LOCUS       sequence-247640-        7387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Lin L, DeMartino GN, Greene WC
  TITLE     Cotranslational biogenesis of NF-kappaB p50 by the 26S proteasome.
  JOURNAL   Cell. 1998 Mar 20. 92(6):819-28.
  PUBMED    9529257
REFERENCE   2  (bases 1 to 7387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1998 
            Mar 20. 92(6):819-28."
COMMENT     Sequence Label: pEV-p105-WT
FEATURES             Location/Qualifiers
     source          1..7387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           294..333
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             369..401
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     intron          3348..3920
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(4052..4071)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4117..4172
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4171..4190)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4522..4657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4763..4780)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4934..5522)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5014..5033)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5693..6484,6485..6553))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6316..6335
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6554..6658)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6726..6744
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6782..6804)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6904..6923
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7042..7061
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7042..7060
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        7084..7387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg ggggatcttg
      301 gtggcgtgaa actcccgcac ctcttcggcc agcgccttgt agaagcgcgt atggcttcgt
      361 ggggatctat ggctagcatg actggtggac agcaaatggg tcgcggatcc atggcagacg
      421 atgatcccta cggaactggg caaatgtttc atttgaacac tgctttgact cactcaatat
      481 ttaatgcaga attatattca ccagaaatac cactgtcaac agatggccca taccttcaaa
      541 tattagagca accaaaacag aggggatttc gattccgcta tgtgtgtgaa ggcccatcac
      601 acggagggct tccgggagcc tctagtgaga agaacaagaa atcctaccca caggtcaaaa
      661 tttgcaacta tgtggggcct gcaaaggtta tcgttcagtt ggtcacaaat ggaaaaaaca
      721 tccacctgca cgcccacagc ctggtgggca agcactgtga ggacggggta tgcaccgtaa
      781 cagcaggacc caaggacatg gtggttggct ttgcaaacct gggaatactt catgtgacta
      841 agaaaaaggt atttgaaaca ctggaagcac ggatgacaga ggcgtgtatt aggggctata
      901 atcctggact tctggtgcat tctgaccttg cctatctaca agcagaaggc ggaggagacc
      961 ggcaactcac agacagagag aaggagatca tccgccaggc agccgtgcag cagaccaagg
     1021 agatggacct gagcgtggtg cgcctcatgt tcacagcctt cctccctgac agcactggca
     1081 gcttcactcg gagactggag cctgtggtgt cagacgccat ctatgatagc aaagccccga
     1141 atgcatccaa cctgaaaatc gtgagaatgg acagaacagc aggatgtgtg acgggagggg
     1201 aggagattta ccttctctgt gacaaggttc agaaagatga catccagatt cggttttatg
     1261 aagaggaaga aaatggcgga gtttgggaag gatttgggga cttttccccc acggatgttc
     1321 atagacagtt tgccattgtc ttcaaaacgc caaagtataa ggatgtcaac attacaaagc
     1381 cagcttccgt gtttgttcag cttcggagga aatcagacct ggaaactagt gaaccgaaac
     1441 cctttctcta ctaccctgaa atcaaagaca aagaggaagt gcaaaggaaa cgccagaagc
     1501 ttatgccgaa cttctcggac agcttcggcg gcggcagtgg agcgggagcc ggtggtggag
     1561 gcatgttcgg tagtggcggt ggcggaggga gtaccggaag ccctggccca gggtatggct
     1621 actcgaacta cggatttcct ccctacggtg ggattacatt ccatcccgga gtcacgaaat
     1681 ccaacgcagg ggtcacccat ggcaccataa acaccaaatt taaaaatggc cctaaagatt
     1741 gtgccaagag tgatgacgag gagagtctga ctctccctga gaaggaaact gaaggtgaag
     1801 ggcccagcct gcccatggcc tgcaccaaga cggaacccat cgccttggca tccaccatgg
     1861 aagacaagga gcaggacatg ggatttcagg ataacctctt tctcgagaag gctctgcagc
     1921 tcgccaggcg acacgccaac gcccttttcg actacgcagt gacgggggat gtgaagatgt
     1981 tgctggccgt gcaacgccat ctcaccgccg tgcaggatga gaatggggac agtgtcttac
     2041 acttagccat catccacctc cacgctcagc tcgtgaggga tctgctggaa gtcacatctg
     2101 gtttgatctc tgatgacatc atcaacatga gaaatgacct gtatcagaca cctctgcact
     2161 tggccgtgat caccaagcag gaagatgtag tagaggattt gctgagggtt ggggctgacc
     2221 tgagccttct ggaccgctgg ggcaactctg tcctgcacct agctgccaaa gaaggacacg
     2281 acagaatcct cagcatcctg ctcaagagca gaaaagcagc gccccttatc gaccacccca
     2341 atggggaagg tctaaatgcc atccacatag ctgtgatgag caatagcctg ccatgtctgc
     2401 tgctgctggt ggctgccggg gcagaagtca atgctcagga gcagaagtct gggcgcacag
     2461 cgctgcacct ggccgtggag tacgacaaca tctccttggc tggctgcctg cttctggagg
     2521 gtgatgccca cgtggacagt accacctatg atgggactac acctctgcat atagcggccg
     2581 gaagagggtc caccagactg gcagctcttc tcaaagcagc aggagcagac cccctggtgg
     2641 agaactttga gcctctctat gacctggacg actcttggga gaaggctgga gaagatgagg
     2701 gagtggtgcc aggtaccaca cccctggaca tggctgccaa ctggcaggta tttgacatac
