LOCUS       sequence-247641-        7765 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human NEAT1_v1 short isoform 3.7kb insert in general cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Clemson CM, Hutchinson JN, Sara SA, Ensminger AW, Fox AH, Chess A, 
            Lawrence JB
  TITLE     An architectural role for a nuclear noncoding RNA: NEAT1 RNA is 
            essential for the structure of paraspeckles.
  JOURNAL   Mol Cell. 2009 Mar 27;33(6):717-26. doi: 
            10.1016/j.molcel.2009.01.026. Epub 2009 Feb 12.
  PUBMED    19217333
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2009.01.026"; journalName: "Mol Cell"; date: 
            "2009-03-27- 27"; volume: "33"; issue: "6"; pages: "717-26"
COMMENT     Sequence Label: pCRII_TOPO_hNEAT1
FEATURES             Location/Qualifiers
     source          1..7765
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     68..90
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     82..99
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     83..99
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     106..125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        106..124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(4066..4084)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4067..4084)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4102..4118)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4102..4118)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4115..4137)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4126..4142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4150..4156,4157..4174,4175..4180))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4195..4216
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4333..4350)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4504..5092)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4584..4603)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5263..6054,6055..6123))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5886..5905
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6141..6935)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6252..6271)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6862..6881
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     rep_origin      complement(7279..7707)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7389..7410)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7601..7620
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
       61 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta
      121 tagggcgaat tgggccctct agatgcatgc tcgagcggcc gccagtgtga tggatatctg
      181 cagaattcgc ccttagtcca gccggagtta gcgacaggga gggatgcgcg cctgggtgta
      241 gttgtggggg aggaagtggc tagctcaggg cttcagggga tagacaggga gaggtgactg
      301 agttagatga gacgaggggg cgggctgggg gtgcgagaag gaagcttggc aaggagacta
      361 ggtctagggg gaccacagtg gggcaggctg catggaaaat atccgcaggg tcccccaggc
      421 agaacagcca cgctccaggc caggctgtcc ctactgcctg gtggaggggg aacttgacct
      481 ctgggagggc gccgctcttg catagctgag cgagcccggg tgcgctggtc tgtgtggaag
      541 gaggaaggca gggagaggta gaaggggtgg aggagtcagg aggaataggc cgcagcagcc
      601 ctggaaatga tcaggaaggc aggcagtggg tgcagggctg caggagggcc gggagggcta
      661 atcttcaact tgtccatgcc agcagcccct ttttttccag accaagggct gtgaacccgc
      721 ctggggatga ggcctggtct tgtggaactg aacttagctc gacggggctg accgctctgg
      781 cccagggtgg tatgtaattt tcgctcggcc tgggacgggg cccaggccgg gcccagcctg
      841 gtggagcgtc caggtctggg tgcgaagcca ggcccctggg cggaggtgag gggtggtctg
      901 aggagtgatg tggagttaag gcgccatcct caccggtgac tggtgcggca cctagcatgt
      961 ttgacaggcg gggactgcga ggcacgctgc tcgggtgttg gggacaacat tgaccaacgc
     1021 tttattttcc aggtggcagt gctccttttg gacttttctc taggtttggc gctaaactct
     1081 tcttgtgagc tcactccacc ccttcttcct ccctttaact tatccattca cttaaaacat
     1141 tacctggtca tctggtaagc ccgggacagt aagccgagtg gctgttggag tcggtattgt
     1201 tggtaatggt ggaggaagag aggccttccc gctgaggctg gggtggggcg gatcggtgtt
     1261 gcttgcctgc agagagggtg gggagtgaat gtgcaccctt gggtgggcct gcagccatcc
     1321 agctgaaagt tacaaaaatg cttcatggac cgtggtttgt tactatagtg ttcctcatgg
     1381 cgagcagatg gaaccgggag acatggagtc cctggccagt gtgagtccta gcattgcagg
     1441 aggggagacc ctggaggaga gagcccgcct caattgatgc ctgcagattg aatttccaga
     1501 ggcttaggag gaggaagttc tccaatgttc tgtttccagg ccttgctcag gaagccctgt
     1561 attcaggagg ctaccattta aagtttgcag atgagcttat ggggggcaat cttaaaaagt
     1621 ccacagcaga tgcatccggc tcgaggggcc atcagctttg aataaatgct tgttccagag
     1681 cccatgaatg ccagcaggca cccctccttt cctggggtaa aggttttcag atgctgcatc
     1741 ttctaaattg agcctccggt catactagtt ttgtgcttgg aaccttgctt caagaagatc
     1801 cctaagctgt agaacatttt aacgttgatg ccacaacgca gattgatgcc ttgtagatgg
     1861 agcttgcaaa tggagccccg tgacctctca cctacccacc tgtttgcctg ccttcttgtg
     1921 cgtttctcgg agaagttctt agcctgatga aataacttgg ggcgttgaag agctgtttaa
     1981 ttttaaatgc cttagactgg ggatatatta gaggaagcag attgtcaaat taagggtgtc
     2041 attgtgttgt gctaaacgct gggagggtac aagttggtca ttcctaaatc tgtgtgtgag
     2101 aaatggcagg tctagtttgg gcattgtgat tgcattgcag attactagga gaagggaatg
     2161 gtgggtacac cggtagtgct cttttgttct tgcttcgttt ttttaaactt gaactttact
     2221 tcgttagatt tcaatacttt cttggcattc tagtaagagg accctgaggt gggagttgtg
