LOCUS       sequence-24763-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Raasi S, Pickart CM
  TITLE     Ubiquitin chain synthesis.
  JOURNAL   Methods Mol Biol. 2005;301:47-55. doi: 10.1385/1-59259-895-1:047.
  PUBMED    15917625
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2005"; volume: "301:47-55. doi: 
            10.1385/1-59259-895-1"; pages: "047"
COMMENT     Sequence Label: pGEX-Ubc13
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(520..537)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(564..586)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgaacgcgcg aggcagatcg gtcagtcagt cacgatgcgg ccgctcgagt cgactcactc
       61 gggtttcttc tttgcataca atttcgtcca ttcgcgagcc ttagctttgg caccttgctc
      121 attctttatc caatcttcag cgacgtcatt agctagcgga tcattaggat tagggctagc
      181 caacagtgct tgaatggaca gcaggacggt tctgatctgt aaagcgggtg accaattagt
      241 tttaagcaca tcaagacata tacggcccaa cctatcaata ttgggatgat atatcttggt
      301 taaaaaacgt actttcggtg cttccattgg atagtcgtca ggcagatata attctaattc
      361 gaaaataccg tcttcatatg gtgactgttc tgggccttcg attgttactt ggaagtaacg
      421 taagttgtcg tcatgtggtt ctgctgtaat gccaggtact gggtcactta ctaatttctc
      481 agtttccttg attattctct tgggtaatga tgcggccggg gatccacgcg gaaccagatc
      541 cgattttgga ggatggtcgc caccaccaaa cgtggcttgc cagccctgca aaggccatgc
      601 tatatacttg ctggatttca agtacttatc aatttgtggg atagcttcaa tacgtttttt
      661 aaaacaaact antttgggaa cgcatccagg cacattgggt ccatgtataa acacatcaag
      721 agcgtctaca catgantccg nngggtcang ncccnnttna atnttttaag anaaannact
      781 ncaaantttc cnttnggggn gcngaaaaac actgaantca agctnntang catcca
//
