
     7861 ataaatgtaa gtcatgttta gaatacattt gcatttcaat aatttactaa ctttctaatg
     7921 aatcgattcg atttaggtgt tcacctcaga gctgccagtt tttatgaggg aggcccgaag
     7981 tcgactttat cgctgtgaca catactttct gggcaaaacg attgccgaat tgccgctttt
     8041 tctcacagtg ccactggttt tcacggcgat tgcctatccg atgatcggac tgcgggccgg
     8101 agtgctgcac ttcttcaact gcctggcgct ggtcactctg gtggccaatg tgtcaacgtc
     8161 cttcggatat ctaatatcct gcgccagctc ctcgacctcg atggcgctgt ctgtgggtcc
     8221 gccggttatc ataccattcc tgctctttgg cggcttcttc ttgaactcgg gctcggtgcc
     8281 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct
     8341 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac
     8401 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc
     8461 gctggactac gtgggtctgg ccattctcat cgtgagcttc cgggtgctcg catatctggc
     8521 tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata actgcttgtt
     8581 ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa aaagctaatg
     8641 taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt atttcccctt
     8701 cgaacatccc cacaagtaga ctttggattt gtttctaacc aaaagactta cacacctgca
     8761 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     8821 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     8881 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     8941 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     9001 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     9061 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     9121 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     9181 ccagtgtttg ttccttgtgt agatgcatct caaaaaaatg gtgggcataa tagtgttgtt
     9241 tatatatatc aaaaataaca actataataa taagaataca tttaatttag aaaatgcttg
     9301 gatttcactg gaactaggct agcataactt cgtataatgt atgctatacg aagttatgct
     9361 agcggatcct ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt
     9421 ttcggcaaag taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta
     9481 gagaaaccaa ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc
     9541 ttttaataaa aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa
     9601 gattattata ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa
     9661 aatattgcat acgttgacga aacaaatttt cgttgcata
//