     2281 ggggacgggg agaaggggac agcttggcac cggtcccgtg ggcgttgcag tgtgggggat
     2341 gggggtatgc agcttggcac tggtactggg agggatgagg gtgaagaagg ggagagggtt
     2401 ggttagagat acagtgtggg tggtgggggt ggtaggaaat gcaggttgaa gggaattctc
     2461 tggggctttg gggaatttag tgcgtgggtg agccaagaaa atactaatta ataatagtaa
     2521 gttgttagtg ttggttaagt tgttgcttgg aagtgagaag ttgcttagaa actttccaaa
     2581 gtgcttagaa ctttaagtgc aaacagacaa actaacaaac aaaaattgtt ttgctttgct
     2641 acaaggtggg gaagactgaa gaagtgttaa ctgaaaacag gtgacacaga gtcaccagtt
     2701 ttccgagaac caaagggagg ggtgtgtgat gccatctcac aggcagggga aatgtcttta
     2761 ccagcttcct cctggtggcc aagacagcct gtttcagagg gttgttttgt ttggggtgtg
     2821 ggtgttatca agtgaattag tcacttgaaa gatgggcgtc agacttgcat acgcagcaga
     2881 tcagcatcct tcgctgcccc ttagcaactt aggtggttga tttgaaactg tgaaggtgtg
     2941 attttttcag gagctggaag tcttagaaaa gccttgtaaa tgcctatatt gtgggctttt
     3001 aacgtattta agggaccact taagacgaga ttagatgggc tcttctggat ttgttcctca
     3061 tttgtcacag gtgtcttgtg attgaaaatc atgagcgaag tgaaattgca ttgaatttca
     3121 agggaattta gtatgtaaat cgtgccttag aaacacatct gttgtctttt ctgtgtttgg
     3181 tcgatattaa taatggcaaa atttttgcct atctagtatc ttcaaattgt agtctttgta
     3241 acaaccaaat aaccttttgt ggtcactgta aaattaatat ttggtagaca gaatccatgt
     3301 acctttgcta aggttagaat gaataattta ttgtattttt aatttgaatg tttgtgcttt
     3361 ttaaatgagc caagactaga ggggaaacta tcacctaaaa tcagtttgga aaacaagacc
     3421 taaaaaggga aggggatggg gattgtgggg agagagtggg cgaggtgcct ttactacatg
     3481 tgtgatctga aaaccctgct tggttctgag ctgcgtctat tgaattggta aagtaatacc
     3541 aatggctttt tatcatttcc ttcttccctt taagtttcac ttgaaatttt aaaaatcatg
     3601 gttattttta tcgttgggat ctttctgtct tctgggttcc attttttaaa tgtttaaaaa
     3661 tatgttgaca tggtagttca gttcttaacc aatgacttgg ggatgatgca aacaattact
     3721 gtcgttggga tttagagtgt attagtcacg catgtatggg gaagtagtct cgggtatgct
     3781 gttgtgaaat tgaaactgta aaagtagatg gttgaaagta ctggtatgtt gctctgtatg
     3841 gtaagaacta attctgttac gtcatgtaca taattactaa tcacttttct tcccctttac
     3901 agcacaaata aagtttgagt tctaaactca ttagaattgt tgtattgcta tgttacattt
     3961 ctcgacccct atcacattgc cttcataagg gcgaattcca gcacactggc ggccgttact
     4021 agtggatccg agctcggtac caagcttgat gcatagcttg agtattctat agtgtcacct
     4081 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     5101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     5161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     5221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     5281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     5341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     5401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     5461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     5521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     5581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     5641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     5701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     5761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     5821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     5881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     5941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     6001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     6061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     6121 catactcttc ctttttcaat tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     6181 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     6241 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     6301 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     6361 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgcgcgc cttgagcctg
     6421 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     6481 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     6541 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     6601 ttctcggcag gagcaaggtg ggatgacagg agatcctgcc ccggcacttc gcccaatagc
     6661 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     6721 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     6781 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6841 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6901 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6961 tgatcagatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc catccagttt
     7021 actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc cggttcgctt
     7081 gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca agctacctgc
     7141 tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac attcatccgg
     7201 ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta gcagcccttg
     7261 cgccctgaat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     7321 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     7381 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     7441 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     7501 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     7561 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     7621 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     7681 cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc aggctgcgca actgttggga
     7741 agggcgatcg gtgcgggcct cttcg
//