     2761 taaatgggaa accgtatgag cctgtgttca catctgatga tatactacca caaggggaca
     2821 tgaagcagct gacagaagac acgaggctac aactctgcaa actgctggaa attcctgatc
     2881 cagacaaaaa ctgggccact ctggcacaga agttgggtct ggggatattg aacaatgcct
     2941 tccggctgag tcctgctcct tctaaaactc tcatggacaa ctatgaggtc tctgggggta
     3001 ccatcaaaga gctgatggag gccctgcaac agatgggcta cacagaggcc attgaagtga
     3061 tccaggcagc cttccgcacc ccggcaacca cagcctccag ccccgtgacc actgctcagg
     3121 tccactgtct gcctctctcg tcttcctcca cgaggcagca catagatgaa ctccgggata
     3181 gtgacagcgt ctgtgacagt ggtgtggaga catccttccg caaactcagc tttacagagt
     3241 ctcttactgg agacagccca ctgctatctc tgaacaaaat gccccacggt tatgggcagg
     3301 aaggacctat tgaaggcaaa atttagtcta gagctgagaa cttcagggtg agtttgggga
     3361 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
     3421 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     3481 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     3541 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     3601 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     3661 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     3721 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     3781 acaactacac cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     3841 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     3901 ccttcttctc tttcctacag ctcctgggca acgtgctggt tgttgtgctg tctcatcatt
     3961 ttggcaaaga attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     4021 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     4081 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     4141 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     4201 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     4261 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     4321 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     4381 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     4441 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     4501 ggagcttttt gcaaaagcct aggcctccaa aaaagcctct tcactacttc tggaatagct
     4561 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     4621 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     4681 tggttgctga ctaattgctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     4741 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4801 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4861 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4921 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4981 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5041 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5101 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5161 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5221 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5281 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5341 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5401 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5461 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5521 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5581 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5641 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     5701 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     5761 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     5821 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     5881 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     5941 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6001 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6061 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6121 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6181 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6241 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     6301 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     6361 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     6421 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     6481 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     6541 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     6601 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     6661 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     6721 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     6781 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6841 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6901 atgcggcatc agagcagatt gtactgagag tgcaccattc gacgctctcc cttatgcgac
     6961 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     7021 aatggtctgg cttatcgaaa ttaatacgac tcactatagg gagaccggaa ttcgagctcg
     7081 ccccgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     7141 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     7201 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     7261 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     7321 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     7381 taccatg
//
